Simultaneous detection of one RNA and one DNA virus from naturally infected citrus plants using duplex PCR technique

Current Science

Volumn 94, Issue 10, 2008, Pages 1314-1318

  Ghosh, D K ^a   Aglave, Balaji ^a   Baranwal, V K ^b  

^a NATIONAL RESEARCH CENTRE FOR CITRUS   (India)

^b INDIAN AGRICULTURAL RESEARCH INSTITUTE   (India)

Author keywords

Citrus plants; Detection protocol; Duplex PCR technique; RNA and DNA virus

Indexed keywords

APHIDIDAE; CITRUS; CITRUS MOSAIC VIRUS (BADNAVIRUS); CITRUS SINENSIS; CITRUS TRISTEZA; DNA VIRUSES; RNA VIRUSES;

EID: 44849097541     PISSN: 00113891     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Garnsey, S. M., Gumpf, D. J., Roistacher, C. N., Civerolo, E. L., Lee, R. F., Yokomi, R. K. and Bar-Joseph, M., Towards a standardized evaluation of the biological properties of citrus tristeza virus. Phytophylactica, 1987, 19, 151-157.
2. Dakshinamurti, V. and Reddy, G. S., Moasic - A transmissible disorder of sweet oranges. Indian Phytopathol., 1975, 28, 398-399.
3. Ahlawat, Y. S., Chenulu, V. V., Vishwanath, S. M. and Pandey, P. K., Studies on a mosaic disease of citrus. Curr. Sci., 1985, 54, 873-874.
4. Ghosh, D. K., Aglave, B., Bhanare, K. and Baranwal, V. K., PCR based detection of Citrus yellow mosaic disease from Vidarbha region of Maharashtra. Indian Phytopathol., 2007, 60, 520-526.
5. Ghosh, D. K., Das, A. K. and Singh, S., Individual and mixed infection of CTV and greening Liberobacter and their association with sweet orange decline in the state of Maharashtra. J. Mycol. Plant Pathol., 2003, 33, 69-72.
6. Ahlawat, Y. S., Pant, R. P., Lockhart, B. E. L., Srivastava, M., Chakraborty, N. K. and Varma, A., Association of badnavirus with citrus mosaic disease in India. Plant Dis., 1996, 80, 590-592.
7. Huang, Q. and Hartung, J. S., Cloning and sequence analysis of an infectious clone of citrus yellow mosaic virus that can infect sweet orange via Agrobacterium mediated inoculation. J. Gen. Virol., 2001, 82, 2549-2558.
8. Mathews, D. M., Riley, K. and Dodds, J. A., Comparison of detection of CTV in field trees during months of non-optimal titer. Plant Dis., 1997, 81, 525-529.
9. Nemichov, L., Hadidi, A., Candresse, T., Foster, J. A. and Verdervskaya, T., Sensitive detection of apple chlorotic leaf spot virus from infected apple or peach tissue using RT-PCR, IC-RT-PCR or mutiplex IC-RT-PCR. Acta Hortic., 1995, 386, 51-62.
10. Nie, X. and Singh, R. P., A novel usage of random primers for mutiplex RT-PCR detection of virus and viroids in aphids, leaves and tubers. J. Virol. Methods, 2001, 91, 37-49.
11. Singh, R. P., Kurz, J. and Boiteau, G., Detection of stylet borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction. J. Virol. Methods, 1996, 59, 189-196.
12. Grieco, F. and Gallitelli, D., Multiplex reverse transcriptase chain reaction applied to virus detection in globe artichoke. J. Phytopathol., 1999, 147, 183-185.
13. Bertolini, E., Olmos, A., Carmen Martiinez, M., Mariia Teresa, G. and Cambra, M., Single-step multiplex RT-PCR for simultaneous and colorimetric detection of six RNA viruses in olive trees. J. Virol. Methods, 2001, 96, 33-41.
14. Shraman, M., Thomas, J. and Dietzgen, R. G., Development of a multiplex immunocapture PCR with colorimetric detection for viruses of banana. J. Virol. Methods, 2000, 89, 75-88.
15. James, D., A simple and reliable protocol for the detection of apple stem grooving virus by RT-PCR and in a multiplex PCR assay. J. Virol. Methods, 1999, 83, 1-9.
16. Hung, T. H., Wu, M. L. and Su, H. J., A rapid method based on the one-step reverse transcriptase polymerase chain reaction (RT-PCR) technique for detection of different strains of CTV. J. Phytopathol., 2000, 148, 469-475.
17. Baranwal, V. K., Majumder, S., Ahlawat, Y. S. and Singh, R. P., Sodium sulphite yields improved DNA of higher stability for PCR detection of citrus yellow mosaic virus from citrus leaves. J. Virol. Methods, 2003, 112, 153-156.