Whole-genome amplification by improved primer extension preamplification PCR (I-PEP-PCR)

Cold Spring Harbor Protocols

Volumn 3, Issue 1, 2008, Pages

  Arneson, Nona ^a   Hughes, Simon ^a   Houlston, Richard ^a   Done, Susan ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; DNA POLYMERASE; PHOSPHODIESTERASE I; PRIMER DNA; SPLEEN EXONUCLEASE; TAQ POLYMERASE;

AGAR GEL ELECTROPHORESIS; ARTICLE; DEGENERATE OLIGONUCLEOTIDE PRIMED POLYMERASE CHAIN REACTION; DNA DETERMINATION; ENZYME ACTIVITY; GENE AMPLIFICATION; IMPROVED PRIMER EXTENSION PREAMPLIFICATION POLYMERASE CHAIN REACTION; METHODOLOGY; POLYMERASE CHAIN REACTION; TISSUE FIXATION; WHOLE GENOME AMPLIFICATION;

EID: 44949149461     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot4921     Document Type: Article

References (5)

1. Dietmaier, W., Hartmann, A., Wallinger, S., Heinmoller, E., Kerner, T., Endl, E., Jauch, K.W., Hofstadter, F., and Ruschoff, J. 1999. Multiple mutation analyses in single tumor cells with improved whole genome amplification. Am. J. Pathol. 154: 83-95.
2. Hughes, S., Arneson, N., Done, S., and Squire, J. 2005. The use of whole genome amplification in the study of human disease. Prog. Biophys. Mol. Biol. 88: 173-189.
3. Obermann, E.C., Junker, K., Stoehr, R., Dietmaier, W., Zaak, D., Schubert, J., Hofstaedter, F., Knuechel, R., and Hartmann, A. 2003. Frequent genetic alterations in flat urothelial hyperplasias and concomitant papillary bladder cancer as detected by CGH, LOH and FISH analyses. J. Pathol. 199: 50-57.
4. Sun, F., Arnheim, N., and Waterman, M.S. 1995. Whole genome amplification of single cells: Mathematical analysis of PEP and tagged PCR. Nucleic Acids Res. 23: 3034-3040.
5. Zhang, L., Cui, X., Schmitt, K., Hubert, R., Navidi, W., and Arnheim, N. 1992. Whole genome amplification from a single cell: Implications for genetic analysis. Proc. Natl. Acad. Sci. 89: 5847-5851.