Morphometric and biochemical characterization of red beet (Beta vulgaris L.) hairy roots obtained after single and double transformations

Plant Cell Reports

Volumn 27, Issue 6, 2008, Pages 1039-1052

  Thimmaraju, R ^a,b   Venkatachalam, L ^a   Bhagyalakshmi, N ^a  

^a CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

^b UNIVERSITY OF DELAWARE   (United States)

Author keywords

Agrobacterium rhizogenes; Betalaine; Hairy roots; Peroxidase; rol genes; T DNA copy number

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN; BETALAIN; INDOLEACETIC ACID DERIVATIVE; PEROXIDASE; ROL PROTEIN, BACTERIA; T DNA; T-DNA;

ARTICLE; BEET; GENE DOSAGE; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; PLANT ROOT; RHIZOBIUM; TRANSGENIC PLANT;

BACTERIAL PROTEINS; BETA VULGARIS; BETALAINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA, BACTERIAL; GENE DOSAGE; INDOLEACETIC ACIDS; PEROXIDASES; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RHIZOBIUM; TRANSFORMATION, GENETIC;

AGROBACTERIUM RHIZOGENES; BETA VULGARIS; BETA VULGARIS SUBSP. VULGARIS;

EID: 44449088315     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-008-0527-8     Document Type: Article

References (50)

1. Abeysekere M, Sampathu SR, Shankaracharya ML (1990) Studies on different methods of extraction of betalaines from red beet (Beta vulgaris). J Food Sci Technol 27:336-339
2. Agostini E, Milrad de Forchetti S, Tigier HA (1997) Production of peroxidases by hairy roots of Brassica napus. Plant Cell Tissue Organ Cult 47:177-182
3. Agostini E, Hernández-Ruiz J, Arnao MB, Milrad SR, Tigier HA, Acosta MA (2002) Peroxidase isoenzyme secreted by turnip (Brassica napus) hairy-root cultures: inactivation by hydrogen peroxide and application in diagnostic kits. Biotechnol Appl Biochem 35:1-7
4. Bais HP, George J, Ravishankar GA (1999) Influence of polyamines on growth and production of coumarins in hairy root cultures of Cichorium intybus L. cv Lucknow local (Witloof chicory). J Plant Growth Reg 18:33-37
5. Bais HP, Sudha G, George J, Ravishankar GA (2001) Influence of exogenous hormones on growth and secondary metabolite production in hairy root cultures of Cichorium intybus L. cv. Lucknow local. In Vitro Cell Dev Biol Plant 37:293-299
6. Barbier-Brygoo H, Maurel C, Shen WH, Ephritikhine G, Delbarre A, Guern J (1990) Use of mutants and transformed plants to study the action of auxins. In: Roberts JA, Kirk C, Venis M (eds) Hormone perception and signal transduction in animals and plants. Company of Biologists, Cambridge, pp 66-77
7. Bhagyalakshmi N, Thimmaraju R, Narayan MS (2004) Various hexoses and di-hexoses differently influence growth, morphology and pigment synthesis in transformed root cultures of red beet (Beta vulgaris). Plant Cell Tiss Org Cult 78:183-195
8. Bonhomme V, Laurain Mattar D, Fliniaux MA (2000) Effects of the rolC gene on hairy root: induction development and tropane alkaloid production by Atropa belladonna. J Nat Prod 63:1249-1252
9. Bulgakov VP, Khodakovskaya MV, Labetskaya NV, Tchernoded GK, Zhuravlev YN (1998) The impact of plant rolC oncogene on ginsenoside production by ginseng hairy root cultures. Phytochemistry 49:1929-1934
10. Bulgakov VP, Tchernoded GK, Mischenko NP, Shkryl Yu N, Fedoreyev SA, Zhuravlev Yu N (2004) The rolB and rolC genes activate synthesis of anthraquinones in Rubia cordifolia cells by mechanism independent of octadecanoid signaling pathway. Plant Sci 166:1069-1075
11. Camilleri C, Jouanin L (1991) The Tr-DNA region carrying the auxin synthesis genes of the Agrobacterium rhizogenes agropine -type plasmid pRiA4: nucleotide sequence analysis and introduction in to tobacco plants. Mol Plant Microbe Interact 4:155-162
12. Cardarelli M, Mariotti D, Pomponi M, Spano L, Capone I, Constantino P (1987) Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype. Mol Gen Genet 209:475-480
13. Carrizo CN, Pitta-Alvarez SI, Kogan MJ, Giulietti AM, Tomaro ML (2001) Occurrence of cadaverine in hairy roots of Brugmansia candida. Phytochemistry 57:759-763
14. Delbarre P, Muller V, Imhoff H, Barbier-Brygoo C, Maurel N, Lebanc C, Perrot-Rechenmann GJ (1994) The rolB gene of Agrobacterium rhizogenes does not increase the auxin sensitivity of tobacco protoplast by modifying the intracellular auxin concentration. Plant Physiol 105:563-569
15. Doran PM (2002) Properties and applications of hairy root cultures. In: Oksman- Caldentey KM, Barz WH (eds) Plant biotechnology and transgenic plants. Mercel Dekker Inc, New York, pp 143-162
16. Estruch JJ, Chriqui D, Grossman K, Schell J, Spena A (1991a) The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates. EMBO J 10:2889-2895
17. Estruch JJ, Schell J, Spena A (1991b) The protein encoded by the rolB plant oncogene hydrolyses indole glucosides. EMBO J 10:3125-3128
18. Faiss M, Strnad M, Redig P, Doležal K, Hanuš J, Van Onckelen H, Schmülling T (1996) Chemically induced expression of the rolC-encoded β-glucosidase in transgenic tobacco plants and analysis of cytokinin metabolism: rolC does not hydrolyze endogenous cytokinin glucosides in planta. Plant J 10:33-46
19. Harter LN (1960) Critical values for Duncan's new multiple range test. Biometrics 16:671-685
20. Inze D, Follin A, van Lijsebettens M (1984) Genetic analysis of individual T-DNA genes of Agrobacterium tumefaciens; further evidence that two genes are involved in indole-3-acetic acid synthesis. Mol Gen Genet 194:265-274
21. Knegt E, Bruinsma J (1973) Rapid sensitive and accurate determination of indolyl -3-acetic acid. Phytochemistry 12:753
22. Lacroix B, Tzfira T, Vainstein A, Citovsky V (2006) A case of promiscuity: Agrobacterium's endless hunt for new partners. Trends Genet 22:29-37
23. Lagrimini LM (1996) The role of the tobacco anionic peroxidase in growth and development. In: Obinger C, Burner U, Ebermann R, Penel C, Greppin H (eds) Plant peroxidases, biochemistry and physiology. University of Geneva, Geneva, pp 235-242
24. L-DNA that alter plant morphogenesis or hormone sensitivity. Plant J 15:423-434
25. Liu ZH, Liu HY, Wang HY (1996) Effect of light on endogenous indole-3-acetic acid, peroxidase and Indole-3-acetic acid oxidase in soybean hypocotyls. Bot Bull Acad Sin 37:113-119
26. Maurel S, Barbier-Bryqoo H, Spena A, Tempe G, Guern G (1991) Single rol genes from the Agrobacterium rhizogenes T(L)-DNA alter some of the cellular responses to auxin in Nicotiana tobacum. Plant Physiol 97:212-216
27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:473-497
28. Nilsson T (1970) Studies in to the pigments in beet root (Beta vulgaris L. sp. vulgaris var. rubra L.). Lantbrukshogsk Ann 36:179-219
29. Palazón J, Cusidó RM, Roig C, Piñol MT (1998a) Expression of the rolC gene and nicotine production in transgenic roots and their regenerated plants. Plant Cell Rep 17:384-390
30. Palazón J, Cusidó RM, Gonzalo J, Bonfill M, Morales S, Piñol MT (1998b) Relation between the amount the rolC gene product and indole alkaloid accumulation in Catharantus roseus transformed root cultures. J Plant Physiol 153:712-718
31. Prabha TN, Bhagyalakshmi N (1998) Carbohydrate metabolism in ripening banana fruit. Phytochemistry 48:915-919
32. Pressey R (1990) Anions activate the oxidation of indoleacetic acid by peroxidases from tomato and other sources. Plant Physiol 93:798-804
33. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. In: Ford N, Nolan C, Ferguson M, Ockler M (eds) Cold Spring Harbor laboratory Press, New York, pp 1.3-7.8
34. Savitha BC, Thimmaraju R, Bhagyalakshmi N, Ravishankar GA (2006) Different biotic and abiotic elicitors influence betalaine production in hairy root cultures of Beta vulgaris in shake flask and bioreactor. Process Biochem 41:50-60
35. Schmulling T, Schell J, Spena A (1988) Single genes from Agrobacterium rhizogenes influence plant development. EMBO J 7:2621-2629
36. Schmulling T, Schell J, Spena A (1989) Promoters of the rolA, B and C genes of Agrobacterium rhizogenes are differentially regulated in transgenic plants. Plant Cell 1:665-670
37. Schmulling T, Fladung M, Grossman K, Shell J (1993) Hormonal content and sensitivity of transgenic tobacco and potato plants expressing single rol genes of A. rhizogenes T-DNA. Plant J 3:371-382
38. Sedira M, Butler E, Gallagherb T, Welandera M (2005) Verification of auxin-induced gene expression during adventitious rooting in rolB-transformed and untransformed apple. Plant Sci 168:1193-1198
39. Shanks JV, Bhadra R (1997) Characteristics of selected hairy root lines of Catharanthus roseus. In: Doran PM (ed) Hairy roots: culture and applications. Harwood academic Publishers, pp 51-65
40. Shkryl YN, Veremeichikl NG, Bulgakov PV, Tchernoded GK, Mischenko NP, Fedoreyev SA, Zhuravlev YN (2008) Individual and combined effects of the rolA, B and C genes on anthraquinone production in Rubia cordifolia transformed calli. Biotechnol Bioeng. doi: 10.1002/bit. 21727
41. Stossel A, Venis MA (1970) Determination of sub-microgram levels of indole-3-acetic acid: a new highly specific method. Anal Biochem 34:344
42. Spena A, Schmülling T, Koncz C, Schell JS (1987) Independent and synergistic activity of rol A, B and C loci in stimulating abnormal growth in plants. EMBO J 6:3891-3899
43. Sudha CG, Obul Reddy B, Ravishankar GA, Seeni S (2003) Production of ajmalicine and ajmaline in hairy root cultures of Rauvolfia micrantha Hook, a rare and endemic medicinal plant. Biotechnol Lett 25:631-636
44. Tanaka N, Fuzikawa Y, Aly MAM, Sanoeka H, Fujita K, Yamashita I (2001) Proliferation and rol gene expression in hairy root lines of Egyptian clover. Plant Cell Tissue Organ Cult 66:175-182
45. Thimmaraju R, Bhagyalakshmi N, Ravishankar GA (2004) In situ and ex situ adsorption and recovery of betalaines from hairy root cultures of Beta vulgaris. Biotechnol Prog 20:777-785
46. Thimmaraju R, Bhagyalaskshmi N, Narayan MS, Ravishankar GA (2003a) Kinetics of pigment release from hairy root cultures of red beet under the influence of pH, sonication, temperature and oxygen stress. Process Biochem 38:1067-1074
47. Thimmaraju R, Bhagyalaskshmi N, Narayan MS, Ravishankar GA (2003b) Food grade chemical and biological agents permeabilize red beet hairy roots assisting the release of betalaines. Biotechnol Prog 19:1274-1282
48. Thimmaraju R, Vinod Kumar, Venkatachalam L, Bhagyalakshmi N, Ravishankar GA (2005) Peroxidase production from hairy root cultures of red beet (Beta vulgaris). Electron J Biotechnol 8:185-196
49. Wang JW, Tan RX (2002) Artemisin production in Artemisia annua hairy root cultures with improved growth by altering the nitrogen source in the medium. Biotechnol Lett 24:1153-1156
50. Zhang L, Ding R, Chai Y, Bonfill M, Moyano E, Oksman-Caldentey KM, Xu T, Pi Y, Wang Z, Zhang H, Kai G, Liao Z, Sun X, Tang K (2004) Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. Proc Natl Acad Sci USA 101:6786-6791