Refinement of a commercial bench-top relaxin assay for pregnancy diagnosis using urine from domestic and nondomestic felids

Journal of Zoo and Wildlife Medicine

Volumn 39, Issue 2, 2008, Pages 170-179

  Harris, Laurie A ^a,c   Steinetz, Bernard G ^b   Bond, Jennifer B ^a   Lasano, Sally ^b   Swanson, William F ^a  

^a Cincinnati Zoo and Botanical Garden   (United States)

^b NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c NONE   (United States)

Author keywords

Cats; Conservation; Pregnancy; Relaxin; Urine

Indexed keywords

BIOLOGICAL MARKER; RELAXIN;

ANALYTICAL EQUIPMENT; ANIMAL; ANIMAL DISEASE; ARTICLE; BREEDING; CAT; FELIDAE; FEMALE; METHODOLOGY; PHYSIOLOGY; PREGNANCY; PREGNANCY TEST; RADIOIMMUNOASSAY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; STANDARD; URINE;

ANIMALS; BIOLOGICAL MARKERS; BREEDING; CATS; FELIDAE; FEMALE; PREGNANCY; PREGNANCY TESTS; PREGNANCY, ANIMAL; RADIOIMMUNOASSAY; REAGENT KITS, DIAGNOSTIC; RELAXIN; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY;

ACINONYX JUBATUS; FELIDAE; FELIS CATUS; FELIS MARGARITA; MAMMALIA; OTOCOLOBUS MANUL; PANTHERA; PANTHERA LEO;

EID: 44649194981     PISSN: 10427260     EISSN: None     Source Type: Journal    
DOI: 10.1638/2007-0093R.1     Document Type: Article

References (16)

1. Addiego, L. A., T. Tsutsui, D. R. Stewart, and G. H. Stabenfeldt. 1987. Determination of the source of immunoreactive relaxin in the cat. Biol. Reprod. 37: 1165-1169.
2. Bani, D. 1997. Relaxin: a pleiotropic hormone. Gen. Pharmacol. 28: 13-22.
3. Berzofsky, J. A., S. L. Epstein, and I. J. Berkower. 1989. Antigen-antibody interactions and monoclonal antibodies. In: Paul, W. E. (ed.). Fundamental Immunology, 2nd ed. Raven Press, New York, New York. Pp. 315-356.
4. Brown, J. L. 2006. Comparative endocrinology of domestic and nondomestic felids. Theriogenology 66: 25-36.
5. de Haas van Dorsser, F. J., S. Lasano, and B. G. Steinetz. 2007. Pregnancy diagnosis in cats using a rapid, bench-top kit to detect relaxin in urine. Reprod. Domest. Anim. 42: 111-112.
6. de Haas van Dorsser, E J., W. F. Swanson, S. Lasano, and B. G. Steinetz. 2006. Development, validation, and application of a urinary relaxin radioimmunoassay for the diagnosis and monitoring of pregnancy in felids. Biol. Reprod. 74: 1090-1095.
7. Klonisch, T., S. Hombach-Klonisch, C. Froehlich, J. Kauffold, K. Steger, B. Huppertz, and B. Fischer. 1999. Nucleic acid sequence of feline preprorelaxin and its localization within the feline placenta. Biol. Reprod. 60: 305-311.
8. Sherwood, O. D. 2004. Relaxin's physiological roles and other diverse actions. Endocr. Rev. 25: 205-234.
9. Steinetz, B. G., J. L. Brown, T. L. Roth, and N. Czekala. 2005. Relaxin concentrations in serum and urine of endangered species: correlation with physiologic events and use as a marker of pregnancy. Ann. N Y Acad. Sci. 1041: 367-378.
10. Steinetz, B. G., E. E. Büllesbach, L. T. Goldsmith, C. Schwabe, and G. Lust. 1996. Use of synthetic canine relaxin to develop a rapid homologous radioimmunoassay. Biol. Reprod. 54: 1252-1260.
11. Stewart, D. R., and G. H. Stabenfeldt. 1985. Relaxin activity in the pregnant cat. Biol. Reprod. 32: 848-854.
12. Sunquist, M., and F. Sunquist. 2002. Wild Cats of the World. Univ. Chicago Press, Chicago, Illinois. P. 452.
13. Swanson, W. F. 2003. Research in nondomestic species: experiences in reproductive physiology research for conservation of endangered felids. ILAR J. 44: 307-316.
14. Swanson, W. F., T. L. Roth, and D. E. Wildt. 1994. In vivo embryogenesis, embryo migration and embryonic mortality in the domestic cat. Biol. Reprod. 51: 452-464.
15. Wood, W. G. 1993. Limitations and drawbacks: matrix effects. In: Masseyeff, R. F., W. H. Albert, and N. A. Staines (eds.). Methods of Immunological Analysis. VCH Publishers, New York, New York. Pp. 604-617.
16. Zambelli, D., and F. Prati. 2006. Ultrasonography for pregnancy diagnosis and evaluation in queens. Theriogenology 66: 135-144.