Targeting of Bacillus anthracis interaction factors for human macrophages using two-dimensional gel electrophoresis

Biochemical and Biophysical Research Communications

Volumn 322, Issue 3, 2004, Pages 854-859

  Seo, Gwi Moon ^a   Kim, Seong Joo ^a   Kim, Ji Chon ^a   Nam, Deok Hwa ^a   Yoon, Moon Young ^b   Koo, Bon Sung ^c   Chai, Young Gyu ^a  

^a Hanyang University   (South Korea)

^b Hanyang University   (South Korea)

^c National Institute of Animal Science   (South Korea)

Author keywords

Bacillus anthracis; Human macrophages; Interaction; Mass spectroscopy; Spores; Two dimensional gel electrophoresis

Indexed keywords

ANTHRAX; APOPTOSIS; ARTICLE; BACILLUS ANTHRACIS; BACTERIAL SPORE; BACTERIAL VIRULENCE; CELL DIVISION; CONTROLLED STUDY; CYTOSKELETON; HUMAN; HUMAN CELL; MACROPHAGE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FUNCTION; PROTEOMICS; REGULATORY MECHANISM; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BACILLUS ANTHRACIS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; MACROPHAGES; PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPORES, BACTERIAL;

BACILLI; BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 4444350477     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.07.190     Document Type: Article

References (19)

1. C. Guidi-Rontani The alveolar macrophage: the Trojan horse of Bacillus anthracis Trends Microbiol. 10 2002 405 409
2. D.C. Dragon, and R.P. Rennie Evaluation of spore extraction and purification methods for selective recovery of viable Bacillus anthracis spores Lett. Appl. Microbiol. 33 2001 100 105
3. C. Guidi-Rontani, M. Weber-Levy, E. Labruyere, and M. Mock Germination of Bacillus anthracis spores within alveolar macrophage Mol. Microbiol. 31 1999 9 17
4. P.C. Hanna, S. Kochi, and R.J. Collier Biochemical and physiological changes induced by anthrax lethal toxin in J774 macrophage-like cells Mol. Biol. Cell 3 1992 1269 1277
5. S.G. Popov, R. Villasmil, J. Bernardi, E. Grene, J. Cardwell, and T. Popova Effect of Bacillus anthracis lethal toxin on human peripheral blood mononuclear cells FEMS Lett. 527 2002 211 215
6. G. Tang, and S.H. Leppla Proteasome activity is required for anthrax lethal toxin to kill macrophages Infect. Immun. 67 1999 3055 3060
7. J. Garin, R. Diez, S. Kieffer, J.F. Dermine, S. Duclos, E. Gagnon, R. Sadoul, C. Rondeau, and M. Desjardins The phagosome proteome: insight into phagosome function J. Cell Biol. 152 2001 165 180
8. N. Lee, H. MacDonald, C. Reinhard, R. Halenbeck, A. Roulston, T. Shi, and L.T. Williams Activation of hPAK65 by caspase cleavage induces some of the morphological and biochemical changes of apoptosis Proc. Natl. Acad. Sci. USA 94 1997 13642 13647
9. D.S. Black, and J.B. Bliski The RhoGAP activity of the Yersinia pseudotuberculosis cytotoxin YopE is required for antiphagocytic function and virulence Mol. Microbiol. 37 2000 515 527
10. K. Funato, W. Beron, C.Z. Yang, A. Mukhopadhyay, and P.D. Stahl Reconstitution of phagosome-lysosome fusion in streptolysin O-permeabilized cells J. Biol. Chem. 272 1997 16147 16151
11. R.E. Molestina, J.B. Klein, R.D. Miller, W.H. Pierce, J.A. Ramirez, and J.T. Summersgill Proteomic analysis of differentially expressed Chlamydia pneumoniae genes during persistent infection of HEp-2 cells Infect. Immun. 6 2002 2976 2981
12. J.T. Regula, B. Ueberle, G. Boguth, A. Görg, M. Schnölzer, R. Herrmann, and R. Frank Towards a two-dimensional proteome map of Mycoplasma pneumonia Electrophoresis 21 2000 3765 3780
13. T.C. Dixon, A.A. Fadl, T.M. Koehler, J.A. Swanson, and P.C. Hanna Early Bacillus anthracis-macrophage interactions: intracellular survival and escape Cell Microbiol. 6 1999 453 463
14. S.J. Kim, K.S. Cho, Y.K. Choi, and Y.G. Chai Molecular Diversity of pagA Gene from Bacillus anthracis Korean J. Microbiol. 37 2001 49 55
15. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding Anal. Biochem. 72 1976 248 254
16. B.A. Oakley, D.R. Kirsch, and N.R. Morris A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels Anal. Biochem. 105 1980 361 363
17. A. Shevchenko, M. Wilm, O. Vorm, and M. Mann Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels Anal. Biochem. 68 1996 850 858
18. B.D. Green, L. Battisti, T.M. Koehler, C.B. Thorne, and B.E. Ivins Demonstration of a capsule plasmid in Bacillus anthracis Infect. Immun. 49 1985 291 297
19. A.M. Weissman Regulating protein degradation by ubiquitination Immunol. Today 18 1997 189 198