Immuno-EM using colloidal metal nanoparticles and electron spectroscopic imaging for co-localization at high spatial resolution

Journal of Microscopy

Volumn 230, Issue 3, 2008, Pages 388-395

  Bleher, R ^a   Kandela, I ^a   Meyer, D A ^a   Albrecht, R M ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

Colloidal nanoparticles; Core shell nanoparticles; Electron spectroscopic imaging; EM immunolabelling; Energy filtering transmission electron microscopy; Gold; Multiple labelling; Palladium; Platinum

Indexed keywords

CORE SHELL NANOPARTICLES; ELECTRONS; GOLD NANOPARTICLES; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; IMAGE RESOLUTION; METAL NANOPARTICLES; PALLADIUM; PROTEINS; SHELLS (STRUCTURES); TISSUE;

COLLOIDAL NANOPARTICLES; CORE-SHELL NANOPARTICLES; ELECTRON SPECTROSCOPIC IMAGING; EM IMMUNOLABELING; ENERGY-FILTERING TRANSMISSION ELECTRON MICROSCOPIES; GOLD; HIGH SPATIAL RESOLUTION; IMMUNOLABELLING; LABELINGS; MULTIPLE LABELING;

PLATINUM;

ACTIN; ALPHA ACTININ; GOLD; METAL NANOPARTICLE; MYOSIN; PALLADIUM; PLATINUM;

ANTIBODY LABELING; CHEMICAL COMPOSITION; COLLOID; CONFERENCE PAPER; ENERGY FILTERED TRANSMISSION ELECTRON MICROSCOPY; PRIORITY JOURNAL; SKELETAL MUSCLE; SPECTROSCOPY;

EID: 44349096489     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2008.01997.x     Document Type: Conference Paper

References (34)

1. Adamson-Sharpe, K.M. & Ottensmeyer, F.P. (1981) Spatial resolution and detection sensitivity in microanalysis by electron energy loss selected imaging. J. Microsc. 122, 309 314.
2. Albrecht, R.M. & Meyer, D.A. (2000) The Potential for correlative and multiple-labeling with colloidal particles of differing shapes. Microsc. Microanal. 6 (Suppl 2 318 319.
3. Albrecht, R.M. & Meyer, D.A 2008) Molecular labeling for correlative microscopy: LM, LVSEM, TEM, EF-TEM and HVEM. Biological Low Voltage Scanning Electron Microscopy, Ch. 6 (ed. by H. Schatten & J. B. Pawley pp. 171 196. Springer Science, New York.
4. Albrecht, R.M., Simmons, S.R. & Pawley, J.B. (1993) Correlative video-enhanced light microscopy, high voltage transmission electron microscopy, and field emission scanning electron microscopy for the localization of colloidal gold labels. Immunocytochemistry, A practical approach, Chapter 8 (ed. by J.E. Beesley pp. 151 176. Oxford University Press, Oxford, U.K.
5. Bendayan, M. (1989) Protein A-gold and protein G-gold postembedding immunoelectron microscopy. Colloidal Gold: Principles, Methods and Applications, Vol. 1 (ed. by M.A. Hayat pp. 33 94. Academic Press, San Diego.
6. Bendayan, M., Barth, R.F., Gingras, D. et al 1989) Electron spectroscopic imaging for high-resolution immunocytochemistry: use of boronated protein A. J. Histochem. Cytochem. 37 (5 573 580.
7. Bleher, R., Meyer, D.A. & Albrecht, R.M. (2005) High resolution multiple labeling for immuno-EM applying metal colloids and energy filtering transmission electron microscopy (EFTEM). Microsc. Microanal. 11 (Suppl 2 1100 1101.
8. Egerton, R.F. (1996) Electron Energy Loss Spectroscopy in the Electron Microscope. Plenum Press, New York.
9. Faulk, W.P. & Taylor, G.M. (1971) An immunocolloid method for the electron microscope. Immunochemistry 8 (11 1081 1083
10. Frens, G. (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nat. Phys. Sci. 241 (105 20 22.
11. Horisberger, M. (1981) Colloidal gold: a cytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscopy. Scan. Electron Microsc. II, 9 31.
12. Horisberger, M. (1989) Colloidal gold for scanning electron microscopy. Colloidal Gold: Principles, Methods and Applications, Vol. 1 (ed. by M.A. Hayat pp. 217 227. Academic Press, San Diego.
13. Horisberger, M. & Rosset, J. (1977) Colloidal gold, a useful marker for transmission and scanning electron microscopy. J. Histochem. Cytochem. 25 (4 295 305.
14. Kandela, I.K., Bleher, R. & Albrecht, R.M. (2005) Correlative immunolabeling on etched Epon samples. Microsc. Microanal. 11 (Suppl 2 1098 1099.
15. Kandela, I.K. & Albrecht, R.M. (2007) Fluorescence quenching by colloidal heavy metal nanoparticles: implications for correlative fluorescence and electron microscopy studies. Scanning 29, 152 161.
16. Kandela, I.K., Bleher, R. & Albrecht, R.M. (2007) Multiple correlative immunolabeling in light and electron microscopy using fluorophores and colloidal metal particles. J. Histochem. Cytochem. 55, 983 990.
17. Kessels, M.M., Qualmann, B. & Sierralta, W.D. (1996) Immunocytochemistry by electron spectroscopic imaging using well defined boronated monovalent antibody fragments. Scan. Microsc. Suppl. 10 (Science of Biological Specimen Preparation for Microscopy 327 344.
18. Koeck, P.J.B. & Leonard, K.R. (1996) Improved immuno double labelling for cell and structural biology. Micron 27 (2 57 165.
19. Koeck, P.J.B., Schroder, R.R., Haider, M. & Leonard, K.R. (1996) Unconventional immuno double labeling by energy filtered transmission electron microscopy. Ultramicroscopy 62, 65 78.
20. Meyer, D.A. & Albrecht, R.M. (2000) Identification of multiple colloidal labels of various metallic composition by means of electron energy loss spectroscopy. Microsc. Microanal. 6 (Suppl 2 322 323.
21. Meyer, D.A. & Albrecht, R.M. (2001) The feasibility of high resolution, multiple labeling using colloidal particles of similar size but different shapes and elemental compositions. Microsc. Microanal. 7 (Suppl 2 1032 1033.
22. Meyer, D.A. & Albrecht, R.M. (2002) Size selective synthesis of colloidal platinum nanoparticles for use as high resolution EM labels. Microsc. Microanal. 8 (Suppl 2 124 125.
23. Meyer, D.A. & Albrecht, R.M. (2003) Sodium ascorbate method for the synthesis of colloidal palladium particles of different sizes. Microsc. Microanal. 9 (Suppl 2 1190 1191.
24. Meyer, D.A., Bleher, R., Kandela, I.K., Oliver, J.A. & Albrecht, R.M. (2006) The development of alternative markers for transmission electron microscopy and correlative transmission electron and light microscopies. Microsc. Microanal. 12 (Suppl 2 32 33.
25. Meyer, D.A., Oliver, J. A. & Albrecht, R.M. (2005) A method for the quadruple labeling of platelet surface epitopes for transmission electron microscopy. Microsc. Microanal. 11 (Suppl 2 142 143.
26. Nisman, R., Dellaire, G., Ren, Y., Ren, L. & Bazett-Jones, D. (2004) Application of quantum dots as probes for correlative fluorescence conventional, and energy-filtered transmission electron microscopy. J. Histochem. Cytochem. 52 (1 13 18.
27. Ottensmeyer, F.P. (1984) Electron spectroscopic imaging: parallel energy filtering and microanalysis in the fixed-beam electron microscope. J. Ultrastruct. Res. 88, 121 134.
28. Ottensmeyer, F.P. (1986) Elemental mapping by energy filtration: advantages, limitations, and compromises. Ann. N. Y. Acad. Sci. 483, 339 350.
29. Ottensmeyer, F.P. & Andrew, J.W. (1980) High resolution microanalysis of biological specimens by electron energy loss spectroscopy and by electron spectroscopic imaging. J. Ultrastruct. Res. 72, 336 348.
30. Park, K., Park, H. & Albrecht, R.M. (1989) Factors affecting the staining with colloidal gold. Colloidal Gold: Principles, Methods and Applications, Vol. 1 (ed. by M.A. Hayat pp. 489 518. Academic Press, San Diego.
31. Qualmann, B., Kessels, M.M., Klobasa, F., Jungblut, P.W. & Sierralta, W.D. (1996) Electron spectroscopic imaging of antigens by reaction with boronated antibodies. J. Microsc. 183 (1 69 77.
32. Simmons, S.R. & Albrecht, R.M. (1989) Probe size and bound label conformation in colloidal gold-ligand labels and gold-immunolabels. The Science of Biological Specimen Preparation, Scanning Microscopy. Supplement 3. (ed. by R.M. Albrecht and R.L. Ornberg pp. 27 34. Scanning Microscopy International, Chicago.
33. Tokuyasu, K.T. (1973) A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol. 57, 551 565.
34. Zsigmondy, R.A. (1906) Uber amikroskopische Goldkeime, I. Zeitschrift für Physikalische Chemie 56, 65 76.