Recombinant expression of Ole e 6, a Cys-enriched pollen allergen, in Pichia pastoris yeast: Detection of partial oxidation of methionine by NMR

Protein Expression and Purification

Volumn 37, Issue 2, 2004, Pages 336-343

  Barral, Patricia ^a   Tejera, María Luisa ^a   Treviño, Miguel Ángel ^b   Batanero, Eva ^a   Villalba, Mayte ^a   Bruix, Marta ^b   Rodríguez, Rosalía ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b INSTITUTO DE QUÍMICA FÍSICA ROCASOLANO   (Spain)

Author keywords

Allergen; NMR; Olive pollen; Pichia pastoris

Indexed keywords

IMMUNOGLOBULIN G; METHIONINE; OLE6 PROTEIN, OLEA EUROPAEA; OXYGEN; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CHROMATOGRAPHY; CIRCULAR DICHROISM; GENE VECTOR; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PICHIA; POLLEN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; TIME; ULTRAVIOLET RADIATION;

CHROMATOGRAPHY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENETIC VECTORS; IMMUNOGLOBULIN G; MAGNETIC RESONANCE SPECTROSCOPY; METHIONINE; OXYGEN; PICHIA; PLANT PROTEINS; POLLEN; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; TIME FACTORS; ULTRAVIOLET RAYS;

PICHIA; PICHIA PASTORIS;

EID: 4444290944     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.06.012     Document Type: Article

References (24)

1. Kay A.B. Concepts of allergy and hypersensitivity. Kay A.B. Allergy and Allergic Diseases. 1997;24 Blackwell Publications, Oxford
2. Bousquet J., Cour P., Guerin B., Michel F.-B. Allergy in Mediterranean area. I. Pollen counts and pollinosis in Montpellier. Clin. Allergy. 14:1985;249-258
3. Macchia L., Caiaffa M.F., D'Amato G., Tursi A. Allergenic significance of Oleaceae pollen. D'Amato G., Spieksma F.Th.M., Bonini S. Allergic Pollen and Pollinosis in Europe. 1991;87-93 Blackwell Scientific Publications, Oxford
4. Rodríguez R., Villalba M., Monsalve R.I., Batanero E. The spectrum of olive pollen allergens. Int. Arch. Allergy Immunol. 125:2001;185-195
5. Huecas S., Villalba M., Rodríguez R. Ole e 9, a major olive pollen allergen is a 1,3-β-glucanase. J. Biol. Chem. 276:2001;27959-27966
6. Barral P., Batanero E., Palomares O., Quiralte J., Villalba M., Rodríguez R. A major allergen from pollen defines a novel family of plant proteins and shows intra and interspecie cross-reactivity. J. Immunol. 172:2004;3644-3651
7. Batanero E., Ledesma A., Villalba M., Rodríguez R. Purification, amino acids sequence and immunological characterization of Ole e 6, a cysteine-enriched allergen from olive tree pollen. FEBS Lett. 410:1997;293-296
8. Valenta R., Kraft D. From allergen structure to new forms of allergen-specific immunotherapy. Curr. Opin. Immunol. 14:2002;718-727
9. Chapman M.D., Smith A.M., Vailes L.D., Arruda L.K., Dhanaraj V., Pomes A. Recombinant allergens for diagnosis and therapy of allergic disease. J. Allergy Clin. Immunol. 106:2000;409-418
10. White C.E., Kempi N.M., Komives E.A. Expression of highly disulphide-bonded proteins in Pichia pastoris. Structure. 2:1994;1003-1005
11. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain termination inhibitors. Proc. Natl. Acad. Sci. USA. 74:1977;5460-5467
12. Gietz R.D., Schiestl R.H. Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier. Yeast. 7:1991;253-263
13. Perczel A., Hollosi M., Tusnady G., Fasman G.D. Convex constrain analysis: a natural deconvolution of circular dichroism curves of proteins. Protein Eng. 4:1991;669-679
14. 15N chemical shift referencing in biomolecular NMR. J. Biomol. NMR. 6:1995;135-140
15. Delaglio F., Grzesiek S., Vuister G.W., Zhu G., Pfeifer J., Bax A. NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J. Biomol. NMR. 6:1995;277-293
16. Helgstrand M., Kraulis P., Allard P., Hard T. Ansig for Windows: an interactive computer program for semiautomatic assignment of protein NMR spectra. J. Biomol. NMR. 18:2000;29-336
17. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685
18. Villalba M., Batanero E., Monsalve R.I., Gónzález de la Peña M.A., Lahoz C., Rodríguez R. Cloning and expression of Ole e 1, the major allergen from olive tree pollen. J. Biol. Chem. 269:1994;15217-15222
19. Batanero E., Villalba M., Ledesma A., Puente X.S., Rodríguez R. Ole e 3, an olive-tree allergen, belongs to a widespread family of pollen proteins. Eur. J. Biochem. 241:1996;772-778
20. K. Wütrich, NMR of Proteins and Nucleic Acids, Wiley (Eds.), New York, 1986
21. 15N random coil NMR chemical shifts of the common amino acids. I. Investigations of nearest-neighbour effects. J. Biomol. NMR. 5:1995;67-81
22. E. Pretsch, P. Bühlmann, C. Affolter, A. Herrera, A. Martínez, Structure Determination of Organic Compounds, Springer-Verlag (Eds.), Berlin, 2000
23. Shapiro R.I., Wen D., Levesque M., Hronowski X., Gill A., Garber E.A., Galdes A., Strauch K.L., Taylor F.R. Expression of Sonic hedgehog-Fc fusion protein in Pichia pastoris. Identification and control of post-translational, chemical, and proteolytic modifications. Protein Expr. Purif. 29:2003;272-283
24. Stadtman E.R., Moskovitz J., Berlett B., Levine S.R.L. Cyclic oxidation and reduction of protein methionine residues is an important antioxidant mechanism. Mol. Cell. Biochem. 234-235(1-2):2002;3-9