A simple assay to measure phagocytosis of live bacteria

Clinical Chemistry

Volumn 54, Issue 5, 2008, Pages 911-915

  Bicker, Heike ^a   Höflich, Conny ^a   Wolk, Kerstin ^a   Vogt, Katrin ^a   Volk, Hans Dieter ^a   Sabat, Robert ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; PLASMID VECTOR;

ARTICLE; BLOOD SAMPLING; CHANNEL GATING; ESCHERICHIA COLI; FLOW CYTOMETRY; GRANULOCYTE; HEPARINIZATION; HUMAN; IMMUNE DEFICIENCY; IMMUNOSUPPRESSIVE TREATMENT; KIDNEY TRANSPLANTATION; MONOCYTE; NONHUMAN; PARALYSIS; PHAGOCYTOSIS; POSTOPERATIVE PERIOD; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SEPSIS;

ESCHERICHIA COLI; FLOW CYTOMETRY; GRANULOCYTES; GREEN FLUORESCENT PROTEINS; HUMANS; IMMUNE TOLERANCE; KIDNEY TRANSPLANTATION; MONOCYTES; PHAGOCYTOSIS; POSTOPERATIVE COMPLICATIONS; SEPSIS;

BACTERIA (MICROORGANISMS);

EID: 43449136766     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2007.101337     Document Type: Article

References (17)

1. Malech HL, Hickstein DD. Genetics, biology and clinical management of myeloid cell primary immune deficiencies: chronic granulomatous disease and leukocyte adhesion deficiency. Curr Opin Hematol 2007;14:29-36.
2. Hutchinson P, Chadban SJ, Atkins RC, Holdsworth SR. Laboratory assessment of immune function in renal transplant patients. Nephrol Dial Transplant 2003;18:983-9.
3. Serlenga E, Garofalo AR, De Pergola G, Ventura MT, Tortorella C, Antonaci S. Polymorphonuclear cell-mediated phagocytosis and superoxide anion release in insulin-dependent diabetes mellitus. Cytobios 1993;74:189-95.
4. Hornef MW, Wick MJ, Rhen M, Normark S. Bacterial strategies for overcoming host innate and adaptive immune responses. Nat Immunol 2002;3:1033-40.
5. Lei B, DeLeo FR, Hoe NP, Graham MR, Mackie SM, Cole RL, et al. Evasion of human innate and acquired immunity by a bacterial homolog of CD11b that inhibits opsonophagocytosis. Nat Med 2001;7:1298-305.
6. Ojielo CI, Cooke K, Mancuso P, Standiford TJ, Olkiewicz KM, Clouthier S, et al. Defective phagocytosis and clearance of Pseudomonas aeruginosa in the lung following bone marrow transplantation. J Immunol 2003;171:4416-24.
7. Foukas LC, Katsoulas HL, Paraskevopoulou N, Metheniti A, Lambropoulou M, Marmaras VJ. Phagocytosis of Escherichia coli by insect hemocytes requires both activation of the Ras/mitogen-activated protein kinase signal transduction pathway for attachment and beta3 integrin for internalization. J Biol Chem 1998;273:14813-8.
8. Grunwald U, Fan X, Jack RS, Workalemahu G, Kallies A, Stelter F, Schutt C. Monocytes can phagocytose Gram-negative bacteria by a CD14-dependent mechanism. J Immunol 1996;157:4119-25.
9. Cuffini AM, Tullio V, Giacchino F, Bonino A, Mandras N, Bianchi N, et al. Improved phagocyte response by co-amoxiclav in renal transplant recipients. Transplantation 2001;71:575-7.
10. Cormack BP, Valdivia RH, Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996;173:33-8.
11. Docke WD, Randow F, Syrbe U, Krausch D, Asadullah K, Reinke P, et al. Monocyte deactivation in septic patients: restoration by IFN-gamma treatment. Nat Med 1997;3:678-81.
12. Wolk K, Hoflich C, Zuckermann-Becker H, Docke WD, Volk HD, Sabat R. Reduced monocyte CD86 expression in postinflammatory immunodeficiency. Crit Care Med 2007;35:458-67.
13. Docke WD, Hoflich C, Davis KA, Rottgers K, Meisel C, Kiefer P, et al. Monitoring temporary immunodepression by flow cytometric measurement of monocytic HLA-DR expression: a multicenter standardized study. Clin Chem 2005;51:2341-7.
14. Tolkoff-Rubin NE, Rubin RH. The interaction of immunosuppression with infection in the organ transplant recipient. Transplant Proc 1994;26:16-9.
15. Volk HD, Reinke P, Docke WD. Clinical aspects: from systemic inflammation to 'immunoparalysis'. Chem Immunol 2000;74:162-77.
16. Pallister CJ, Hancock JT. Phagocytic NADPH oxidase and its role in chronic granulomatous disease. Br J Biomed Sci 1995;52:149-56.
17. Philipp S, Wolk K, Kreutzer S, Wallace E, Ludwig N, Roewert J, et al. The evaluation of psoriasis therapy with biologics leads to a revision of the current view of the pathogenesis of this disorder. Expert Opin Ther Targets 2006;10:817-31.