Identification of binding epitope of a monoclonal antibody (Z12) against human TNF-α using computer modeling and deletion mutant technique

Science in China, Series C: Life Sciences

Volumn 47, Issue 3, 2004, Pages 279-286

  Zhang, Wei ^a   Feng, Jiannan ^a   Shen, Beifen ^a  

^a INSTITUTE OF BASIC MEDICAL SCIENCES   (China)

Author keywords

Antigenic epitope; Deletion; Fv; Homology based modeling; Human tumor necrosis factor

Indexed keywords

AMINO ACIDS; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; BIOTECHNOLOGY; MUTAGENESIS; NATURAL SCIENCES;

ANTIGENIC EPITOPE; DELETION; FV FRAGMENT; HOMOLOGY BASED MODELING; HUMAN TUMOR NECROSIS FACTOR;

MONOCLONAL ANTIBODIES;

PROKARYOTA;

EPITOPE; IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY; PEPTIDE FRAGMENT; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL; ANTIGEN ANTIBODY COMPLEX; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE DELETION; GENETICS; HUMAN; HYDROGEN BOND; IMMUNOLOGY; MOUSE; PROTEIN TERTIARY STRUCTURE;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGEN-ANTIBODY COMPLEX; COMPUTER SIMULATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; HUMANS; HYDROGEN BONDING; IMMUNOGLOBULIN FAB FRAGMENTS; MICE; MODELS, MOLECULAR; PEPTIDE FRAGMENTS; PROTEIN STRUCTURE, TERTIARY; SEQUENCE DELETION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 4444271581     PISSN: 10069305     EISSN: None     Source Type: Journal    
DOI: 10.1360/03yc0053     Document Type: Article

References (11)

1. Ming, W. J., Bersani, L., Mantovani, A., Tumor necrosis factor is chemotactic for monocytes and polymorphonuclear leukocytes, J. Immunol., 1987, 138(5): 1469-1474.
2. Kollias, G., Douni, E., Kassiotis, G. et al., On the role of tumor necrosis factor and receptors in models of multiorgan failure, rheumatoid arthritis, multiple sclerosis and inflammatory bowel disease, Immunol. Rev., 1999, 169: 175-194.
3. Maini, R. N., Elliott, M. J., Brennan, F. M. et al., Beneficial effects of tumour necrosis factor-α blockade in rheumatoid arthritis[J], Clin. Exp. Immunol., 1995, 101(2): 207-212.
4. Sandborn, W. J., Hanauer, S. B., Antitumor necrosis factor therapy for inflammatory bowel disease: A review of agents, pharmacology, clinical results, and safety, Inflamm. Bowel Dis., 1999, 5(2): 119-133.
5. Vogel, M., Miescher, S., Kuhn, S. et al., Mimicry of human IgE epitopes by anti-idiotypic antibodies, J. Mol. Biol., 2000, 298(5): 729-735.
6. MacCallum, R. M., Martin, A. C., Thornton, J. M., CH1 domains has been revised to be consistent with the three-dimensional alignments, J. Mol. Biol., 1996, 262(5): 732-745.
7. Wang Zhuozhi, Wang Yan, Li Zhengfu et al., Cloning of Fab gene of an anti-TNF-α monoclonal antibody and its expression from E. coli, Chin. J. Microbiol. Immunol. (in Chinese), 1993, 18(2): 149-151.
8. Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual (translated by Jin Dongyan, Li Mengfeng), 2nd ed., Beijing: Science Press, 1992, 888-897.
9. Zhang Wei, Shen Beifen, Li Yan et al., Applying a new PCR method to quickly prepare human TNF-α deletion mutants, Chinese Journal of Biochemistry and Molecular Biology (in Chinese), 2002, 18(5): 600-604.
10. Wang Huaichun, Essentials of Sequence Analysis (in Chinese), Beijing: Demos Surgeon Press, 1994, 38.
11. Zhang Wei, Shen Beifen, Human tumor necrosis factor-α mutant proteins and analysis of its active sites, Foreign Medical Sciences, Immunology Fascicule (in Chinese), 2002, 25(2): 61-64.