Overexpression of a delayed early gene hlg1 of temperate mycobacteriophage L1 is lethal to both M. smegmatis and E. coli

Journal of Biochemistry and Molecular Biology

Volumn 41, Issue 5, 2008, Pages 363-368

  Chattoraj, Partho ^a   Ganguly, Tridib ^a   Nandy, Ranjan Kumar ^b   Sau, Subrata ^a  

^a BOSE INSTITUTE   (India)

^b NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES   (India)

Author keywords

Delayed early gene; Growth retardation; Host lethal gene; Mycobacteriophage L1

Indexed keywords

ESCHERICHIA COLI; MYCOBACTERIUM PHAGE L5;

EID: 44849137752     PISSN: 12258687     EISSN: 02191024     Source Type: Journal    
DOI: 10.5483/bmbrep.2008.41.5.363     Document Type: Article

References (15)

1. Sau, S., Chattoraj, P., Ganguly, T., Chanda, P.K. and Mandal, N.C. (2008) Inactivation of indispensable bacterial proteins by early proteins of bacteriophages: implication in antibacterial drug discovery. Curr. Protein Pept. Sci. (in press).
2. Liu, J., Dehbi, M., Moeck, G., Arhin, F., Bauda, P., Bergeron, D., Callejo, M., Ferretti, V., Ha, N., Kwan, T., McCarty, J., Srikumar, R., Williams, D., Wu, J. J., Gros, P., Pelletier, J. and DuBow, M. (2004) Antimicrobial drug discovery through bacteriophage genomics. Nat. Biotechnol. 22, 185-191.
3. Hatfull, G. F. (2000) Molecular genetics of mycobacteriophages; in Molecular Genetics of Mycobacteria, Hatfull, G. F. and Jacobs, W. R., Jr. (eds.), ASM Press, Washington D.C., pp. 37-54.
4. Ganguly, T., Bandhu, A., Chattoraj, P., Chanda. P. K., Das, M., Mandal, N. C. and Sau, S. (2007) Repressor of temperate mycobacteriophage L1 harbors a stable C-terminal domain and binds to different asymmetric operator DNAs with variable affinity. Virol. J. 4, 64.
5. Chaudhuri, B., Sau, S., Datta, H. J. and Mandal, N. C. (1993) Isolation, characterization, and mapping of temperature-sensitive mutations in the genes essential for lysogenic and lytic growth of the mycobacteriophage L1. Virology 194, 166-172.
6. Sun, X., Gohler, A., Heller, K. J. and Neve, H. (2006) The ltp gene of temperate Streptococcus thermophilus phage TP-J34 confers superinfection exclusion to Streptococcus thermophilus and Lactococcus lactis. Virology. 350, 146-157.
7. Clapper, B., Tu, A. H., Elgavish, A. and Dybvig, K. (2004) The vir gene of bacteriophage MAV1 confers resistance to phage infection on Mycoplasma arthritidis. J. Bacteriol. 186, 5715-5720.
8. Pedruzzi, I., Rosenbusch, J. P. and Locher, K. P. (1998) Inactivation in vitro of the Escherichia coli outer membrane protein FhuA by a phage T5-encoded lipoprotein. FEMS Microbiol. Lett. 168, 119-125.
9. Sambrook, J., and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, CSH, New York, USA.
10. Triccas, J. A., Parish, T., Britton, W. J. and Gicquel, B. (1998) An inducible expression system permitting the efficient purification of a recombinant antigen from Mycobacterium smegmatis. FEMS Microbiol. Lett. 167, 151-156.
11. Bachrach, G., Colston, M. J., Bercovier, H., Bar-Nir, D., Anderson, C. and Papavinasasundaram, K. G. (2000) A new single-copy mycobacterial plasmid, pMF1, from Mycobacterium fortuitum which is compatible with the pAL5000 replicon. Microbiology. 146, 297-303.
12. Blokpoel, M. C., Murphy, H. N., O'Toole, R., Wiles, S., Runn, E. S., Stewart, G. R., Young, D.B. and Robertson, B. D. (2005) Tetracycline-inducible gene regulation in mycobacteria. Nucleic Acids Res. 33, e22.
13. Ganguly, T., Chattoraj, P., Das, M., Chanda, P. K., Mandal, N. C., Lee, C. Y. and Sau, S. (2004) A point mutation at the C-terminal half of the repressor of temperate mycobacteriophage L1 affects its binding to the operator DNA. J. Biochem. Mol. Biol. 37, 709-714.
14. Chanda, P. K., Ganguly, T., Das, M., Lee, C. Y., Luong, T. T. and Sau, S. (2007) Detection of antistaphylococcal and toxic compounds by biological assay systems developed with a reporter Staphylococcus aureus strain harboring a heat inducible promoter-lacZ transcriptional fusion. J. Biochem. Mol. Biol. 40, 936-943.
15. Wayne, L. G. (1977) Synchronized replication of Mycobacterium tuberculosis. Infect Immun. 17, 528-530.