Efficient recovery of recombinant human erythropoietin from milk of transgenic pigs by two-step pretreatment

Biotechnology and Bioprocess Engineering

Volumn 13, Issue 2, 2008, Pages 189-196

  Lee, Eun Gyo ^a   Lee, Seung Hui ^a   Park, Kyoung Mi ^a   Baek, Jung Eun ^a   Park, Jin Ki ^b   Chang, Won Kyong ^b   Jung, Joon Ki ^a   Chung, Bong Hyun ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b National Institute of Animal Science   (South Korea)

Author keywords

Divalent metal; Erythropoietin; Precipitation; Recovery; Transgenic pig

Indexed keywords

AMMONIUM SULFATE; CALCIUM CHLORIDE; COPPER SULFATE; DIVALENT CATION; MILK PROTEIN; NICKEL SULFATE; RECOMBINANT ERYTHROPOIETIN; ZINC CHLORIDE;

ARTICLE; CONCENTRATION (PARAMETERS); CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MILK LEVEL; DRUG PURIFICATION; FLOCCULATION; NONHUMAN; PH MEASUREMENT; PRECIPITATION; PROTEIN AGGREGATION; SWINE; TRANSGENIC ANIMAL;

ANIMALIA; SUIDAE;

EID: 43749118721     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-007-0158-x     Document Type: Article

References (21)

1. Gordon, J. W., G. A. Scangos, D. J. Plotkin, J. A. Barbosa, and F. H. Ruddle (1980) Genetic transformation of mouse embryos by microinjection of purified DNA. Proc. Natl. Acad. Sci. USA 77: 7380-7384.
2. Choi, Y. S., D. Y. Lee, I. Y. Kim, H. J. Kim, H. W. Park, T. B. Choe, and I. H. Kim (2007) Enhancement of erythropoietin production in recombinant Chinese hamster ovary cells by sodium lactate addition. Biotechnol. Bioprocess Eng. 12: 60-72.
3. Jo, S. H., S. Y. Kwon, D. S. Park, K. S. Yang, J. W. Kim, K. T. Lee, S. S. Kwak, and H. S. Lee (2006) High-yield production of functional human lactoferrin in transgenic cell cultures of Siberian ginseng (Acanthopanax senticosus). Biotechnol. Bioprocess Eng. 11: 442-448.
4. Das, R. C. (2001) Production of therapeutic proteins from transgenic animals. Biobusiness 19: 60-64.
5. Hughes, H. C. (1986) Swine in cardiovascular research. Lab. Anim. Sci. 36: 348-350.
6. Paleyanda, R. K., W. H. Velander, T. K. Lee, D. H. Scandella, F. C. Gwazdauskas, J. W. Knight, L. W. Hoyer, W. N. Drohan, and H. Lubon (1997) Transgenic pigs produce functional human factor VIII in milk. Nat. Biotechnol. 15: 971-975.
7. Swanson, M. E., M. J. Martin, J. K. O'Donnell, K. Hoover, W. Lago, V. Huntress, C. T. Parsons, C. A. Pinkert, S. Pilder, and J. S. Logan (1992) Production of functional human hemoglobin in transgenic swine. Biotechnology 10: 557-559.
8. Velander, W. H., J. L. Johnson, R. L. Page, C. G. Russell, A. Subramanian, T. D. Wilkins, F. C. Gwazdauskas, C. Pittius, and W. N. Drohan (1992) High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C. Proc. Natl. Acad. Sci. USA 89: 12003-12007.
9. Wall, R. J., V. G. Pursel, A. Shamay, R. A. McKnight, C. W. Pittius, and L. Hennighausen (1991) High-level synthesis of a heterologous milk protein in the mammary glands of transgenic swine. Proc. Natl. Acad. Sci. USA 88: 1696-1700.
10. Weidle, U. H., H. Lenz, and G. Brem (1991) Genes encoding a mouse monoclonal antibody are expressed in transgenic mice, rabbits and pigs. Gene 98: 185-191.
11. Kim, H. W. (2007) Engineering blood cells and proteins as blood substitutes: A short review. Biotechnol. Bio-process Eng. 12: 43-47.
12. Lappin, T. R., A. P. Maxwell, and P. G. Johnston (2002) EPO's alter ego: erythropoietin has multiple actions. Stem Cells 20: 485-492.
13. Park, J. K., Y. K. Lee, P. Lee, H. J. Chung, S. Kim, H. G. Lee, M. K. Seo, J. H. Han, C. G. Park, H. T. Kim, Y. K. Kim, K. S. Min, J. H. Kim, H. T. Lee, and W. K. Chang (2006) Recombinant human erythropoietin produced in milk of transgenic pigs. J. Biotechnol. 122: 362-371.
14. Toledo, J. R., O. Sanchez, R. M. Segui, G. Garcia, M. Montanez, P. A. Zamora, M. P. Rodriguez, and J. A. Cremata (2006) High expression level of recombinant human erythropoietin in the milk of non-transgenic goats. J. Biotechnol. 123: 225-235.
15. Nikolov, Z. L. and S. L. Woodard (2004) Downstream processing of recombinant proteins from transgenic feedstock. Curr. Opin. Biotechnol. 15: 479-486.
16. Morcol, T., Q. He, and S. J. D. Bell (2001) Model process for removal of caseins from milk of transgenic animals. Biotechnol. Prog. 17: 577-582.
17. Lee, V. W. and K. P. Antonsen (2003) Purification of alpha-1 proteinase inhibitor. US Patent 6,525,176.
18. Wilkins, T. D. and W. Velander (1992) Isolation of recombinant proteins from milk. J. Cell. Biochem. 49: 333-338.
19. 2+-selective purification of recombinant proteins from the milk of transgenic animals. J. Chromatogr. A 799: 125-137.
20. Morcol, T. and S. J. D. Bell (2001) Method for processing milk. US Patent 6,183,803.
21. Bell, S. J. D., T. Morcol, and Q. He (2002) Therapeutic calcium phosphate particles and methods of manufacture and use. US Patent 6,355,271.