Implementation of HPLC methodology for the quantification of malondialdehyde in cell suspensions and liver

Journal of Liquid Chromatography and Related Technologies

Volumn 27, Issue 15, 2004, Pages 2357-2369

  Soares, M Elisa ^a   Carvalho, Márcia ^a   Remião, Fernando ^a   Carvalho, Félix ^a   Bastos, Maria De Lourdes ^a  

^a UNIVERSITY OF PORTO   (Portugal)

Author keywords

Cell suspensions; HPLC UV; Liver; Malondialdehyde

Indexed keywords

BIOMARKERS; CHROMATOGRAPHIC ANALYSIS; DISEASE CONTROL; HEALTH CARE; SUSPENSIONS (FLUIDS); TEMPERATURE DISTRIBUTION;

CELL SUSPENSIONS; HUMAN SAMPLES; PLASMA SAMPLES;

LIQUID CHROMATOGRAPHY;

BIOLOGICAL MARKER; MALONALDEHYDE; THIOBARBITURIC ACID;

ACCURACY; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL SUSPENSION; CHROMATOGRAPHY; COMPLEX FORMATION; CONTROLLED STUDY; ENVIRONMENTAL TEMPERATURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIPID PEROXIDATION; LIVER; LIVER CELL; NONHUMAN; OXIDATIVE STRESS; PLASMA; RAT; RELIABILITY; SERUM; URINE; VALIDATION PROCESS;

ANIMALIA;

EID: 4444331498     PISSN: 10826076     EISSN: None     Source Type: Journal    
DOI: 10.1081/JLC-200028139     Document Type: Article

References (12)

1. Marnett, L.J. Oxy radicals, lipid peroxidation and DNA damage. Toxicology 2002, 181/182, 219-222.
2. Ohya, T. Reactivity of alkanals towards malondialdehyde (MDA) and the effect of alkanals on MDA determination with a thiobarbituric acid test. Biol. Pharm. Bull. 1993, 16, 1078-1082.
3. Lefevre, G.; Beljean-Leymarie, M.; Beyerle, F.; Bonnefont-Rousselot, D.; Cristol, J.P.; Therond, P.; Torreilles, J. Evaluation of lipid peroxidation by measuring thiobarbituric acid reactive substances. Ann. Biol. Clin. (Paris) 1998, 56, 305-319.
4. Roman, K.; Vladimira, M.; Alexander, C. Highly specific, simple and rapid method for the determination of malondialdeyde in blood using high-performance liquid chromatography. Clin. Chem. Lab. Med. 2002, 40, 1032-1035.
5. Karatas, F.; Karatepe, M.; Baysar, A. Determination of free malondialdehyde in human serum by high-performance liquid chromatography. Anal. Biochem. 2002, 311, 76-79.
6. Templar, J.; Kon, S.P.; Milligan, T.P.; Newman, D.J.; Raftery, M.J. Increased plasma malondialdehyde levels in glomerular disease as determined by a fully validated HPLC method. Nephrol. Dial. Transplant. 1999, 14, 946-951.
7. Sim, A.S.; Salonikas, C.; Naidoo, D.; Wilcken, D.E.L. Improved method for plasma malondialdeyde measurement by high-performance liquid chromatography using methyl malondialdehyde as an internal standard. J. Chromatogr. B 2003, 785, 337-344.
8. Korchazhkina, O.; Exley, C.; Spencer, S.A. Measurement by reversed-phase high-performance liquid chromatography of malondialdehyde in normal human urine following derivatisation with 2,4-dinitrophenylhydrazine. J. Chromatogr. B 2003, 794, 353-362.
9. Sheu, J.-Y.; Ku, H.-P.; Tseng, W.-C.; Chen, M.-T.; Tsai, L.-Y.; Huang, Y.-L. Determination of thiobarbituric acid adduct of malondialdehyde using on-line microdialysis coupled with high-performance liquid chromatography. Anal. Sci. 2003, 19, 621-624.
10. Yagi, K. A simple fluorometric assay for lipid peroxides in blood serum or plasma. In CRC Handbook of Free Radicals and Antioxidants in Biomedicine; Miquel, J., Ed.; CRC Press: Boca Raton, 1989; 215-217.
11. Carvalho, M.; Milhazes, N.; Remião, F.; Borges, F.; Fernandes, E.; Amado, F.; Monks, T.; Carvalho, F.; Bastos, M.L. Hepatotoxicity of 3,4-methylenedioxyamphetamine and alpha-methyldopamine in isolated rat hepatocytes: formation of glutathione conjugates. Arch. Toxicol. 2004, 78, 16-24.
12. Fernandes, E.; Carvalho, M.; Carvalho, F.; Silva, A.M.S.; Santos, C.M.M.; Pinto, D.C.G.A.; Cavaleiro, J.A.S.; Bastos, M.L. Hepatoprotective activity of polyhydroxylated 2-styrylchromones against tert-butylhydroperoxide-induced toxicity in freshly isolated rat hepatocytes. Arch. Toxicol. 2003, 77, 500-505.