Evaluation of three polymerase chain reaction techniques for detection of Brucella DNA in peripheral human blood

Canadian Journal of Microbiology

Volumn 54, Issue 5, 2008, Pages 352-357

  Baddour, Manal M ^a,c   Alkhalifa, Dalal H ^b  

^a ALEXANDRIA UNIVERSITY   (Egypt)

^b Girls College of Education Riyadh   (Saudi Arabia)

^c KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

Brucella; Comparison; Diagnosis; Human blood; PCR

Indexed keywords

BACTERIA; BLOOD; DIAGNOSIS; DNA; GENE ENCODING;

BRUCELLOSIS; POLYMERASE CHAIN REACTION; PRIMERS;

ENZYME ACTIVITY;

BACTERIAL DNA; OUTER MEMBRANE PROTEIN; RIBOSOME RNA;

BACTERIAL DISEASE; BLOOD; COMPARATIVE STUDY; DETECTION METHOD; EXTRACTION METHOD; GENE EXPRESSION; GENOME; HOMINID; LABORATORY METHOD; POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS;

ARTICLE; BACTERIAL GENOME; BLOOD ANALYSIS; BRUCELLA; BRUCELLA ABORTUS; BRUCELLOSIS; CONTROLLED STUDY; DNA DETERMINATION; DNA EXTRACTION; GENE AMPLIFICATION; HUMAN; LABORATORY DIAGNOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA SEQUENCE; SENSITIVITY AND SPECIFICITY; ZOONOSIS;

ADOLESCENT; ADULT; AGED; BACTERIOLOGICAL TECHNIQUES; BLOOD; BRUCELLA; BRUCELLOSIS; DNA PRIMERS; DNA, BACTERIAL; FEMALE; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BRUCELLA; BRUCELLA MELITENSIS BIOVAR ABORTUS;

EID: 43949104050     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W08-017     Document Type: Article

References (22)

1. Al Dahouk, S., Tomaso, H., Nockler, K., Neubauer, H., and Frangoulidis, D. 2003. Laboratory-based diagnosis of brucellosis - a review of the literature. Part I. Techniques for direct detection and identification of Brucella spp. Clin. Lab. (Zaragoza), 49: 487-505.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. 2002. Short protocols in molecular biology. 5th ed. John Wiley and Sons, Inc., USA.
3. Baily, G.G., Kraahn, J.B., Drasar, B.S., and Stoker, N.G. 1992. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J. Trop. Med. Hyg. 95: 271-275. PMID:1495123.
4. Casanas, M.C., Queipo-Ortuno, M.I., Rodriguuez-Torres, A., Orduna, A., and Calmenero, J.D. 2001. Specificity of a polymerase chain reaction assay of a target sequence on the 31-kilodalton Brucella antigen DNA used to diagnose human brucellosis. Eur. J. Clin. Microbiol. Infect. Dis. 20: 127-131. doi:10.1007/s100960000445. PMID:11305467.
5. Kwok, S., and Higushi, R. 1989. Avoiding false positives with PCR. Nature, 339: 237-238. doi:10.1038/339237a0. PMID:2716852.
6. Leal-Klevezas, D.S., Martinez-Vazquez, I.O., and Martinez-Soriano, J.P. 1995. Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J. Clin. Microbiol. 33: 3087-3090. PMID:8586678.
7. Leyla, G., Kadri, G.C., and Umran, O. 2003. Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet. Microbiol. 93: 53-61. doi:10.1016/S0378-1135(02)00442-X. PMID:12591206.
8. Matar, G.M., Khneisser, I.A., and Abdelnoor, A.M. 1996. Rapid laboratory confirmation of human brucellosis by PCR analysis of a target sequence on the 31-kilodalton Brucella antigen DNA. J. Clin. Microbiol. 34: 477-478. PMID:8789045.
9. Miller, S.A., Dykes, D.D., and Pandolesky, H.F. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16: 1215. doi:10.1093/nar/16.3.1215. PMID:3344216.
10. Moyer, N.P., Evins, G.M., Pigott, N.E., Hudson, J.D., Farshy, C.E., Feeley, J.C., and Hausler, W.J., Jr. 1987. Comparison of serologic screening tests for brucellosis. J. Clin. Microbiol. 25: 1969-1972. PMID:3117844.
11. Navarro, E., Fernandez, J.A., Escribano, J., and Solera, J. 1999. PCR assay for diagnosis of human brucellosis. J. Clin. Microbiol. 37: 1654-1655. PMID:10328689.
12. Navarro, E., Escribano, J., Fernandez, J.A., and Solera, J. 2002. Comparison of three different PCR methods for detection of Brucella spp. in human blood samples. FEMS Immunol. Med. Microbiol. 34: 147-151. doi:10.1111/j.1574-695X.2002.tb00616.x. PMID:12381466.
13. Nimri, L.F. 2003. Diagnosis of recent and relapsed cases of human brucellosis by PCR assay. BMC Infect. Dis. 3: 5. doi:10.1186/1471-2334-3- 5. PMID:12718759.
14. Pike, R.M., Sulkin, S.E., and Schulze, M.L. 1965. Continuing importance of laboratory-acquired infections. Am. J. Public Health, 55: 190-199.
15. Queipo-Ortuno, M.I., Morata, P., Ocon, P., Manchado, P., and de Dios Colmenero, J. 1997. Rapid diagnosis of human brucellosis by peripheral-blood PCR assay. J. Clin. Microbiol. 35: 2927-2930. PMID:9350761.
16. Romero, C., Gamazo, C., Pardo, M., and Lopez-Goni, I. 1995. Specific detection of Brucella DNA by PCR. J. Clin. Microbiol. 33: 615-617. PMID:7538508.
17. Solera, J., Martinez-Alfaro, E., and Espinosa, A. 1997. Recognition and optimum treatment of brucellosis. Drugs, 53: 245-256. doi:10.2165/00003495-199753020-00005. PMID:9028744.
18. World Health Organization. 1997. Fact sheet N173, July 1997. World Health Organization, Geneva, Switzerland.
19. Yagupsky, P. 1999. Detection of brucellae in blood cultures. J. Clin. Microbiol. 37: 3437-3442. PMID:10523530.
20. Young, E. 1995. Brucella species. In Principles and practice of infectious diseases, 4th ed. Edited by G.L. Mandell, J.E. Bennett, and R. Dolin. Churchill Livingstone, New York. pp. 2053-2060.
21. Young, E.J. 1997. Especies de Brucella. In Enfermedades infecciosas. Principios y practica Edited by G.L. Mandell, J.E. Bennett, and R. Dolin. Medica Panamericana, Buenos Aires. pp. 2300-2307.
22. Zerva, L., Bourantas, K., Mitka, S., Kansouzidou, A., and Legakis, N.J. 2001. Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR. J. Clin. Microbiol. 39: 1661-1664. doi:10.1128/JCM.39.4.1661-1664.2001. PMID:11283112.