Expression, purification, crystallization and preliminary x-ray crystallographic analysis of CnrX from Cupriavidus metallidurans CH34

Journal of Microbiology and Biotechnology

Volumn 18, Issue 1, 2008, Pages 43-47

  Kim, Kook Han ^a   Jung, Eun Jung ^b   Im, Hana ^b   Van Der Lelie, Daniel ^c   Kim, Eunice Eunkyeong ^a  

^a Korea Institute of Science and Technology   (South Korea)

^b Sejong University   (South Korea)

^c BROOKHAVEN NATIONAL LABORATORY   (United States)

Author keywords

CnrX; Crystallization; Cupriavidus metallidurans CH34; Nickel resistance; Periplasmic sensor

Indexed keywords

BACTERIAL PROTEIN; COBALT; DITHIOTHREITOL; GLYCEROL; LITHIUM CHLORIDE; MONOMER; NICKEL; PROTEIN CNRX; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; CRYSTALLIZATION; CUPRIAVIDUS; CUPRIAVIDUS METALLIDURANS; DIFFRACTION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; HANGING DROP VAPOR DIFFUSION METHOD; MOLECULAR CLONING; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TRANSCRIPTION REGULATION; X RAY CRYSTALLOGRAPHY; AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; CHEMISTRY; CYTOPLASM; DRUG EFFECT; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; SEQUENCE ALIGNMENT; STRUCTURE ACTIVITY RELATION;

CUPRIAVIDUS METALLIDURANS; ESCHERICHIA COLI;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; COBALT; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; CUPRIAVIDUS; DRUG RESISTANCE, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; NICKEL; PERIPLASM; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 43749099051     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

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