Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues

Sensors

Volumn 8, Issue 5, 2008, Pages 3106-3122

  Krizkova, Sona ^a   Fabrik, Ivo ^a   Adam, Vojtech ^a   Kukacka, Jiri ^b   Prusa, Richard ^b   Chavis, Grace J ^c   Trnkova, Libuse ^d   Strnadel, Jan ^e   Horak, Vratislav ^e   Kizek, Rene ^a  

^a MENDEL UNIVERSITY IN BRNO   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d MASARYK UNIVERSITY   (Czech Republic)

^e INSTITUTE OF ANIMAL PHYSIOLOGY AND GENETICS   (Czech Republic)

Author keywords

Adsorptive transfer stripping technique; Animal tissue; Brdicka reaction; Cell; Differential pulse voltammetry; Human blood serum; Metallothionein; Protein; Tumour marker

Indexed keywords

ADSORPTIVE TRANSFER STRIPPING; ANIMAL TISSUE; BRDICKA REACTION; DIFFERENTIAL PULSE VOLTAMMETRY; HUMAN BLOODS; METALLOTHIONEINS;

ANIMALS; BLOOD; CELLS; CYTOLOGY; HISTOLOGY; ONCOLOGY; PROTEINS; TUMORS; VOLTAMMETRY;

DERMATOLOGY;

EID: 44649140494     PISSN: 14248220     EISSN: None     Source Type: Journal    
DOI: 10.3390/s8053106     Document Type: Article

References (91)

1. Kagi, J.H.R.; Schaffer, A. Biochemistry of Metallothionein. Biochemistry 1988, 27, 8509-8515.
2. Kizek, R.; Vacek, J.; Adam, V.; Vojtesek, B. Metallothionein - Cisplatin and anticancer therapy. Klin. Biochem. Metab. 2004, 12, 72-78.
3. Studnickova, M.; Turanek, J.; Zabrsova, H.; Krejci, M.; Kysel, M. Rat liver metallothioneins are metal dithiolene clusters. J. Electroanal. Chem. 1997, 421, 25-32.
4. Hamer, D.H. Metallothionein. Annu. Rev. Biochem. 1986, 55, 913-951.
5. Cousins, R.J. Absorption, Transport, and Hepatic-Metabolism of Copper and Zinc - Special Reference to Metallothionein and Ceruloplasmin. Physiol. Rev. 1985, 65, 238-309.
6. Kagi, J.H.R. Overview of Metallothionein. Method Enzymol. 1991, 205, 613-626.
7. Roesijadi, G. Metallothioneins in Metal Regulation and Toxicity in Aquatic Animals. Aquat. Toxicol. 1992, 22, 81-114.
8. Theocharis, S.E.; Margeli, A.P.; Klijanienko, J.T.; Kouraklis, G.P. Metallothionein expression in human neoplasia. Histopathology 2004, 45, 103-118.
9. Dutsch-Wicherek, M.; Popiela, T.J.; Klimek, M.; Rudnicka-Sosin, L; Wicherek, L.; Oudinet, J.P.; Skladzien, J.; Tomaszewska, R. Metallothionein stroma reaction in tumor adjacent healthy tissue in head and neck squamous cell carcinoma and breast adenocarcinoma. Neuroendocrinol. Lett. 2005, 26, 567-574.
10. Klimek, M.; Wicherek, L.; Galazka, K.; Tetlak, T.; Popiela, T.J.; Kulczycka, M.; Rudnicka-Sosin, L.; Dutsch-Wicherek, M. Cycle dependent expression of endometrial metallothionein. Neuroendocrinol. Lett. 2005, 26, 663-666.
11. Klimek, R. Biology of cancer: Thermodynamic answers to some questions. Neuroendocrinol. Lett. 2001, 22, 413-416.
12. Kukacka, J.; Vajtr, D.; Huska, D.; Prusa, R.; Houstava, L.; Samal, F.; Diopan, V.; Kotaska, K.; Kizek, R. Blood Metallothionein, Neuron Specific Enolase, And Protein S100B In Patients With Trauma Brain Injury. Neuroendocrinol. Lett. 2006, 27, 116-120.
13. Prusa, R.; Blastik, O.; Potesil, D.; Trnkova, L.; Zehnalek, J.; Adam, V.; Petrlova, J.; Jelen, F.; Kizek, R. Analytic method for determination of metallothioneins as tumor markers. Clin. Chem. 2005, 51, A56-A56.
14. Prusa, R.; Kizek, R.; Vacek, J.; Trnkova, L.; Zehnalek, J. Study of relationship betwenn metallothionein and heavy metals by CPSA method. Clin. Chem. 2004, 50, A28-A29.
15. Prusa, R.; Petrlova, J.; Kukacka, J.; Adam, V.; Sures, B.; Beklova, M.; Kizek, R. Study of interaction of glutathiones and metallothionein with cytostatics. Clin. Chem. 2006, 52, A175-A175.
16. Prusa, R.; Svoboda, M.; Blastik, O.; Adam, V.; Zitka, O.; Beklova, M.; Eckschlager, T.; Kizek, R. Increase in content of metallothionein as marker of resistence to cisplatin treatment. Clin. Chem. 2006, 52, A174-A175.
17. Dziegiel, P.; Suder, E.; Surowiak, P.; Kornafel, J.; Zabel, M. Expression of metallothionein in synovial sarcoma cells. Appl. Immunohistochem. 2002, 10, 357-362.
18. Goldmann, T.; Moorkamp, A.; Wiedorn, K.H.; Suter, L.; Otto, F. The prognostic value of the expression of collagenase IV, cathepsin D and metallothionein in squamous cell carcinomas of the skin determined by immunohistochemistry. Arch. Dermatol. Res. 2001, 293, 115-120.
19. Goldmann, T.; Ribbert, D.; Suter, L.; Brode, M.; Otto, F. Tumor characteristics involved in the metastatic behaviour as an improvement in primary cutaneous melanoma prognostics. J. Exp. Clin. Cancer Res. 1998, 17, 483-489.
20. Jin, R.X.; Huang, J.X.; Tan, P.H.; Bay, B.H. Clinicopathological significance of metallothioneins in breast cancer. Pathol. Oncol. Res. 2004, 10, 74-79.
21. Mitropoulos, D.; Kyroudi-Voulgari, A.; Theocharis, S.; Serafetinides, E.; Moraitis, E.; Zervas, A.; Kittas, C. Prognostic significance of metallothionein expression in renal cell carcinoma. World J. Gastroenterol. 2005, 3, 5.
22. Ohshio, G.; Imamura, T.; Okada, N.; Wang, Z.H.; Yamaki, K.; Kyogoku, T.; Suwa, H.; Yamabe, H.; Imamura, M. Immunohistochemical study of metallothionein in pancreatic carcinomas. J. Cancer Res. Clin. Oncol. 1996, 122, 351-355.
23. Sauerbrey, A.; Zintl, F.; Volm, M. Expression of Metallothionein in Initial and Relapsed Childhood Acute Lymphoblastic-Leukemia. Ann. Hematol. 1994, 69, 111-115.
24. Shukla, V.K.; Aryya, N.C.; Pitale, A.; Pandey, M.; Dixit, V.K.; Reddy, C.D.; Gautam, A. Metallothionein expression in carcinoma of the gallbladder. Histopathology 1998, 33, 154-157.
25. Sugita, K.; Yamamoto, O.; Asahi, M. Immunohistochemical analysis of metallothionein expression in malignant melanoma in Japanese patients. Am. J. Dermatopathol. 2001, 23, 29-35.
26. Surowiak, P.; Materna, V.; Kaplenko, I.; Spaczynski, M.; Dietel, M.; Lage, H.; Zabel, M. Augmented expression of metallothionein and glutathione S-transferase pi as unfavourable prognostic factors in cisplatin-treated ovarian cancer patients. Virchows Arch. 2005, 447, 626-633.
27. Zelger, B.; Hittmair, A.; Schir, M.; Ofner, C.; Ofner, D.; Fritsch, P.O.; Bocker, W.; Jasani, B.; Schmid, K.W. Immunohistochemically Demonstrated Metallothionein Expression in Malignant-Melanoma. Histopathology 1993, 23, 257-264.
28. Weinlich, G.; Eisendle, K.; Hassler, E.; Baltaci, M.; Fritsch, P.O.; Zeiger, B. Metallothionein - overexpression as a highly significant prognostic factor in melanoma: a prospective study on 1270 patients. Br. J. Cancer 2006, 94, 835-841.
29. Meyskens, F.L.; Farmer, P.J.; Anton-Culver, H. Etiologic pathogenesis of melanoma: A unifying hypothesis for the missing attributable risk. Clin. Cancer Res. 2004, 10, 2581-2583.
30. Weinlich, G.; Bitterlich, W.; Mayr, V.; Fritsch, P.O.; Zelger, B. Metallothionein-overexpression as a prognostic factor for progression and survival in melanoma. A prospective study on 520 patients. Br. J. Dermatol. 2003, 149, 535-541.
31. Sarangarajan, R.; Apte, S.P. The polymerization of melanin: a poorly understood phenomenon with egregious biological implications. Melanoma Res. 2006, 16, 3-10.
32. Happle, R.; Traupe, H.; Vakilzadeh, F.; Macher, E. Arguments in Favor of a Polygenic Inheritance of Precursor Nevi. J. Am. Acad. Dermatol. 1982, 6, 540-542.
33. Kamb, A.; Shattuckeidens, D.; Eeles, R.; Liu, Q.; Gruis, N.A.; Ding, W.; Hussey, C.; Tran, T.; Miki, Y.; Weaverfeldhaus, J.; McClure, M.; Aitken, J.F.; Anderson, D.E.; Bergman, W.; Frants, R.; Goldgar, D.E.; Green, A.; Maclennan, R.; Martin, N.G.; Meyer, L.J.; Youl, P.; Zone, J.J.; Skolnick, M.H.; Cannonalbright, L.A. Analysis of the P16 Gene (Cdkn2) as a Candidate for the Chromosome 9p Melanoma Susceptibility Locus. Nature Genet. 1994, 8, 22-26.
34. Gruis, N.A.; Vandervelden, P.A.; Sandkuijl, L.A.; Prins, D.E.; Weaverfeldhaus, J.; Kamb, A.; Bergman, W.; Frants, R.R. Homozygotes for Cdkn2 (P16) Germline Mutation in Dutch Familial Melanoma Kindreds. Nature Genet. 1995, 10, 351-353.
35. Vasen, H.F.A.; Gruis, N.A.; Frants, R.R.; van der Velden, P.A.; Hille, E.T.M.; Bergman, W. Risk of developing pancreatic cancer in families with familial atypical multiple mole melanoma associated with a specific 19 deletion of p16 (p16-Leiden). Int. J. Cancer 2000, 87, 809-811.
36. Kefford, R.; Bishop, J.N.; Tucker, M.; Bressac-de-Paillerets, B.; Bianchi-Scarra, G.; Bergman, W.; Goldstein, A.; Puig, S.; Mackie, R.; Elder, D.; Hansson, J.; Hayward, N.; Hogg, D.; Olsson, H. Genetic testing for melanoma. Lancet Oncol. 2002, 3, 653-654.
37. Goldstein, A.M.; Chan, M.; Harland, M.; Hayward, N.K.; Demenais, F.; Bishop, D.T.; Azizi, E.; Bergman, W.; Bianchi-Scarra, G.; Bruno, W.; Calista, D.; Albright, L.A.C.; Chaudru, V.; Chompret, A.; Cuellar, F.; Elder, D.E.; Ghiorzo, P.; Gillanders, E.M.; Gruis, N.A.; Hansson, J.; Hogg, D.; Holland, E.A.; Kanetsky, P.A.; Kefford, R.F.; Landi, M.T.; Lang, J.; Leachman, S.A.; MacKie, R.M.; Magnusson, V.; Mann, G.J.; Bishop, J.N.; Palmer, J.M.; Puig, S.; Puig-Butille, J.A.; Stark, M.; Tsao, H.; Tucker, M.A.; Whitaker, L; Yakobson, E. Features associated with germline CDKN2A mutations: a GenoMEL study of melanoma-prone families from three continents. J. Med. Genet. 2007, 44, 99-106.
38. Alvarado, N.E.; Cancio, I.; Hylland, K.; Marigomez, I.; Soto, M. Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd. Histol. Histopath. 2007, 22, 719-728.
39. Alves, S.; Cardoso, S.V.; Bernardes, V.D.F.; Machado, V.C.; Mesquita, R.A.; do Carmo, M.A.V.; Aguiar, M.C.F. Metallothionein immunostaining in adenoid cystic carcinomas of the salivary glands. Oral Oncol. 2007, 43, 252-256.
40. Lobinski, R.; Chassaigne, H.; Szpunar, J. Analysis for metallothioneins using coupled techniques. Talanta 1998, 46, 271-289.
41. Szpunar, J. Bio-inorganic speciation analysis by hyphenated techniques. Analyst 2000, 125, 963-988.
42. Szpunar, J.; Lobinski, R.; Prange, A. Hyphenated techniques for elemental speciation in biological systems. Appl. Spectrosc. 2003, 57, 102A-112A.
43. Chassaigne, H.; Lobinski, R. Characterization of metallothionein isoforms by reversed-phase high-performance liquid chromatography with on-line post-column acidification and electrospray mass spectrometric detection. J. Chromatogr. A 1998, 829, 127-136.
44. Chassaigne, H.; Lobinski, R. Characterization of horse kidney metallothionein isoforms by electrospray MS and reversed-phase HPLC-electrospray MS. Analyst 1998, 123, 2125-2130.
45. Chassaigne, H.; Lobinski, R. Polymorphism and identification of metallothionein isoforms by reversed-phase HPLC with on-line ion spray mass spectrometric detection. Anal. Chem. 1998, 70, 2536-2543.
46. Kizek, R.; Vacek, J.; Trnkova, L; Klejdus, B.; Havel, L. Application of catalytic reactions on a mercury electrode for metallothionein electrochemical detection. Chem. Listy 2004, 98, 166-173.
47. Thompson, J.A.J.; Cosson, R.P. An improved electrochemical method for the quantification of metallothioneins in marine organisms. Marine Environ. Res. 1984, 11, 137-152.
48. Dabrio, M.; Rodriguez, A.R.; Bordin, G.; Bebianno, MJ.; De Ley, M.; Šestakova, I.; Vasak, M.; Nordberg, M. Recent developments in quantification methods for metallothionein. J. Inorg. Biochem. 2002, 88, 123-134.
49. Sestakova, I.; Navratil, T. Voltammetric methods in metallothionein research. Bioinorg. Chem. Appl. 2005, 3, 43-53.
50. Adam, V.; Blastik, O.; Krizkova, S.; Lubal, P.; Kukacka, J.; Prusa, R.; Kizek, R. Application of the Brdicka reaction in determination of metallothionein in patients with tumours. Chem. Listy 2008, 202, 51-58.
51. Adam, V.; Beklova, M.; Pikula, J.; Hubalek, J.; Trnkova, L.; Kizek, R. Shapes of differential pulse voltammograms and level of metallothionein at different animal species. Sensors 2007, 7, 2419-2429.
52. Kukacka, J.; Vajtr, D.; Huska, D.; Prusa, R.; Houstava, L; Samal, F.; Diopan, V.; Kotaska, K.; Kizek, R. Blood metallothionein, neuron specific enolase, and protein S100B in patients with traumatic brain injury. Neuroendocrinol. Lett. 2006, 27, 116-120.
53. Adam, V.; Krizkova, S.; Zitka, O.; Trnkova, L.; Petrlova, J.; Beklova, M.; Kizek, R. Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry. Electroanalysis 2007, 19, 339-347.
54. Petrlova, J.; Potesil, D.; Mikelova, R.; Blastik, O.; Adam, V.; Trnkova, L.; Jelen, F.; Prusa, R.; Kukacka, J.; Kizek, R. Attomole voltammetric determination of metallothionein. Electrochim. Acta 2006, 51, 5112-5119.
55. Yang, M.L.; Zhang, Z.J.; Hu, Z.B.; Li, J.H. Differential pulse anodic Stripping voltammetry detection of metallothionein at bismuth film electrodes. Talanta 2006, 69, 1162-1165.
56. Adam, V.; Petrlova, J.; Potesil, D.; Zehnalek, J.; Sures, B.; Trnkova, L.; Jelen, F.; Kizek, R. Study of metallothionein modified electrode surface behavior in the presence of heavy metal ionsbiosensor. Electroanalysis 2005, 17, 1649-1657.
57. Lopez, M.J.; Arino, C.; Diaz-Cruz, S.; Diaz-Cruz, J.M.; Tauler, R.; Esteban, M. Voltammetry assisted by multivariate analysis as a tool for speciation of metallothioneins: Competitive complexation of alpha- and beta-metallothionein domains with cadmium and zinc. Environ. Sci. Technol. 2003, 37, 5609-5616.
58. Diaz-Cruz, M.S.; Lopez, M.J.; Diaz-Cruz, J.M.; Esteban, M. Comparison of the zinc-cadmium exchange properties of the metallothionein related peptide {Lys-Cys-Thr-Cys-Cys-Ala} and a zinc-containing metallothionein: study by voltammetry and multivariate curve resolution. J. Electroanal. Chem. 2002, 523, 114-125.
59. Sestakova, I.; Mader, P. Voltammetry on mercury and carbon electrodes as a tool for studies of metallothionein interactions with metal ions. Cell. Mol., Biol. 2000, 46, 257-267.
60. Erk, M.; Raspor, B. Advantages and disadvantages of voltammetric method in studying cadmium-metallothionein interactions. Cell. Mol. Biol. 2000, 46, 269-281.
61. Erk, M.; Ivankovic, D.; Raspor, B.; Pavicic, J. Evaluation of different purification procedures for the electrochemical quantification of mussel metallothioneins. Talanta 2002, 57, 1211-1218.
62. Raspor, B. Elucidation of the mechanism of the Brdicka reaction. J. Electroanal. Chem. 2001, 503, 159-162.
63. Brdicka, R. Polarographic investigation in serological cancer diagnosis. Nature 1937, 139, 1020-1021.
64. Brdicka, R. Application of the Polarographic effect of proteins in cancer diagnosis. Nature 1937, 139, 330-330.
65. Huska, D.; Krizkova, S.; Beklova, M.; Havel, L.; Zehnalek, J.; Diopan, V.; Adam, V.; Zeman, L.; Babula, P.; Kizek, R. Influence of cadmium(II) ions and brewery sludge on metallothionein level in earthworms (Eisenia fetida) - Biotransforming of toxic wastes. Sensors 2008, 8, 1039-1047.
66. Kizek, R.; Trnkova, L.; Palecek, E. Determination of metallothionein at the femtomole level by constant current stripping chronopotentiometry. Anal. Chem. 2001, 73, 4801-4807.
67. Kizek, R.; Vacek, J.; Trnkova, L.; Klejdus, B.; Havel, L. Application of catalytic reactions on a mercury electrode for electrochemical detection of metallothioneins. Chem. Listy 2004, 98, 166-173.
68. Krizkova, S.; Zitka, O.; Adam, V.; Beklova, M.; Horna, A.; Svobodova, Z.; Sures, B.; Trnkova, L.; Zeman, L.; Kizek, R. Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues. Czech J. Anim. Sci. 2007, 52, 143-148.
69. Ostatna, V.; Palecek, E. Native, denatured and reduced BSA - Enhancement of chronopotentiometric peak H by guanidinium chloride. Electrochim. Acta 2008, 53, 4014-4021.
70. Petrlova, J.; Krizkova, S.; Zitka, O.; Hubalek, J.; Prusa, R.; Adam, V.; Wang, J.; Beklova, M.; Sures, B.; Kizek, R. Utilizing a chronopotentiometric sensor technique for metallothionein determination in fish tissues and their host parasites. Sens. Actuator B-Chem. 2007, 127, 112-119.
71. Serrano, N.; Sestakova, I.; Diaz-Cruz, J.M. Constant current stripping chronopotentiometry for the study of adsorbing inert and electrochemically nonreversible metal complexes at low concentrations: Application to Cd and Zn metallothioneins. Electroanalysis 2006, 18, 169-176.
72. Serrano, N.; Sestakova, I.; Diaz-Cruz, J.M.; Arino, C. Adsorptive accumulation in constant current stripping chronopotentiometry as an alternative for the electrochemical study of metal complexation by thiol-containing peptides. J. Electroanal. Chem. 2006, 591, 105-117.
73. Sestakova, I.; Kopanica, M.; Havran, L.; Palecek, E. Constant current chronopotentiometric stripping analysis of Cd-metallothionein on carbon and mercury electrodes. Comparison with voltammetry. Electroanalysis 2000, 12, 100-104.
74. Strouhal, M.; Kizek, R.; Vecek, J.; Trnkova, L.; Nemec, M. Electrochemical study of heavy metals and metallothionein in yeast Yarrowia lipolytica. Bioelectrochemistry 2003, 60, 29-36.
75. Tomschik, M.; Havran, L.; Fojta, M.; Palecek, E. Constant current chronopotentiometric stripping analysis of bioactive peptides at mercury and carbon electrodes. Electroanalysis 1998, 10, 403-409.
76. Tomschik, M.; Havran, L.; Palecek, E.; Heyrovsky, M. The "presodium" catalysis of electroreduction of hydrogen ions on mercury electrodes by metallothionein. An investigation by constant current derivative stripping chronopotentiometry. Electroanalysis 2000, 12, 274-279.
77. Trnkova, L.; Kizek, R.; Vacek, J. Catalytic signal of rabbit liver metallothionein on a mercury electrode: a combination of derivative chronopotentiometry with adsorptive transfer stripping. Bioelectrochemistry 2002, 56, 57-61.
78. Lauerova, L.; Dusek, L.; Simickova, M.; Kocak, I.; Vagundova, M.; Zaloudik, J.; Kovarik, J. Malignant melanoma associates with Th1/Th2 imbalance that coincides with disease progression and immunotherapy response. Neoplasma 2002, 49, 159-166.
79. Supalkova, V.; Petrek, J.; Baloun, J.; Adam, V.; Bartusek, K.; Trnkova, L; Beklova, M.; Diopan, V.; Havel, L.; Kizek, R. Multi-instrumental investigation of affecting of early somatic embryos of Spruce by cadmium(II) and lead(II) ions. Sensors 2007, 7, 743-759.
80. Erk, M.; Ivankovi□, D.; Raspor, B.; Pavi□, J. Evaluation of different purification procedures for the electrochemical quantification of mussel metallothioneins. Talanta 2002, 57, 1211-1218.
81. Petrlova, J.; Potesil, D.; Zehnalek, J.; Sures, B.; Adam, V.; Trnkova, L.; Kizek, R. Cisplatin electrochemical biosensor. Electrochim. Acta 2006, 51, 5169-5173.
82. Fortyn, K.; Hruban, V.; Horak, V.; Tichy, J. Exceptional occurrence and extent of malignant melanoma in pig. Vet. Med. 1998, 43, 87-91.
83. Horak, V.; Fortyn, K.; Hruban, V.; Klaudy, J. Hereditary melanoblastoma in miniature pigs and its successful therapy by devitalization technique. Cell. Mol. Biol. 1999, 45, 1119-1129.
84. Borovansky, J.; Horak, V.; Elleder, M.; Fortyn, K.; Smit, N.P.M.; Kolb, A.M. Biochemical characterization of a new melanoma model the minipig MeLiM strain. Melanoma Res. 2003, 13, 543-548.
85. Geffrotin, C.; Crechet, F.; Le Roy, P.; Le Chalony, C.; Leplat, J.J.; Iannuccelli, N.; Barbosa, A.; Renard, C.; Gruand, J.; Milan, D.; Horak, V.; Tricaud, Y.; Bouet, S.; Franck, M.; Frelat, G.; Vincent-Naulleau, S. Identification of five chromosomal regions involved in predisposition to melanoma by genome-wide scan in the MeLiM swine model. Int. J. Cancer 2004, 110, 39-50.
86. Vincent-Naulleau, S.; Le Chalony, C.; Leplat, J.J.; Bouet, S.; Bailly, C.; Spatz, A.; Vielh, P.; Avril, M.F.; Tricaud, Y.; Gruand, J.; Horak, V.; Frelat, G.; Geffrotin, C. Clinical and histopathological characterization of cutaneous melanomas in the melanoblastoma-bearing Libechov minipig model. Pigm. Cell. Res. 2004, 17, 24-35.
87. Svoboda, M.; Eichlerova, K.; Horak, V.; Hradecky, J. Development of haematological indices in melanoma-bearing Libechov Minipigs. Acta Vet. BRNO 2005, 74, 603-611.
88. Le Chalony, C.; Renard, C.; Vincent-Naulleau, S.; Crechet, F.; Leplat, J.J.; Tricaud, Y.; Horak, V.; Gruand, J.; Le Roy, P.; Frelat, G.; Geffrotin, C. CDKN2A region polymorphism and genetic susceptibility to melanoma in the melim swine model of familial melanoma. Int. J. Cancer 2003, 103, 631-635.
89. Geffrotin, C.; Horak, V.; Crechet, F.; Tricaud, Y.; Lethias, C.; Vincent-Naulleau, S.; Vielh, P. Opposite regulation of tenascin-C and tenascin-X in MeLiM swine heritable cutaneous malignant melanoma. Biochim. Biophys. Acta-Gen. Subj. 2000, 1524, 196-202.
90. Potesil, D.; Mikelova, R.; Adam, V.; Kizek, R.; Prusa, R. Change of the protein p53 electrochemical signal according to its structural form - Quick and sensitive distinguishing of native, denatured, and aggregated form of the "guardian of the genome". Protein J. 2006, 25, 23-32.
91. Breazeale, R.I.; Fishburn, J.; Buchanan, T.; Stone, J. Overexpression of metallothionein and survival in uveal melanoma. Invest. Ophthalmol. Vis. Sci. 1996, 37, 2877-2877.