Isolation of the exoelectrogenic bacterium Ochrobactrum anthropi YZ-1 by using a U-tube microbial fuel cell

Applied and Environmental Microbiology

Volumn 74, Issue 10, 2008, Pages 3130-3137

  Zuo, Yi ^a   Xing, Defeng ^a   Regan, John M ^a   Logan, Bruce E ^a  

^a The Pennsylvania State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY; GLUCOSE; IRON OXIDES; MICROBIAL FUEL CELLS; PATHOGENS;

BACTERIUM OCHROBACTRUM; EXOELECTROGENIC BACTERIA;

BACTERIA;

ACETIC ACID; ALCOHOL; BUTYRIC ACID; CELLOBIOSE; GLUCOSE; GLYCEROL; IRON OXIDE; LACTIC ACID; PROPIONIC ACID; RIBOSOME RNA; SUCROSE; ACETIC ACID DERIVATIVE; BACTERIAL DNA; BACTERIAL RNA; FERRIC ION; FERRIC OXIDE; RIBOSOME DNA; RNA 16S;

BACTERIUM; BIOTECHNOLOGY; ELECTRON; EXTRACTION METHOD; FUEL CELL; METAL; NUCLEIC ACID ANALYSIS; OXIDE; RNA;

ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BIOCHEMISTRY; DILUTION; ELECTRON TRANSPORT; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; OCHROBACTRUM ANTHROPI; BACTERIUM IDENTIFICATION; BIOENERGY; CARBOHYDRATE METABOLISM; CHEMISTRY; DNA DENATURATION; DNA SEQUENCE; ELECTRICITY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PSEUDOMONAS AERUGINOSA; RNA GENE; SCANNING ELECTRON MICROSCOPY; SEQUENCE HOMOLOGY; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

BACTERIA (MICROORGANISMS); OCHROBACTRUM ANTHROPI; PSEUDOMONAS AERUGINOSA;

ACETATES; BACTERIAL TYPING TECHNIQUES; BIOELECTRIC ENERGY SOURCES; CARBOHYDRATE METABOLISM; DNA, BACTERIAL; DNA, RIBOSOMAL; ELECTRICITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERRIC COMPOUNDS; GENES, RRNA; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; MOLECULAR SEQUENCE DATA; NUCLEIC ACID DENATURATION; OCHROBACTRUM ANTHROPI; PHYLOGENY; PSEUDOMONAS AERUGINOSA; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 44249125986     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02732-07     Document Type: Article

References (46)

1. Berg, G., L. Eberl, and A. Hartmann. 2005. The rhizosphere as a reservoir for opportunistic human pathogenic bacteria. Environ. Microbiol. 7:1673-1685.
2. Bond, D. R., D. E. Holmes, L. M. Tender, and D. R. Lovley. 2002. Electrode-reducing microorganisms that harvest energy from marine sediments. Science 295:483-485.
3. Bond, D. R., and D. R. Lovley. 2003. Electricity production by Geobacter sulfurreducens attached to electrodes. Appl. Environ. Microbiol. 69:1548-1555.
4. Bond, D. R., and D. R. Lovley. 2005. Evidence for involvement of an electron shuttle in electricity generation by Geothrix fermentons. Appl. Environ. Microbiol. 71:2186-2189.
5. Bretschger, O., A. Obraztsova, C. A. Sturm, I. S. Chang, Y. A. Gorby, S. B. Reed, D. E. Culley, C. L. Reardon, S. Barua, M. F. Romine, J. Zhou, A. S. Beliaev, R. Bouhenni, D. Saffarini, F. Mansfeld, B. Kim, J. K. Fredrickson, and K. H. Nealson. 2007. Current production and metal oxide reduction by Shewanella oneidensis MR-1 wild type and mutants. Appl. Environ. Microbiol. 73:7003-7012.
6. Caccavo, F., D. J. Lonergan, D. R. Lovley, M. Davis, J. F. Stolz, and M. J. McInerney. 1994. Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism. Appl. Environ. Microbiol. 60:3752-3759.
7. Carlson, C. A., and J. L. Ingraham. 1983. Comparison of denitrification by Pseudomonas stutzeri, Pseudomonas aeruginosa, and Paracoccus denitrificans. Appl. Environ. Microbiol. 45:1247-1253.
8. Chaudhuri, S. K., and D. R. Lovley. 2003. Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells. Nat. Biotechnol. 21:1229-1232.
9. Cheng, S., and B. E. Logan. 2007. Ammonia treatment of carbon cloth anodes to enhance power generation of microbial fuel cells. Electrochem. Commun. 9:492-496.
10. Finneran, K. T., C. V. Johnsen, and D. R. Lovley. 2003. Rhodoferax ferrireducens sp. nov., a psychrotolerant, facultatively anaerobic bacterium that oxidizes acetate with the reduction of Fe(III). Int. J. Syst. Evol. Microbiol. 53:669-673.
11. Fredrickson, J. K., S. Kota, R. K. Kukkadapu, C. Liu, and J. M. Zachara. 2003. Influence of electron donor/acceptor concentrations on hydrous ferric oxide (HFO) bioreduction. Biodegradation 14:91-103.
12. Gorby, Y. A., Y. S. Yanina, J. S. McLean, K. M. Rosso, D. Moyles, A. Dohnalkova, T. J. Beveridge, I. S. Chang, B. H. Kim, K. S. Kim, D. E. Culley, S. B. Reed, M. F. Romine, D. A. Saffarini, E. A. Hill, L. Shi, D. A. Elias, D. W. Kennedy, G. Pinchuk, K. Watanabe, S. Ishii, B. Logan, K. H. Nealson, and J. K. Fredrickson. 2006. Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms. Proc. Natl. Acad. Sci. USA 103:11358-11363.
13. Holmes, B., M. Popoff, M. Kiredjian, and K. Kersters. 1988. Ochrobactrum anthropi gen. nov., sp. nov. from human clinical specimens and previously known as group Vd. Int. J. Syst. Bacteriol. 38:406-416.
14. Holmes, D. E., D. R. Bond, and D. R. Lovley. 2004. Electron transfer by Desulfobulbus propionicus to Fe(III) and graphite electrodes. Appl. Environ. Microbiol. 70:1234-1237.
15. Holmes, D. E., J. S. Nicoll, D. R. Bond, and D. R. Lovley. 2004. Potential role of a novel psychrotolerant member of the family Geobacteraceae, Geopsychrobacter electrodiphilus gen. nov., sp. nov., in electricity production by a marine sediment fuel cell. Appl. Environ. Microbiol. 70:6023-6030.
16. Kesseru, P., I. Kiss, Z. Bihari, and B. Polyák. 2002. The effects of NaCl and some heavy metals on the denitrification activity of Ochrobactrum anthropi. J. Basic Microbiol. 42:268-276.
17. Kim, B. H., H. J. Kim, M. S. Hyun, and D. S. Park. 1999. Direct electrode reaction of Fe(III) reducing bacterium, Shewanella putrefaciens. J. Microbiol. Biotechnol. 9:127-131.
18. Kim, H. J., H. S. Park, M. S. Hyun, I. S. Chang, M. Kim, and B. H. Kim. 2002. A mediator-less microbial fuel cell using a metal reducing bacterium, Shewanella putrefaciens. Enzyme Microb. Technol. 30:145-152.
19. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111-120.
20. Kumar, S., K. Tamura, and M. Nei. 2004. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform. 2:150-163.
21. Lee, J., N. T. Phung, I. S. Chang, B. H. Kim, and H. C. Sung. 2003. Use of acetate for enrichment of electrochemically active microorganisms and their 16S rDNA analyses. FEMS Microbiol. Lett. 223:185-191.
22. Liu, H., and B. E. Logan. 2004. Electricity generation using an air-cathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane. Environ. Sci. Technol. 38:4040-4046.
23. Logan, B. E. 2008. Microbial fuel cells. John Wiley & Sons, New York, NY.
24. Logan, B. E., S. Cheng, V. Watson, and G. Estadt. 2007. Graphite fiber brush anodes for increased power production in air-cathode microbial fuel cells. Environ. Sci. Technol. 41:3341-3346.
25. Logan, B. E., and J. M. Regan. 2006. Electricity-producing bacterial communities in microbial fuel cells. Trends Microbiol. 14:12-518.
26. Lovley, D. R. 2006. Bug juice: harvesting electricity with microorganisms. Nat. Rev. Microbiol. 4:497-508.
27. Lovley, D. R., D. E. Holmes, and K. P. Nevin. 2004. Dissimilatory Fe(III) and Mn(IV) reduction. Adv. Microb. Physiol. 49:219-286.
28. Lovley, D. R., and E. J. P. Philips. 1986. Organic matter mineralization with reduction of ferric iron in anaerobic sediments. Appl. Environ. Microbiol. 51:683-689.
29. Milliken, C. E., and H. D. May. 2007. Sustained generation of electricity by the spore-forming, Gram-positive, Desulfitobacterium hafniense strain DCB2. Appl. Microbiol. Biotechnol. 73:1180-1189.
30. Min, B., S. Cheng, and B. E. Logan. 2005. Electricity generation using membrane and salt bridge microbial fuel cells. Water Res. 39:1675-1686.
31. Myers, C. R., and J. M. Myers. 1992. Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1. J. Bacteriol. 174:3429-3438.
32. Park, D. H., and J. G. Zeikus. 2002. Impact of electrode composition on electricity generation in a single compartment fuel ceil using Shewanella putrefaciens. Appl. Microbiol. Biotechnol. 59:58-61.
33. Park, D. H., and J. G. Zeikus. 2003. Improved fuel cell and electrode designs for producing electricity from microbial degradation. Biotechnol. Bioeng. 81:348-355.
34. Park, H. S., B. H. Kim, H. S. Kim, H. J. Kim, G. T. Kim, M. Kim, I. S. Chang, Y. K. Park, and H. I. Chang. 2001. A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell. Anaerobe 7:297-306.
35. Pham, C. A., S. J. Jung, N. T. Phung, J. Lee, I. S. Chang, B. H. Kim, H. Yi, and J. Chun. 2003. A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Aeromonas hydrophila, isolated from a microbial fuel cell. FEMS Microbiol. Lett. 223:129-134.
36. Rabaey, K., N. Boon, S. D. Siciliano, M. Verhaege, and W. Verstraete. 2004. Biofuel cells select for microbial consortia that self-mediate electron transfer. Appl. Environ. Microbiol. 70:5373-5382.
37. Reguera, G., K. D. McCarthy, T. Mehta, J. S. Nicoll, M. T. Tuominen, and D. R. Lovley. 2005. Extracellular electron transfer via microbial nanowires. Nature 435:1098-1101.
38. Ren, N., D. Xing, B. E. Rittmann, L. Zhao, T. Xie, and X. Zhao. 2007. Microbial community structure of ethanol type fermentation in bio-hydrogen production. Environ. Microbiol. 9:1112-1125.
39. Richter, H., M. Lanthier, K. P. Nevin, and D. R. Lovley. 2007. Lack of electricity production by Pelobacter carbinolicus indicates that the capacity for Fe(III) oxide reduction does not necessarily confer electron transfer ability to fuel cell anodes. Appl. Environ. Microbiol. 73:5347-5353.
40. Scott, J. H., and K. H. Nealson. 1994. A biochemical study of the intermediary carbon metabolism of Shewanella putrefaciens. J. Bacteriol. 176:3408-3411.
41. Trujillo, M. E., A. Willems, A. Abril, A. Planchuelo, R. Rivas, D. Ludeña, P. F. Mateos, E. Martínez-Molina, and E. Velázquez. 2005. Nodulation of Lupinus albus by strains of Ochrobactrum lupini sp. nov. Appl. Environ. Microbiol. 71:1318-1327.
42. Watanabe, K., Y. Kodama, and S. Harayama. 2001. Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting. J. Microbiol. Methods 44:253-262.
43. Xing, D., N. Ren, Q. Li, M. Lin, A. Wang, and L. Zhao. 2006. Ethanoligenens harbinense gen. nov., sp. nov., isolated from molasses waste water. Int. J. Syst. Evol. Microbiol. 56:755-760.
44. Xing, D., Y. Zuo, S. Cheng, J. M. Regan, and B. E. Logan. Electricity generation by Rhodopseudomonas palustris DX-1. Environ. Sci. Technol., in press.
45. Zuo, Y., P.-C. Maness, and B. E. Logan. 2006. Electricity production from steam exploded corn stover biomass. Energ. Fuel 20:1716-1721.
46. Zurdo-Piñeiro, J. L., R. Rivas, M. E. Trujillo, N. Vizcaíno, J. A. Carrasco, M. Chamber, A. Palomares, P. F. Mateos, E. Martínez-Molina, and E. Velázquez. 2007. Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain. Int. J. Syst. Evol. Microbiol. 57:784-788.