A novel ELISpot method for adherent cells

Journal of Immunological Methods

Volumn 291, Issue 1-2, 2004, Pages 63-70

  Turner, Chantal K ^a   Blieden, Timothy M ^b   Smith, Terry J ^c   Feldon, Steven E ^d   Foster, David C ^a   Sime, Patricia J ^a,d   Phipps, Richard P ^a,d,e  

^a UNIVERSITY OF ROCHESTER   (United States)

^b UNIVERSITY OF ROCHESTER   (United States)

^c HARBOR UCLA MEDICAL CENTER   (United States)

^d UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^e UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

nitrophenylphosphate; NPP; Adherent Cells; bovine serum albumin; BSA; Cytokine; ELISA; ELISpot; enzyme linked immunosorbent assay; FBS; fetal bovine serum; Fibroblast; IL; interleukin; MEM; Modified Eagle's Medium; PBS; phosphate buffered saline

Indexed keywords

INTERLEUKIN 1BETA; INTERLEUKIN 6; INTERLEUKIN 8;

ADHERENT CELL; ARTICLE; CELL ADHESION; CELL CULTURE; CELL STIMULATION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME LINKED IMMUNOSPOT ASSAY; FIBROBLAST; GINGIVA; HUMAN; HUMAN CELL; HUMAN TISSUE; INCUBATION TIME; LUNG; LYMPHOCYTE; MYOMETRIUM; ORBIT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION;

CELL ADHESION; CELLS, CULTURED; ENZYME-LINKED IMMUNOSORBENT ASSAY; FIBROBLASTS; HUMANS; IMMUNOENZYME TECHNIQUES; INTERLEUKIN-6; INTERLEUKIN-8; SENSITIVITY AND SPECIFICITY; TIME FACTORS; TITRIMETRY;

BOVINAE; CICONIIFORMES;

EID: 4444318832     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.04.023     Document Type: Article

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