Antimicrobial, dehydroascorbate reductase, and monodehydroascorbate reductase activities of defensin from sweet potato [Ipomoea batatas (L.) Lam. 'Tainong 57'] storage roots

Journal of Agricultural and Food Chemistry

Volumn 56, Issue 9, 2008, Pages 2989-2995

  Huang, Guan Jhong ^a   Lai, Hsin Chih ^c   Chang, Yuan Shiun ^a   Sheu, Ming Jyh ^b   Lu, Te Ling ^a   Huang, Shyh Shyun ^a   Lin, Yaw Huei ^d  

^a CHINA MEDICAL UNIVERSITY   (Taiwan)

^b CHINA MEDICAL UNIVERSITY   (Taiwan)

^c CHANG GUNG UNIVERSITY   (Taiwan)

^d INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

Author keywords

Antimicrobial activity; Defensin; Dehydroascorbate reductase activity; Gene expression; Monodehydroascorbate reductase activity; Sweet potato

Indexed keywords

ANTIINFECTIVE AGENT; COMPLEMENTARY DNA; DEFENSIN; GLUTATHIONE DEHYDROGENASE (ASCORBATE); MONODEHYDROASCORBATE REDUCTASE; OXIDOREDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PLANT ROOT; SWEET POTATO;

AMINO ACID SEQUENCE; ANTI-INFECTIVE AGENTS; BASE SEQUENCE; DEFENSINS; DNA, COMPLEMENTARY; HYDROGEN-ION CONCENTRATION; IPOMOEA BATATAS; MOLECULAR SEQUENCE DATA; NADH, NADPH OXIDOREDUCTASES; OXIDOREDUCTASES; PLANT ROOTS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FUNGI; IPOMOEA BATATAS;

EID: 44349167842     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf072994j     Document Type: Article

References (31)

1. Broekaert, W. F.; Terras, F. R. G.; Cammue, B. P. A.; Osborn, R. W. Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol. 1995, 108, 1353-1358.
2. Garcia-Olmedo, F.; Molina, A.; Alamillo, J. M.; Rodriguez-Palenzuela, P. Plant defense peptides. Biopolymers 1998, 47, 479-491.
3. Thomma, B. P. H. J.; Cammue, B. P. A.; Thevissen, K. Plant defensins. Planta 2002, 216, 193-202.
4. Bloch, C., Jr.; Richardson, M. A new family of small (5 kDa) protein inhibitors of insect R-amylases from seeds of sorghum (Sorghum bicolor (L) Moench) have sequence homologies with wheat δ-purothionins. FEBS Lett. 1991, 279, 101-104.
5. Wijaya, R.; Neumann, G. M.; Condron, R.; Hughes, A. B.; Polya, G. M. Defense proteins from seed of Cassia fistula include a lipid transfer protein homologue and a protease inhibitory plant defensin. Plant Sci. 2000, 159, 243-255.
6. Colilla, F. J.; Rocher, A.; Mendez, E. α-Purothionins: amino acid sequence of two polypeptides of new family of thionins from wheat endosperm. FEBS Lett. 1990, 270, 191-194.
7. Mendez, E.; Moreno, A.; Colilla, F. J.; Pelaez, F.; Limas, G. G.; Mendez, R. Primary structure and inhibition of protein synthesis in eukaryotic cell-free system of a novel thionin, α-hordothionin, from barley endosperm. Eur. J. Biochem. 1990, 194, 533-539.
8. Osborn, R. W.; De Samblanx, G. W.; Thevissen, K.; Goderis, I.; Torrekens, S.; Van Leuven, F. V. Isolation and characterization of plant defensins from seeds of Asteraceae, Hippocastanaceae and Saxifragaceae. FEBS Lett. 1995, 368, 257-262.
9. Chen, K. C.; Lin, C. Y.; Kuan, C. C.; Sung, H. Y.; Chen, C. S. A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid. J. Agric. Food Chem. 2002, 50, 7258-7263.
10. Moreno, M.; Segura, A.; Garcia-Olmedo, F. Pseudothionin-Stl, a potato peptide active against potato pathogens. Eur. J. Biochem. 1994, 223, 135-139.
11. Kushmerick, C.; Castro, M. D.; Cruz, J. S.; Bloch, C.; Beirao, P. S. L. Functional and structural features of δ-zeathionins, a new class of sodium channel blockers. FEBS Lett. 1998, 440, 302-306.
12. Caldwell, J. E.; Abildgaard, F.; Dzakula, E.; Ming, D.; Hellekant, G.; Markley, J. L. Solution structure of the thermostable sweet-tasting protein brazzin. Nat. Struct. Biol. 1998, 5, 427-431.
13. Terras, F. R.; Goderis, I. J.; Van Leuven, F.; Vanderleyden, J.; Cammue, B. P.; Broekaert, W. F. In vitro antifungal activity of a radish (Raphanus sativus L.) seed protein homologous to non-specific lipid transfer proteins. Plant Physiol. 1992, 100, 1055-1058.
14. Terras, F. R.; Torrekens, S.; Van Leuven, F.; Osborn, R. W.; Vanderleyden, J.; Cammue, B. P.; Broekaert, W. F. A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species. FEBS Lett. 1993, 316, 233-240.
15. Epple, P.; Apel, K. Bohlmann, H. ESTs reveal a multigene family for plant defensins in Arabidopsis thaliana. FEBS Lett. 1997, 400, 168-172.
16. Harrison, P. F.; Lederberg, J. Antimicrobial Resistance; National Academies Press: Washington, DC, 1998.
17. Kragh, K. M.; Nielsen, J. E.; Nielsen, K. K.; Dreboldt, S.; Mikkelsen, J. D. Characterization and localization of new anti-fungal cysteine-rich proteins from Beta vulgaris. Mol. Plant-Microbe Interact. 1995, 8, 424-434.
18. Karunanandaa, B.; Singh, A.; Kao, T. H. Characterization of a predominantly pistil-expressed gene encoding a γ-thionin-like protein of Petunia inflata. Plant Mol. Biol. 1994, 26, 459-464.
19. Olli, S.; Kirti, P. B. Cloning, characterization and antifungal activity of defensin Tfgd1 from Trigonella foenum-graecum L. J. Biochem. Mol. Biol. 2006, 39, 278-83.
20. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989.
21. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-885.
22. Lin, Y. H.; Chen, H. L. Level and heat stability of trypsin inhibitor activity among sweet potato (Ipomoea batatas Lam.) varieties. Bot. Bull. Acad. Sin. 1980, 21, 1-13.
23. Terras, F. R. G.; Schoofs, H. M. E.; De Bolle, M. F. C.; Leuven, F. V.; Reeds, S. B.; Vanderleyden, J.; Cammue, B. P. A.; Broekaert, W. F. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J. Biol. Chem. 1992, 267, 15301-15309.
24. Broekaert, W. F.; Terras, F. R. G.; Cammue, B. P. A.; Vanderlyden, J. An automated quantitative assay for fungal growth inhibition. FEMS Microbiol. Lett. 1990, 9, 55-60.
25. Huang, D. J.; Chen, H. J.; Hou, W. C.; Lin, Y. H. Expression and localization of protein disulfide isomerase cDNA from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots. Plant Sci. 2005, 169, 776-784.
26. Yamazaki, I.; Pette, L. H. Mechanism of free radical formation and disappearance during the ascorbic acid oxidase and peroxidase reactions. Biochim. Biophys. Acta 1961, 50, 62-69.
27. Von Heijne, G. Patterns of amino acids near signal-sequence cleavage sites. Eur. J. Biochem. 1983, 133, 17-21.
28. Nielsen, H.; Engelbrecht, J.; Brunak, S.; von Heijne, G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 1997, 10, 1-6.
29. Huang, D. J.; Chen, H. J.; Hou, W. C.; Chen, T. E.; Lin, Y. H. In vitro reduction of trypsin inhibitor by purified NADPH/thioredoxin system from sprouts of sweet potato (Ipomoea batatas (L.) Lam.) storage roots. Plant Sci. 2004, 166, 435-441.
30. Chen, Z.; Gallie, D. R. Dehydroascorbate reductase affects leaf growth, development, and function. Plant Physiol. 2006, 42 (2), 775-787.
31. Trümper, S.; Follmann, H.; Häberlein, I. A novel dehydroascorbate reductase from spinach chloroplasts homologous to plant trypsin inhibitor. FEBS Lett. 1994, 352 (2), 159-162.