An attempt to render oocytes and embryos free from the porcine circovirus type 2 after experimental in vitro exposure

Canadian Journal of Veterinary Research

Volumn 68, Issue 3, 2004, Pages 222-225

  Bielanski, Andrzej ^a   Larochelle, Renée ^b   Magar, Ronald ^b  

^a CANADIAN FOOD INSPECTION AGENCY   (Canada)

^b Saint Hyacinthe Laboratory   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEASE; ENZYME; HYALURONIDASE; NUCLEIC ACID; PRONASE; RIBONUCLEASE; TRYPSIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EMBRYO; ANIMAL TISSUE; ARTICLE; BREEDING; CIRCOVIRIDAE; CUMULUS OOPHORUS; DISINFECTION; EMBRYO; EMBRYO CELL; FEMALE; FERTILIZATION IN VITRO; IN VITRO STUDY; NONHUMAN; OOCYTE; VIRAL CONTAMINATION;

ANIMALS; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; DNA PRIMERS; DNA, VIRAL; EMBRYO; FEMALE; FERTILIZATION IN VITRO; IRRIGATION; OOCYTES; POLYMERASE CHAIN REACTION; SWINE; SWINE DISEASES;

ANIMALIA; CIRCOVIRIDAE; CIRCOVIRUS; PORCINE CIRCOVIRUS; PORCINE CIRCOVIRUS 2; SUIDAE;

EID: 4444382417     PISSN: 08309000     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Singh EL. Disease control potential of embryos. Theriogenology 1987;27:9-20.
2. Tischer I, Gelderblom H, Vettermann W, Koch MA. A very small porcine virus with circular single-stranded DNA. Nature 1982;295:64-66.
3. Tischer I, Mields W, Wolff D, Vagt M, Griem W. Studies on epidemiology and pathogenicity of porcine circovirus. Arch Virol 1986;91:271-276.
4. Ellis J, Hassard L, Clark E, et al. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can Vet J 1998;39:44-51.
5. Allan GM, Ellis JA. Porcine circoviruses: a review. J Vet Diag Invest 2000; 12:3-14.
6. Segales J, Domingo M. Postweaning multisystemic wasting syndrome (PMWS) in pigs. A review. Vet Q 2002;24:109-124.
7. Mateusen B, Sanchez RE, Van Soom A, Meerts P, Maes DGD, Nauwynck HJ. Susceptibility of pig embryos to porcine circovirus type 2 infection. Theriogenology 2004;61:91-101.
8. Larochelle R, Morin M, Antaya M, Magar R. Identification and incidence of porcine circovirus in routine field cases in Québec as determined by PCR. Vet Record 1999;145: 140-142.
9. Stringfellow DA, Seidel S. Manual of the International Embryo Transfer Society. International Embryo Transfer Society (IETS), Urbana, Illinois, USA. 1998.
10. Magar R, Müller P, Larochelle R. Retrospective serologic survey of antibodies to porcine circovirus type 1 and type 2. Can J Vet Res 2000;64:184-186.
11. Petters RM, Wells KD. Culture of pig embryos. J Reprod Fertil Suppl 1993;48:61-73.
12. Larochelle R, Bielanski A, Muller P, Magar R. PCR detection and evidence of shedding of porcine circovirus type 2 in boar semen. J Clin Microbiol 2000;38:4629-4632.
13. Bielanski A, Hare WC. Investigation of some antimicrobial procedures on the in vitro development of early murine embryos aimed toward developing methods for the disinfection of mammalian embryos prior to transfer. J In Vitro Fert Embryo Transf 1991;8:24-32.
14. Anon. International Animal Health Code. Office International des Épizooties, Paris, France. Appendix 3.3.4. Porcine embryos/ova. 2002:366-370.
15. Bielanski A, Dubuc C. In vitro fertilization and culture of ova from heifers infected with bovine herpesvirus-1 (BHV-1). Theriogenology 1994;41:211-1217.
16. Talbot P. Hyaluronidase dissolves a component in the hamster zona pellucida. J Exp Zool 1984;229:309-316.
17. Bureau M, Dea S, Sirard MA. Evaluation of virus decontamination techniques for porcine embryos produced in vitro (Abstract). Proc Society for Study of Reproduction, Ottawa, Ontario. 2001;514:308.
18. Bielanski A, Dubuc C, Hare WCD. Inactivation of bovine herpesvirus-1 and bovine virus diarrhoea virus in association with preimplantation bovine embryos using photosensitive agents. Theriogenology 1992;38:633-644.