In vitro histone methyltransferase assay

Cold Spring Harbor Protocols

Volumn 3, Issue 2, 2008, Pages

  Fingerman, Ian M ^a   Du, Hai Ning ^a   Briggs, Scott D ^a  

^a Purdue University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CELL EXTRACT; HISTONE METHYLTRANSFERASE; S ADENOSYLMETHIONINE;

ARTICLE; BACTERIAL CELL; CELL COUNT; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FLUOROGRAPHY; IN VITRO STUDY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; SCINTILLATION COUNTING;

EID: 44949139930     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot4939     Document Type: Article

References (10)

1. Briggs, S.D., Bryk, M., Strahl, B.D., Cheung, W.L., Davie, J.K., Dent, S.Y.R., Winston, F., and Allis, C.D. 2001. Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae. Genes & Dev. 15: 3286-3295.
2. Briggs, S.D., Xiao, T.J., Sun, Z.W., Caldwell, J.A., Shabanowitz, J., Hunt, D.F., Allis, C.D., and Strahl, B.D. 2002. Gene silencing: Trans-histone regulatory pathway in chromatin. Nature 418: 498.
3. Fingerman, I.M., Li, H.C., and Briggs, S.D. 2007. A charge-based interaction between histone H4 and Dot1 is required for H3K79 methylation and telomere silencing: Identification of a new trans-histone pathway. Genes & Dev. 21: 2018-2029.
4. Krogan, N.J., Dover, J., Khorrami, S., Greenblatt, J.F., Schneider, J., Johnston, M., and Shilatifard, A. 2002. COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression. J. Biol. Chem 277: 10753-10755.
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6. Mizzen, C.A., Brownell, J.E., Cook, R.G., and Allis, C.D. 1999. Histone acetyltransferases: Preparation of substrates and assay procedures. Methods Enzymol. 304: 675-696.
7. Nagy, P.L., Griesenbeck, J., Kornberg, R.D., and Cleary, M.L. 2002. A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc. Natl. Acad. Sci. 99: 90-94.
8. Roguev, A., Schaft, D., Shevchenko, A., Pijnappel, W.W., Wilm, M., Aasland, R., and Stewart, A.F. 2001. The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J. 20: 7137-7148.
9. Schnitzler, G.R. 2000. Isolation of histones and nucleosome cores from mammalian cells.. In Current Protocols in Molecular Biology (eds. F.M. Ausubel et al.), pp. 21.5.1-21.5.12. John Wiley and Sons, New York.
10. Strahl, B.D., Briggs, S.D., Brame, C.J., Caldwell, J.A., Koh, S.S., Ma, H., Cook, R.G., Shabanowitz, J., Hunt, D.F., Stallcup, M.R., et al. 2001. Methylation of histone H4 at arginine 3 occurs in vivo and is mediated by the nuclear receptor coactivator PRMT1. Curr.Biol. 11: 996-1000.