Purification and characterisation of a fructosyltransferase from Rhodotorula sp.

Applied Microbiology and Biotechnology

Volumn 79, Issue 4, 2008, Pages 589-596

  Hernalsteens, Saartje ^a   Maugeri, Francisco ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Enzyme characterisation; Enzyme kinetics; Fructooligosaccharides; Rhodotorula sp.

Indexed keywords

ENZYME KINETICS; ETHANOL; PH EFFECTS; PRECIPITATION (CHEMICAL);

ENZYME CHARACTERIZATION; FRUCTOOLIGOSACCHARIDES; RHODOTORULA SP.;

BACTERIA;

BACTERIAL ENZYME; DIMER; FRUCTOFURANOSIDASE; FRUCTOSE OLIGOSACCHARIDE; FRUCTOSYLTRANSFERASE; PREBIOTIC AGENT; PROBIOTIC AGENT; SUCROSE; TRANSFERASE;

COOPERATIVE BEHAVIOR; ELECTROKINESIS; ENZYME; ENZYME ACTIVITY; GROWTH; SULFATE; TEMPERATURE; YEAST;

ACIDITY; ALLOSTERISM; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOTECHNOLOGY; CELL SEPARATION; CENTRIFUGATION; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SUBSTRATE; GEL FILTRATION; HIGH TEMPERATURE; MICHAELIS MENTEN KINETICS; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; RHODOTORULA; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

BETA-FRUCTOFURANOSIDASE; CHROMATOGRAPHY, ION EXCHANGE; ENZYME STABILITY; FUNGAL PROTEINS; HEXOSYLTRANSFERASES; KINETICS; MOLECULAR WEIGHT; OLIGOSACCHARIDES; RHODOTORULA; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 44649153729     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1470-x     Document Type: Article

References (27)

1. Belcarz A, Ginalska G, Lobarzewski J, Penel C (2002) The novel non-glycosylated invertase from Candida utilis (the properties and the conditions of production and purification). Biochimica et Biophysica Acta 1594:40-53
2. Chen W, Liu C (1996) Production of b-fructofuranosidase by Aspergillus japonicus. Enzyme Microb Technol 18:153-160
3. Ghazi I, Fernandez-Arrojo L, Garcia-Arellano H, Ferrer M, Ballesteros A, Plou FJ (2007) Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus. J Biotechnol 128:204-211
4. Gibson GR, Roberfroid MD (1995) Dietary modulation of the colonic microbiota: introducing the concept of prebiotics. J Nutr 125:1401-1412
5. Hames BD, Rickwood D (1990) Gel electrophoresis of proteins-a practical approach. IRL Press, Oxford, p 383
6. Harris ELV, Angal S (1995) Protein purification methods: a practical approach. IRL Press, New York, p 317
7. Hayashi S, Nonoguchi M, Takasaki Y, Ueno H, Imada K (1991) Purification and properties of b-fructofuranosidase from Aurebasidium sp. ATCC 20524. J Ind Microbiol 7:251-256
8. Hidaka H, Hirayama M, Sumi N (1988) A fructooligosaccharide-producing enzyme from Aspergillus niger ATCC 20611. Agric Biol Chem 52(05):1181-1187
9. L'Hocine L, Wang Z, Jiang B, Xu S (2000) Purification and partial characterization of fructosyltransferase and invertase from Aspergillus niger AS0023. J Biotechnol 81:73-84
10. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature 277:680-685
11. Lowry OH, Rosebroght NJ, Farr AI, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265-275
12. Maugeri F, Hernalsteens S (2007) Screening of yeast strains for transfructosylating activity. J Mol Catal B Enzym 49:43-49
13. Monsan P, Paul F (1995) Enzymatic synthesis of oligosaccharides. FEMS Microbiol Rev 16(2-3):187-190
14. Nelson N (1944) A fotometric adaptation of Somogyi method for the determination of glucose. J Biol Chen 153:375-380
15. Onishi N, Tanaka T (1996) Purification and properties of galacto- and gluco-oligosaccharide-producing b-glycosidase from Rhodotorula minuta IFO879. J Ferment Bioeng 82:439-443
16. Ozimek LK, Euverink GJW, van der Maarel MJEC, Dijkhuizen L (2005) Mutatial analysis of the role of calcium ions in the Lactobacillus reurteri strain 121 fructosylçtransferase (levansucrase and inulosucrase) enzymes. FEBS Lett 579:1124-1128
17. Ozimek LK, Kralj S, Kaper T, van der Maarel MJEC, Dijkhuizen L (2006) Single amino acid residue changes in subsite-1 of inulosucrase from Lactobacillus reurteri 121 strongly influence the size of products synthesized. FEBS J 273:4104-4113
18. Risso FVA (2004) Síntese de oligossacarídeos em meio orgânico e aquoso utilizando-se inulinase livre e imobilizada produzida por K. marxianus ATCC 16045 e NRRL Y 7571". Doctorate thesis, Faculty of Food Engineer, UNICAMP, Campinas, Brazil
19. Rubio MC, Runco R, Navarro AR (2002) Invertase from a strain of Rhodotorula glutinis. Phytochemistry 61:605-609
20. Sageetha PT, Ramesh MN, Prapulla SG (2004) Production of fructo-oligosaccharides by fructosyl transferase from Aspergillus orysae CFR202 and Aureobasidium pullulans CFR77. Process Biochem 39:753-758
21. Sageetha PT, Ramesh MN, Prapulla SG (2005) Recent trends in the microbial production, analysis and application of fructooligosaccharides. Trends Food Sci Technol 16:442-457
22. Santos AMP (2003) Sintese de oligossacarideos a partir da sacarose por inulinase de Kluyveromyces marxianus var. bulgaricus". Doctorate thesis, Faculty of Food Engineer, UNICAMP, Campinas, Brazil
23. Somogy M (1945) A new reagent for determination of sugars: a new sugar reagent. May, pp 61-68
24. van Hijum SAFT, Bonting K, van der Maarel MJEC, Dijkhuizen L (2004) Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced. FEMS Microbiol Lett 205:323-328
25. Wang X, Rakshit SK (2000) Iso-oligosaccharide production by multiple forms of transferase enzymes from Aspergillus foetidus. Process Biochem 35:771-775
26. Yoshikawa J, Amachi S, Shinoyama H, Fujii T (2007) Purification and some properties of b-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404. J Biosci Bioeng 103(5):491-493
27. Yun JW (1996) Fructooligosaccharides: occurrence, preparation and application. Enzyme Microb Technol 19:107-117