Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography

Methods in Molecular Biology

Volumn 94, Issue , 1999, Pages 235-242

  Biersack, Harald ^a   Jensen, Sanne ^b   Westergaard, Ole ^c  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c AARHUS UNIVERSITY   (Denmark)

Author keywords

Bead Beater; FPLC System; Metal Chelate Affinity Chromatography; Nickel Column; Triose Phosphate Isomerase

Indexed keywords

DNA TOPOISOMERASE (ATP HYDROLYSING); GLUTATHIONE TRANSFERASE; HISTIDINE; HYBRID PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY;

CHROMATOGRAPHY, AFFINITY; DNA TOPOISOMERASES, TYPE II; GLUTATHIONE TRANSFERASE; HISTIDINE; RECOMBINANT FUSION PROTEINS;

EID: 4444380944     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1385/1-59259-259-7:235     Document Type: Chapter

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