Effects of Sperm Concentration and Straw Volume on Motion Characteristics and Plasma, Acrosomal, and Mitochondrial Membranes of Equine Cryopreserved Spermatozoa

Journal of Equine Veterinary Science

Volumn 28, Issue 6, 2008, Pages 351-358

  Nascimento, J ^a   Raphael, C F ^a   Andrade, A F C ^a   Alonso, M A ^a   Celeghini, E C C ^a   Arruda, R P ^a  

^a Section of Domestic and Wild Animals Anatomy   (Brazil)

Author keywords

Concentration; Cryopreservation; Equine; Spermatozoa; Volume

Indexed keywords

EQUIDAE;

EID: 44649113146     PISSN: 07370806     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jevs.2008.04.010     Document Type: Article

References (56)

1. Neves Neto J.R., Papa F.O., and Leme D.P. Avaliação entre congelabilidade e fertilidade do sêmen eqüino frente a dois diluidores. Rev Bras Reprod Anim 23 (1989) 301-302
2. Marshburn P.B., McIntire D., Carr B.R., and Byrd W. Spermatozoal characteristics from fresh and frozen donor semen and their correlation with fertility outcome after intrauterine insemination. Fertil Steril 58 (1992) 179-186
3. Samper J., and Morris C.A. Current methods for stallion semen cryopreservation: a survey. Theriogenology 49 (1998) 895-903
4. Watson P.F. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci 60-61 (2000) 481-492
5. Blach E.L., Amann R.P., Bowen R.A., and Frantz D. Changes in quality of stallion spermatozoa during cryopreservation plasma membrane integrity and motion characteristics. Theriogenology 31 (1989) 283-298
6. Merkt H., Klug E., Krause D., and Bader H. Results of long-term storage of stallion semen frozen by the pellet method. J Reprod Fertil 23 Suppl (1975) 105-106
7. Suh T.K., Schenk J.L., and Seidel Jr. G.E. High pressure flow cytometric sorting damages sperm. Theriogenology 64 (2005) 1035-1048
8. Love C.C., Loch W.L., Bristol F., Garcia M.C., and Kenney R.M. Comparison of pregnancy rates achieved with frozen semen using two packaging methods. Theriogenology 31 (1989) 613-622
9. Arruda R.P., Vieira R.C., Valente Filho J.B., Visintin J., and Neves Neto J.R. Utilização de uma seringa modificada para aplicação do sêmen eqüino congelado em bainhas plásticas e macrotubos. ARS Vet 10 (1994) 242
10. Loomis P.R., Amann R.P., Squires E.L., and Pickett B.W. Fertility of unfrozen stallion spermatozoa extended in EDTA-lactose-egg yolk and packed in straws. J Anim Sci 56 (1983) 687-693
11. Martin J.C., Klug E., and Guntzel A.R. Centrifugation of stallion semen and its storage in large volume straws. J Reprod Fertil 27 Suppl (1979) 47-51
12. Papa F.O., Prestes N.C., Alvarenga M.A., Bicudo S.D., and Branco M.D.L. Influência de diferentes volumes sobre os parâmetros espermáticos e sobre os índices de fertilidade de sêmen congelado de eqüino. Rev Bras Reprod Anim 1 Suppl (1989) 223
13. Loomis RP, Clark JS. Motion characteristics of frozen-thawed equine spermatozoa packed in 0.5 mL straws at various concentration. Proceedings Society for Theriogenology; 1998:142.
14. Cristanelli M.J., Squires E.L., Amann R.P., and Pickett B.W. Fertility of stallion semen processed, frozen and thawed by a new procedure. Theriogenology 22 (1984) 39-45
15. Heitland A.V., Jasko D.J., Squires E.L., Graham J.K., Pickett B.W., and Hamilton C. Factors affecting motion characteristics of frozen-thawed stallion spermatozoa. Equine Vet J 28 (1996) 47-53
16. thAnnual Convention AAEP; 1992:649-660.
17. Squires E.L., Pickett B.W., Graham J.K., Vanderwall D.K., McCue P.M., and Bruemmer J.E. Factors affecting fertility of frozen-thawed spermatozoa: cooled and frozen stallion semen (1999), Colorado State University Animal Reproduction and Biotechnology Laboratory Bulletin No. 09, Fort Collins, CO 61-77
18. Volkmann D.H., and Van-Zyl D. Fertility of stallion semen frozen in 0.50 mL straws. J Reprod Fertil 35 (1987) 143-148
19. Graham J.K. Cryopreservation of stallion spermatozoa. Vet Clin North Am Equine Pract 12 (1996) 131-147
20. Hammerstedt RH, Drichton EG, Watson PF. Comparative approach to sperm cryopreservation: doses cell shape and size influence cryo-survival? Proceedings Society for Theriogenology; 1991:8-11.
21. Angola A.P., Mendez J.V., and Quintero L.Z. Efecto del sistema de envasado y la concentración espermática sobre el daño acrosomal y la motilidad posdescongelación del semen equino. Vet Méx XXIII 44 (1992) 315-318
22. Buchanan B.R., Seidel Jr. G.E., McCue P.M., Schenk J.L., Herickhoff L.A., and Squires E.L. Insemination of mares with low numbers of either unsexed or sexed spermatozoa. Theriogenology 53 (2000) 1333-1344
23. Lindsey A.C., Bruemmer J.E., and Squires E.L. Low dose insemination of mares using non-sorted and sex-sorted sperm. Anim Reprod Sci 68 (2001) 279-289
24. Petersen M.M., Wessel M.T., Scott M.A., Liu I.K.M., and Ball B.A. Embryo recovery rates in mares after deep intrauterine insemination with low numbers of cryopreserved equine spermatozoa. Theriogenology 58 (2002) 663-665
25. Brinsko S.P., Rigby S.L., Lindsey A.C., Blanchard T.L., Love C.C., and Varner D.D. Pregnancy rates in mares following hysteroscopic or transrectally-guided insemination with low sperm numbers at the utero-tubal papilla. Theriogenology 59 (2003) 1001-1009
26. Manning ST, Bowman PA, Fraser LM, Card CE. Development of hysteroscopic insemination of the uterine tube in the mare. Proceedings Annual Meeting Society for Theriogenology; 1998:84-85.
27. Vazquez JJ, Medina V, Liu IK, Ball BA, Scott MA. Nonsurgical utero-tubal insemination in the mare. Proceedings Annual Meeting Society for Theriogenology; 1998:82-83.
28. Lindsey A.C., Varner D.D., Seidel Jr. G.E., Bruemmer J.E., and Squires E.L. Hysteroscopic or rectally guided deep-uterine insemination of mares with spermatozoa stored 18 h at either 5 degrees C or 15 degrees C prior to flow-cytometric sorting. Anim Reprod Sci 85 (2005) 125-130
29. Verstegen J., Iguer-Ouada M., and Onclin K. Computer assisted semen analyzer in Andrology research and veterinary practice. Theriogenology 57 (2002) 149-179
30. Kavak A., Johannisson N., Lundehein N., Rodriguez-Martinez H., Aidnik M., and Einarsson S. Evaluation of cryopreserved stallion semen from Tori and Estonian breeds using CASA and flow cytometry. Anim Reprod Sci 76 (2003) 205-216
31. Rodriguez-Martinez H. Laboratory semen assessment and prediction of fertility: still utopia?. Reprod Dom Anim 28 (2003) 5-6
32. Kenney RM, Bergman RV, Cooper WL, Morse GW. Minimal contamination techniques for breeding mares and preliminary findings. Proceedings American Association Equine Practictioners; 1975:327-336.
33. Bavister B.D., Leibfried M.L., and Lieberman G. Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol Reprod 28 (1983) 235-247
34. Celeghini ECC, Arruda RP, Andrade AFC, Raphael CF, Nascimento J. Simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa. Proceedings International Congress Animal Reproduction, 2004;15:511.
35. Crockett E.T., Graham J.K., Bruemmer J.E., and Squires E.L. Effect of cooling of equine spermatozoa before freezing on post-thaw motility; preliminary results. Theriogenology 55 (2001) 793-803
36. Blanes M.S., Papa F.O., Dores C.B., Melo C.M., and Crocci A.J. Influência do número de espermatozóides e tempo de estabilização na congelação de sêmen eqüino utilizando-se diluente Botu-Crio. Acta Sci Vet 33 (2005) 303
37. Shivaji S., Peedicayil J., and Devi G.L. Analyses of the motility parameters of in vitro hyperactivated hamster spermatozoa. Mol Reprod Dev 42 (1995) 233-247
38. Jayaprakash D., Kumar S.K., Shivaji S., and Seshagiri P.B. Pentoxifylline induces hyperactivation and acrosome reaction in spermatozoa of golden hamsters: changes in motility kinematics. Hum Reprod 12 (1997) 2192-2199
39. Grondall C., Grondall M.L., Hyttel P., and Greve T. Acrosomal status in fresh and frozen/thawed stallion spermatozoa evaluated by scanning electron microscopy. Anat Embryol 190 (1994) 195-200
40. Amann R.P., and Pickett B.W. Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa. J Equine Vet Sci 7 (1987) 145-173
41. Belous A.M., Luguvoj V.I., Moiseyev V.A., Nardid O.A., and Zagnoyko V.I. Examination by fluorescence and EPR spectroscopy of the state of mitochondrial and lysosomal membranes upon freeze-thawing. Cryobiology 27 (1990) 184-188
42. Neild D.M., Gadella B.M., Chaves M.G., Miragaya M.H., Colenbrander B., and Aguero A. Membrane changes during different stages of a freeze-thaw protocol for equine semen cryopreservation. Theriogenology 59 (2003) 1693-1705
43. Blottner S., Warnke C., Tuchscherer A., Heinen V., and Torner H. Morphological and functional changes of stallion spermatozoa after cryopreservation during breeding and non-breeding season. Anim Reprod Sci 65 (2001) 75-88
44. Schober D., Aurich C., Nohl H., and Gille L. Influence of cryopreservation on mitochondrial functions in equine spermatozoa. Theriogenology 68 (2007) 745-754
45. Pena A., and Linde-Forsberg C. Effects of spermatozoal concentration and post-thaw dilution rate on survival after thawing of dog spermatozoa. Theriogenology 54 (2000) 703-718
46. Celeghini E.C.C., Arruda R.P., Andrade A.F.C., Nascimento J., Raphael C.F., and Rodriguez P.H.M. Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin. Anim Reprod Sci 104 (2008) 119-131
47. Nagy S., Jansen J., Topper E.K., and Gadella B.M. A triple-stain flow cytometric method to assess plasma and acrosome membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod 68 (2003) 1828-1825
48. Martinez-Pastor F., Johannisson A., Gil J., Kaabi M., Anel L., Paz P., et al. Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen. Anim Reprod Sci 84 (2004) 121-133
49. Mukai C., and Okuno M. Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement. Biol Reprod 71 (2004) 540-547
50. Silva P.F.N., and Gadella B.M. Detection of damage in mammalian sperm cells. Theriogenology 65 (2006) 958-978
51. Parks J.E., and Graham J.K. Effects of cryopreservation procedures on sperm membranes. Theriogenology 38 (1992) 209-222
52. Darin-Bennett A., and White I.G. Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology 14 (1977) 466-470
53. Moore A.I., Squires E.L., and Graham J.K. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology 51 (2005) 241-249
54. Squires E.L., Pickett B.W., Graham J.K., Vanderwall D.K., McCue P.M., and Bruemmer J.E. Biology and structure of spermatozoa and their response to cooling. In: Cooled and frozen stallion semen (1999), Colorado State University Animal Reproduction and Biotechnology Laboratory Bulletin No. 09, Fort Collins 3-9
55. Gadella B.M., Rathi R., Brouwers J.F.H.M., Stout T.A.E., and Colenbrander B. Capacitation and the acrosome reaction in equine sperm. Anim Reprod Sci 68 (2001) 249-265
56. Mazur P. Basic concepts in freezing cells. Proceedings First International Conference on Deep Freezing of Boar Semen; 1985:91-111.