Whole-genome amplification by degenerate oligonucleotide primed PCR (DOP-PCR)

Cold Spring Harbor Protocols

Volumn 3, Issue 1, 2008, Pages

  Arneson, Nona ^a   Hughes, Simon ^a   Houlston, Richard ^a   Done, Susan ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ETHIDIUM BROMIDE; GENOMIC DNA;

ARTICLE; CLINICAL PROTOCOL; DEGENERATE OLIGONUCLEOTIDE PRIMED POLYMERASE CHAIN REACTION; DNA DEGRADATION; DNA EXTRACTION; DNA HYBRIDIZATION; DNA PURIFICATION; FLUORESCENCE; GEL ELECTROPHORESIS; GENE AMPLIFICATION; HUMAN; MOLECULAR WEIGHT; POLYMERASE CHAIN REACTION; PROTECTIVE CLOTHING; SAFETY;

EID: 44949140878     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot4919     Document Type: Article

References (6)

1. Bannai, M., Higuchi, K, Akesaka, T., Furukawa, M., Yamaoka, M., Sato, K., and Tokunaga, K. 2004. Single-nucleotide-polymorphism genotyping for whole-genome-amplified samples using automated fluorescence correlation spectroscopy. Anal. Biochem. 327: 215-221.
2. Cheung, V.G. and Nelson, S.F. 1996. Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performedon less than one nanogram of genomic DNA. Proc. Natl. Acad Sci. 93: 14676-14679.
3. Grant, S.F., Steinlicht, S., Nentwich, U., Kern, R., Burwinkel, B., and Tolle, R. 2002. SNP genotyping on a genome-wide amplified DOP-PCR template. Nucleic Acids Res. 30: e125. doi: 10.10934nar/ gnf125.
4. Ng, G., Roberts, I., and Coleman, N. 2005. Evaluation of 3 methods of whole-genome amplification for subsequent metaphase comparative genomic hybridization. Diagn. Mol. Pathol. 14: 203-212.
5. Peng, D.F., Sugihara, H., Mukaisho, K., Tsubosa, Y., and Hattori, T. 2003. Alterations of chromosomal copy number during progression of diffusetype gastric carcinomas: Metaphase- and array-based comparative genomic hybridization analyses of multiple samples from individual tumours. J. Pathol. 201: 439450.
6. Telenius, H., Carter, N.P., Bebb, C.E., Nordenskjold, M., Ponder, BA., and Tunnacliffe, A. 1992. Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer. Genomics 13: 718-725.