Photodynamic Treatment of Endodontic Polymicrobial Infection In Vitro

Journal of Endodontics

Volumn 34, Issue 6, 2008, Pages 728-734

  Fimple, Jacob Lee ^a   Fontana, Carla Raquel ^b   Foschi, Federico ^b   Ruggiero, Karriann ^b   Song, Xiaoqing ^b   Pagonis, Tom C ^a   Tanner, Anne C R ^b   Kent, Ralph ^b   Doukas, Apostolos G ^c   Stashenko, Philip P ^b   Soukos, Nikolaos S ^b  

^a HARVARD SCHOOL OF DENTAL MEDICINE   (United States)

^b FORSYTH INSTITUTE   (United States)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Biofilms; endodontic polymicrobial infection; methylene blue; photodynamic therapy; root canals

Indexed keywords

BACTERIAL DNA; ENZYME INHIBITOR; METHYLENE BLUE;

ANAEROBIC BACTERIUM; ARTICLE; BACTERIAL COUNT; BACTERIAL INFECTION; BIOFILM; CONFOCAL MICROSCOPY; DIODE LASER; DRUG EFFECT; HUMAN; MICROBIOLOGY; PHOTOCHEMOTHERAPY; SCANNING ELECTRON MICROSCOPY; STATISTICAL MODEL; TOOTH PULP; TOOTH PULP DISEASE;

BACTERIA, ANAEROBIC; BACTERIAL INFECTIONS; BIOFILMS; COLONY COUNT, MICROBIAL; DENTAL PULP CAVITY; DENTAL PULP NECROSIS; DNA, BACTERIAL; ENZYME INHIBITORS; HUMANS; LASERS, SEMICONDUCTOR; LINEAR MODELS; METHYLENE BLUE; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, SCANNING; PHOTOCHEMOTHERAPY;

EID: 43549111574     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joen.2008.03.011     Document Type: Article

References (35)

1. Sjögren U., Figdor D., Persson S., and Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 30 (1997) 297-306
2. Byström A., and Sundqvist G. Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. Oral Surg Oral Med Oral Pathol 55 (1983) 307-312
3. Sundqvist G., Figdor D., Persson S., and Sjögren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol 85 (1998) 86-93
4. Siqueira J.F., Araújo M.C.P., Garcia P.F., Fraga R.C., and Dantas C.J.S. Histological evaluation of the effectiveness of five instrumentation techniques for cleaning the apical third of root canals. J Endod 23 (1997) 499-502
5. Rolph H.J., Lennon A., Riggio M.P., et al. Molecular identification of microorganisms from endodontic infections. J Clin Microbiol 39 (2001) 3282-3289
6. Siqueira Jr. J.F. Aetiology of root canal treatment failure: why well-treated teeth can fail. Int Endod J 34 (2001) 1-10
7. Hancock III H.H., Sigurdsson A., Trope M., and Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg Oral Med Oral Pathol 91 (2001) 579-586
8. Pinheiro E.T., Gomes B.P., Ferraz C.C., Sousa E.L., Teixeira F.B., and Souza-Filho F.J. Microorganisms from canals of root filled teeth with periapical lesions. Int Endod J 36 (2003) 1-11
9. Dougherty T.J., Gomer C.J., Henderson B.W., et al. Photodynamic therapy. J Natl Cancer Inst 90 (1998) 889-905
10. Wilson M. Photolysis of oral bacteria and its potential use in the treatment of caries and periodontal disease: a review. J App Bacteriol 75 (1993) 299-306
11. Soukos N.S., Ximenez-Fyvie L.A., Hamblin M.R., Socransky S.S., and Hasan T. Targeted antimicrobial photochemotherapy. Antimicrob Agents Chemother 42 (1998) 2595-2601
12. Silbert T., Bird P.S., Milburn G.J., and Walsh L. Disinfection of root canals by laser dye photosensitization. J Dent Res 79 Sp Issue (2000) 569
13. Seal G.J., Ng Y.L., Spratt D., Bhatti M., and Gulabivala K. An in vitro comparison of the bactericidal efficacy of lethal photosensitization or sodium hyphochlorite irrigation on Streptococcus intermedius biofilms in root canals. Int Endod J 35 (2002) 268-274
14. Garcez A.S., Nunez S.C., Lage-Marques J.L., Jorge A.O., and Ribeiro M.S. Efficiency of NaOCl and laser-assisted photosensitization on the reduction of Enterococcus faecalis in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 102 (2006) 93-98
15. Soukos N.S., Chen P.S., Morris J.T., et al. Photodynamic therapy for endodontic disinfection. J Endod 32 (2006) 979-984
16. Williams J.A., Pearson G.J., and Colles M.J. Antibacterial action of photoactivated disinfection {PAD} used on endodontic bacteria in planktonic suspension and in artificial and human root canals. J Dent 34 (2006) 363-371
17. Garcez A.S., Ribeiro M.S., Tegos G.P., Nunez S.C., Jorge A.O., and Hamblin M.R. Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection. Lasers Surg Med 39 (2007) 59-66
18. George S., and Kishen A. Photophysical, photochemical, and photobiological characterization of Methylene blue formulations for light-activated root canal disinfection. J Biomed Opt 12 (2007) 34029-34038
19. Foschi F., Fontana C.R., Ruggiero K., et al. Photodynamic inactivation of Enterococcus faecalis in dental root canals in vitro. Lasers Surg Med 39 (2007) 782-787
20. Bergmans L., Moisiadis P., Huybrechts B., Van Meerbeek B., Quirynen M., and Lambrechts P. Effect of photo-activated disinfection on endodontic pathogens ex vivo. Int Endod J 41 (2008) 227-239
21. Bonsor S.J., Nichol R., Reid T.M., and Pearson G.J. An alternative regimen for root canal disinfection. Br Dent J 22 (2006) 101-105
22. Bonsor S.J., Nichol R., Reid T.M., and Pearson G.J. Microbiological evaluation of photo-activated disinfection in endodontics (an in vivo study). Br Dent J 25 (2006) 337-341
23. Garcez A.S., Nunez S.C., Hamblin M.R., and Ribeiro M.S. Antimicrobial effects of photodynamic therapy on patients with necrotic pulps and periapical lesion. J Endod 34 (2008) 138-142
24. Wainwright M., Phoenix D.A., Marland J., Wareing D.R.A., and Bolton F.J. A study of photobactericidal activity in the phenothiazinium series. FEMS Immunol Med Microbiol 19 (1997) 75-80
25. Usacheva M.N., Teichert M.C., and Biel M.A. The interaction of lipopolysaccharides with phenothiazine dyes. Lasers Surg Med 33 (2003) 311-319
26. Sundqvist G. Associations between microbial species in dental root canal infections. Oral Microbiol Immunol 7 (1992) 257-262
27. Bhatti M., MacRobert A., Meghji S., Henderson B., and Wilson M. Effect of dosimetric and physiological factors on the lethal photosensitization of Porphyromonas gingivalis in vitro. Photochem Photobiol 65 (1997) 1026-1031
28. Soukos N.S., Mulholland S.E., Socransky S.S., and Doukas A.G. Photodestruction of human dental plaque bacteria: enhancement of the photodynamic effect by photomechanical waves in an oral biofilm model. Lasers Surg Med 33 (2003) 161-168
29. Socransky S.S., Haffajee A.D., Smith C., et al. Use of checkerboard DNA-DNA hybridization to study complex microbial ecosystems. Oral Microbiol Immunol 19 (2004) 352-362
30. Ramsköld L.O., Fong C.D., and Stromberg T. Thermal effects and antibacterial properties of energy levels required to sterilize stained root canals with an Nd:YAG laser. J Endod 23 (1997) 96-100
31. Harris F., Chatfield L.K., and Phoenix D.A. Phenothiazinium based photosensitisers: photodynamic agents with a multiplicity of cellular targets and clinical applications. Curr Drug Targets 6 (2005) 615-627
32. Absi E.G., Addy M., and Adams D. Dentine hypersensitivity: a study of the patency of dentinal tubules in sensitive and non-sensitive cervical dentine. J Clin Periodontol 14 (1987) 280-284
33. Berkiten M., Okar I., and Berkiten R. In vitro study of the penetration of Streptococcus sanguis and Prevotella intermedia strains into human dentinal tubules. J Endod 26 (2000) 236-239
34. Siqueira Jr. J.F., De Uzeda M., and Fonseca M.E. A scanning electron microscopic evaluation of in vitro dentinal tubules penetration by selected anaerobic bacteria. J Endod 22 (1996) 308-310
35. Henderson B.W. Probing the effects of photodynamic therapy through in vivo-in vitro methods. In: Kesse l.D. (Ed). Photodynamic therapy of neoplastic disease (1990), CRC Press, Boca Raton, FL 169-188