An on-chip fluorogenic enzyme assay using a multilayer microchip interconnected with a nanocapillary array membrane

IEEE Sensors Journal

Volumn 8, Issue 5, 2008, Pages 601-607

  Gong, Maojun ^a   Kim, Bo Young ^a   Flachsbart, Bruce R ^a   Shannon, Mark A ^a   Bohn, Paul W ^b   Sweedler, Jonathan V ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b University of Notre Dame   (United States)

Author keywords

Enzyme kinetics; Microcontact printing; Microfluidics; Nanocapillary array membranes; Nanofluidics; Poly(methyl methacrylate)

Indexed keywords

CATALYSIS; ENZYME KINETICS; FLUIDIC DEVICES; NANOFLUIDICS; ONLINE SYSTEMS; POLYMETHYL METHACRYLATES;

MICROCONTACT PRINTING; NANOCAPILLARY ARRAY MEMBRANES; ONLINE INCUBATION;

MICROPROCESSOR CHIPS;

EID: 44649151106     PISSN: 1530437X     EISSN: None     Source Type: Journal    
DOI: 10.1109/JSEN.2008.918912     Document Type: Article

References (26)

1. P. S. Dittrich, K. Tachikawa, and A. Manz, "Micro total analysis systems. Latest advancements and trends," Anal. Chem., vol. 78, pp. 3887-3908, 2006.
2. W. Joseph, "On-chip enzymatic assays," Electrophoresis, vol. 23, pp. 713-718, 2002.
3. V. Dolnik and S. Liu, "Applications of capillary electrophoresis on microchip," J. Sep. Sci., vol. 28, pp. 1994-2009, 2005.
4. I. Glasgow and N. Aubry, "Enhancement of microfluidic mixing using time pulsing," Lab Chip, vol. 3, pp. 114-120, 2003.
5. T. C. Kuo, L. A. Sloan, J. V. Sweedler, and P. W. Bohn, "Manipulating molecular transport through nanoporous membranes by control of elec-trokinetic flow: Effect of surface charge density and debye length," Langmuir, vol. 17, pp. 6298-6303, 2001.
6. T.-C. Kuo, D. M. Cannon, Jr, M. A. Shannon, P. W. Bohn, and J. V. Sweedler, "Hybrid three-dimensional nanofluidic/microfluidic devices using molecular gates," Sens. Actuators A, vol. 102, pp. 223-233,2003.
7. M. D. Cannon, Jr., T.-C. Kuo, P. W. Bohn, and J. V. Sweedler, "Nanocapillary array interconnects for gated analyte injections and electrophoretic separations in multilayer microfluidic architectures," Anal. Chem., vol. 75, pp. 2224-2230, 2003.
8. A. N. Chatterjee, D. M. Cannon, E. N. Gatimu, J. V. Sweedler, N. R. Aluru, and P. W. Bohn, "Modeling and simulation of ionic currents in three-dimensional microfluidic devices with nanofluidic interconnects," J. Nanopart. Res., vol. 7, pp. 507-516, 2005.
9. J. S. Kirk, J. V. Sweedler, and P. W. Bohn, "Nanofluidic injection and heterogeneous kinetics of organomercaptan surface displacement reactions on colloidal gold in a microfluidic stream," Anal. Chem., vol. 78, pp. 2335-2341, 2006.
10. J. J. Tulock, M. A. Shannon, P. W. Bohn, and J. V. Sweedler, "Microfluidic separation and gateable fraction collection for mass-limited samples," Anal. Chem., vol. 76, pp. 6419-6425, 2004.
11. T.-C. Kuo, H.-K. Kim, D. M. Cannon, M. A. Shannon, J. V. Sweedler, and P. W. Bohn, "Nanocapillary arrays effect mixing and reaction in multilayer fluidic structures," Angew. Chem. Int. Ed., vol. 43, pp. 1862-1865, 2004.
12. I. H. Chang, J. J. Tulock, J. Liu, W. S. Kim, D. M. Cannon, Y. Lu, P. W. Bohn, J. V. Sweedler, and D. M. Cropek, "Miniaturized lead sensor based on lead-specific DNAzyme in a nanocapillary interconnected microfluidic device," Environ. Sci. Technol., vol. 39, pp. 3756-3761, 2005.
13. B. R. Flachsbart, K. Wong, J. M. Iannacone, E. N. Abante, R. L. Vlach, P. A. Rauchfuss, P. W. Bohn, J. V. Sweedler, and M. A. Shannon, "Design and fabrication of a multilayered polymer microfluidic chip with nanofluidic interconnects via adhesive contact printing," Lab Chip, vol. 6, pp. 667-674, 2006.
14. A. G. Hadd, D. E. Raymond, J. W. Halliwell, S. C. Jacobson, and J. M. Ramsey, "Microchip device for performing enzyme assays," Anal. Chem., vol. 69, pp. 3407-3412, 1997.
15. C. B. Cohen, E. Chin-Dixon, S. Jeong, and T. T. Nikiforov, "A microchip-based enzyme assay for protein kinase A," Anal. Biochem., vol. 273, pp. 89-97, 1999.
16. D. E. Starkey, A. Han, J. J. Bao, C. H. Ahn, K. R. Wehmeyer, M. C. Prenger, H. B. Halsall, and W. R. Heineman, "Fluorogenic assay for ß-glucuronidase using microchip-based capillary electrophoresis," J. Chromatogr. B, vol. 762, pp. 33-41, 2001.
17. B. J. Burke and F. E. Regnier, "Stopped-flow enzyme assays on a chip using a microfabricated mixer," Anal. Chem., vol. 75, pp. 1786-1791, 2003.
18. H. Xu and A. G. Ewing, "A rapid enzyme assay for ß-galactosidase using optically gated sample introduction on a microfabricated chip," Anal. Bioanal. Chem., vol. 378, pp. 1710-1715, 2004.
19. H. Xu and A. G. Ewing, "High-throughput enzyme assay on a multichannel microchip using optically gated sample introduction," Electrophoresis, vol. 26, pp. 4711-4717, 2005.
20. T. V. Zenser, V. M. Lakshmi, and B. B. Davis, "Human and Escherichia coli ß-glucuronidase hydrolysis of glucuronide conjugates of benzidine and 4-aminobiphenyl, and their hydroxy metabolites," DrugMetab. Dispos., vol. 27, pp. 1064-1067, 1999.
21. M. Lorincz, M. Roederer, Z. Diwu, L. A. Herzenberg, and G. P. Nolan, "Enzyme-generated intracellular fluorescence for single-cell reporter gene analysis utilizing Escherichia coli ß-glucuronidase," Cytometry, vol. 24, pp. 321-329, 1996.
22. M. Gong, B. R. Flachsbart, M. A. Shannon, P. W. Bohn, and J. V. Sweedler, "Fluidic communication between multiple vertically segregated microfluidic channels connected by nanocapillary array membranes," Electrophoresis, vol. 29, pp. 1237-1244, 2008.
23. A. M. M. Gaber and B. Ursula, "Manipulation of the electroosmotic flow in glass und PMMA microchips with respect to specific enzymatic glucose determinations," Microchim. Acta, vol. V151, pp. 29-38, 2005.
24. J. Horvath and V. Dolník, "Polymer wall coatings for capillary elec-trophoresis," Electrophoresis, vol. 22, pp. 644-655, 2001.
25. G. J. Mauro, M. M. André, S. T. M. Maria da Glória, F. C. Veríssima, T. E. Mikio, A. Hiroshi, and V. P. L. Onofrio, "Effect of homologous series of n-alkyl sulfates and n-alkyl trimethylammonium bromides on low molecular mass protein tyrosine phosphatase activity," Mol. Cell. Biochem., vol. V265, pp. 133-140, 2004.
26. G. R. Wang, "Laser induced fluorescence photobleaching anemometer for microfluidic devices," Lab Chip, vol. 5, pp. 450-456, 2005.