Unique pattern of nuclear TdT immunofluorescence distinguishes normal precursor B cells (hematogones) from lymphoblasts of precursor B-lymphoblastic leukemia

American Journal of Clinical Pathology

Volumn 129, Issue 5, 2008, Pages 700-705

  Hurford, Matthew T ^a,e   Altman, Arnold J ^b   DiGiuseppe, Joseph A ^c,d   Sherburne, Bradford J ^c   Rezuke, William N ^c  

^a TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Division of Pediatric Infectious Diseases   (United States)

^c HARTFORD HOSPITAL   (United States)

^d UNIVERSITY OF CONNECTICUT SCHOOL OF MEDICINE   (United States)

^e TEMPLE UNIVERSITY HOSPITAL   (United States)

Author keywords

Hematogones; Immunofluorescence; Normal precursor B cells; Precursor B lymphoblastic leukemia; TdT; Terminal deoxynucleotidyl transferase

Indexed keywords

DNA NUCLEOTIDYLEXOTRANSFERASE;

ACUTE LYMPHOCYTIC LEUKEMIA; ADOLESCENT; ADULT; AGED; ARTICLE; BLOOD SAMPLING; BONE MARROW BIOPSY; CELL NUCLEUS; CHILD; CLINICAL ARTICLE; DIAGNOSTIC PROCEDURE; FEMALE; FLOW CYTOMETRY; HEMATOGONE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE; IMMUNOPHENOTYPING; LYMPHOBLAST; MALE; MORPHOLOGY; PRIORITY JOURNAL; STEM CELL; B CELL LEUKEMIA; B LYMPHOCYTE; CANCER STEM CELL; FLUORESCENT ANTIBODY TECHNIQUE; INFANT; METABOLISM; MIDDLE AGED; PATHOLOGY; PRESCHOOL CHILD;

ADOLESCENT; ADULT; AGED; B-LYMPHOCYTES; CELL NUCLEUS; CHILD; CHILD, PRESCHOOL; DNA NUCLEOTIDYLEXOTRANSFERASE; FEMALE; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; INFANT; MALE; MIDDLE AGED; NEOPLASTIC STEM CELLS; PRECURSOR B-CELL LYMPHOBLASTIC LEUKEMIA-LYMPHOMA; STEM CELLS;

EID: 44849099983     PISSN: 00029173     EISSN: None     Source Type: Journal    
DOI: 10.1309/ANERT51H38TUEC45     Document Type: Article

References (19)

1. Longacre TA, Foucar K, Crago S, et al. Hematogones: a multiparameter analysis of bone marrow precursor cells. Blood. 1989;73:543-552.
2. Caldwell CW, Poje E, Helikson MA. B-cell precursors in normal pediatric bone marrows. Am J Clin Pathol. 1991;95:816-823.
3. Leitenberg D, Rappeport JM, Smith BR. B-cell precursor bone marrow reconstitution after bone marrow transplantation. Am J Clin Pathol. 1994;102:231-236.
4. van den Doel L, Pieters R, Huismans D, et al. Immunological phenotype of lymphoid cells in regenerating bone marrow of children after treatment of acute lymphoblastic leukemia. Eur J Haematol. 1988;41:170-175.
5. Sandhaus LM, Chen TL, Ettinger LJ, et al. Significance of increased proportion of CD10-positive cells in nonmalignant bone marrows of children. Am J Pediatr Hematol Oncol. 1993;15:65-70.
6. McKenna RW, Washington LT, Aquino DB, et al. Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry. Blood. 2001;98:2498-2507.
7. Van Lochem EG, Wiegers YM, van den Beemd R, et al. Regeneration pattern of precursor B cells in bone marrow of acute lymphoblastic leukemia patients depends on the type of preceding chemotherapy. Leukemia. 2000;14:688-695.
8. Styass SA, Schumacher HR, Kenklis TP, et al. Terminal deoxynucleotidyl transferase immunofluorescence of bone marrow smears. Am J Clin Pathol. 1979;72:898-903.
9. Kurec AS, Davey FR. Terminal deoxynucleotidyl transferase. Hum Pathol. 1981;12:867-869.
10. Bollum FJ. Terminal deoxynucleotidyl transferase as a hematopoietic cell marker. Blood. 1979;54:1203-1215.
11. Greenwood MF, Coleman MS, Hutton JJ, et al. Terminal deoxynucleotidyl transferase distribution in neoplastic and hematopoietic cells. J Clin Invest. 1977;91:640-646.
12. Rimsza LM, Larson RS, Winter SS, et al. Benign hematogone-rich lymphoid proliferations can be distinguished from B-lineage acute lymphoblastic leukemia by integration of morphology, immunophenotype, adhesion molecule expression, and architectural features. Am J Clin Pathol. 2000;114:66-75.
13. Dworzak MN, Fritsch G, Fleischer C, et al. Multiparameter phenotype mapping of normal and post-chemotherapy B lymphopoiesis in pediatric bone marrow. Leukemia. 1997;11:1266-1273.
14. Wells DA, Sale GE, Shulman HM, et al. Multidimensional flow cytometry of marrow can differentiate leukemic from normal lymphoblasts and myeloblasts after chemotherapy and bone marrow transplantation. Am J Clin Pathol. 1998;110:84-94.
15. Weir EG, Cowan K, LeBeau P, et al. A limited antibody panel can distinguish B-precursor acute lymphoblasts leukemia from normal B precursors with four color flow cytometry: implications for residual disease detection. Leukemia. 1999;13:558-567.
16. Campana D, Coustan-Smith E. Detection of minimal residual disease in acute leukemia by flow cytometry. Cytometry. 1999;38:139-152.
17. Washington L, Ansari M, Picker L, et al. Immunophenotypic analysis of B-cell precursors (BCP) in 661 bone marrow specimens by 4 color flow cytometry (FC) [abstract]. Mod Pathol. 1999;12:148A.
18. Farahat N, Lens D, Zomas A, et al. Quantitative flow cytometry can distinguish between normal and leukemic B-cell precursors. Br J Haematol. 1995;91:640-646.
19. Rimsza LM, Viswanatha DS, Winter SS, et al. The presence of CD34+ cell clusters predicts impending relapse in children with acute lymphoblastic leukemia receiving maintenance chemotherapy. Am J Clin Pathol. 1998;110:313-320.