Culture of porcine embryonic germ cells in serum-supplemented and serum-free conditions: The effects of serum and growth factors on primary and long-term culture

Cloning and Stem Cells

Volumn 10, Issue 2, 2008, Pages 263-275

  Petkov, Stoyan G ^a   Anderson, Gary B ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BASIC FIBROBLAST GROWTH FACTOR; LEUKEMIA INHIBITORY FACTOR; STEM CELL FACTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL SURVIVAL; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYO; EMBRYO CELL; GERM CELL; KARYOTYPE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CULTURE MEDIA, SERUM-FREE; EMBRYONIC STEM CELLS; FIBROBLAST GROWTH FACTOR 2; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; KARYOTYPING; LEUKEMIA INHIBITORY FACTOR; PLURIPOTENT STEM CELLS; SERUM; STEM CELL FACTOR; SWINE;

BOVINAE; SUIDAE; SUS;

EID: 44349112926     PISSN: 15362302     EISSN: None     Source Type: Journal    
DOI: 10.1089/clo.2007.0085     Document Type: Article

References (22)

1. Amit, M., Shariki, C., Margulets, V., et al. (2004). Feeder layer and serum-free culture of human embryonic stem cells. Biol. Reprod. 70, 837-845.
2. Cheng, J., Dutra, A., Takesono, A., et al. (2004). Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media. Genesis 39, 100-104.
3. De Felici, M. (2000). Regulation of primordial germ cell development in the mouse. Int. J. Dev. Biol. 44, 575-580.
4. Dolci, S., Williams, D.E., Ernst, M.K., et al. (1991). Requirements for mast cell growth factor for primordial germ cell survival in culture. Nature 352, 809-811.
5. Durcova-Hills, G., Prelle, K., Müller, S., et al. (1998). Primary culture of porcine PGC requires LIF and porcine membrane-bound stem cell factor. Zygote 6, 271-275.
6. th ed.). (John Wiley & Sons, Inc., Hoboken, NJ) pp. 99-103.
7. Hong-mei, P., and Gui-an, C. (2006). Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines. Hum. Reprod. 21, 217-222.
8. Horii, T., Nagao, Y., Tokunaga, T., et al. (2003). Serumfree culture of murine primordial germ cells and embryonic germ cells. Theriogenology 59, 1257-1264.
9. Kühholzer, B., Baguisi, A., and Overström, E.W. (1999). Long-term culture and characterization of goat primordial germ cells. Theriogenology 53, 1071-1079
10. Labosky, P.A., Barlow, D.P., and Hogan, B.L.M. (1994). Mouse embryonic germ (EG) cell lines: transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines. Development 120, 3197-3204.
11. Lee, C.-K., and Piedrahita, J.A. (2000). Effects of growth factors and feeder cells on porcine primordial germ cells in vitro. Cloning 2, 197-205.
12. Lee, C.-K., Weaks, R.L., Johnson, G.A., et al. (2000). Effects of protease inhibitors and antioxidants on in vitro survival of porcine primordial germ cells. Biol. Reprod. 63, 887-897.
13. Li, Y., Powell, S., Brunette, E., et al. (2005). Expansion of human embryonc stem cells in defined serum-free medium devoid of animal-derived products. Biotechnol. Bioeng. 91, 688-698.
14. Matsui, Y., Zsebo, K., and Hogan, B.L.M. (1992). Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture. Cell 70, 841-847.
15. Molyneaux, K., Stallock, J., Schaible, K., et al. (2001). Time-lapse analysis of living mouse germ cell migration. Dev. Biol. 240, 488-498.
16. Mueller, S., Prelle, K., Rieger, N., et al. (1999). Chimeric pigs following blastocyst injection of transgenic porcine primordial germ cells. Mol. Reprod. Dev. 54, 244-254.
17. Park, T.S., and Han, J.Y. (2000). Derivation and characterization of pluripotent embryonic germ cells in chicken. Mol. Reprod. Dev. 56, 475-482.
18. Pesce, M., Farrace, M.G., Piacentini, M., et al. (1993). Stem cell factor and leukemia inhibitory factor promote primordial germ cell survival by suppressing programmed cell death (apoptosis). Development 118, 1089-1094.
19. Resnick, J.L., Bixler, L.S., Cheng, L., et al. (1992). Longterm proliferation of mouse primordial germ cells in culture. Nature 359, 550-551.
20. Shamblott, M.J., Axelman, J., Wang, S., et al. (1998). Derivation of pluripotent stem cells from cultured human primordial germ cells. Proc. Natl. Acad. Sci. USA 95, 13726-13731.
21. Shim, H., Gutiérres-Adán, A., Chen, L.-R., et al. (1997). Isolation of pluripotent stem cells from cultured porcine primordial germ cells. Biol. Reprod. 57, 1089-1095.
22. Takagi, Y., Talbot, N., Rexroad, C., et al. (1997). Identification of pig primordial germ cells by immunocytochemistry and lectin binding. Mol. Reprod. Dev. 46, 567-580.