Allosteric and Electrostatic Protein-Protein Interactions Regulate the Assembly of the Heterohexameric Tim9-Tim10 Complex

Journal of Molecular Biology

Volumn 379, Issue 3, 2008, Pages 609-616

  Ivanova, Ekaterina ^a   Lu, Hui ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

kinetics; pH dependence; protein protein interactions; salt effect; stopped flow fluorescence

Indexed keywords

CELL PROTEIN; PROTEIN TIM10; PROTEIN TIM9;

ALLOSTERISM; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ELECTRICITY; MITOCHONDRIAL MEMBRANE; MUTAGENESIS; NONHUMAN; PH MEASUREMENT; PKA; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PROTEIN INTERACTION;

EID: 43449100066     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2008.04.029     Document Type: Article

References (25)

1. I.M. Nooren J.M. Thornton Diversity of protein–protein interactions EMBO J. 22 2003 3486 3492
2. K. Henzler-Wildman D. Kern Dynamic personalities of proteins Nature 450 2007 964 972
3. H. Lu S. Allen L. Wardleworth P. Savory K. Tokatlidis Functional TIM10 chaperone assembly is redox-regulated in vivo J. Biol. Chem. 279 2004 18952 18958
4. H. Lu A.P. Golovanov F. Alcock J.G. Grossmann S. Allen L.Y. Lian K. Tokatlidis The structural basis of the TIM10 chaperone assembly J. Biol. Chem. 279 2004 18959 18966
5. S. Vial H. Lu S. Allen P. Savory D. Thornton J. Sheehan K. Tokatlidis Assembly of Tim9 and Tim10 into a functional chaperone J. Biol. Chem. 277 2002 36100 36108
6. C.T. Webb M.A. Gorman M. Lazarou M.T. Ryan J.M. Gulbis Crystal structure of the mitochondrial chaperone TIM9.10 reveals a six-bladed alpha-propeller Mol. Cell 21 2006 123 133
7. N. Pfanner A. Geissler Versatility of the mitochondrial protein import machinery Nat. Rev. Mol. Cell. Biol. 2 2001 339 349
8. 3C motif Trends Biochem. Sci. 29 2004 1 4
9. N. Wiedemann N. Pfanner A. Chacinska Chaperoning through the mitochondrial intermembrane space Mol. Cell 21 2006 145 148
10. M.F. Bauer U. Rothbauer N. Muhlenbein R.J. Smith K. Gerbitz W. Neupert The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom FEBS Lett. 464 1999 41 47
11. I.E. Gentle A.J. Perry F.H. Alcock V.A. Likic P. Dolezal E.T. Ng Conserved motifs reveal details of ancestry and structure in the small TIM chaperones of the mitochondrial intermembrane space Mol. Biol. Evol. 24 2007 1149 1160
12. H. Lu J. Woodburn Zinc binding stabilizes mitochondrial Tim10 in a reduced and import-competent state kinetically J. Mol. Biol. 353 2005 897 910
13. E. Ivanova M. Ball H. Lu Zinc binding of Tim10: evidence for existence of an unstructured binding intermediate for a zinc finger protein Proteins: Struct. Funct. Bioinf. 71 2008 467 475
14. B. Morgan H. Lu Oxidative folding competes with mitochondrial import of the small Tim proteins Biochem. J. 411 2008 115 122
15. E. Ivanova T.A. Jowitt H. Lu Assembly of the mitochondrial Tim9–Tim10 complex: a multi-step reaction with novel intermediates J. Mol. Biol. 375 2008 229 239
16. N.C. Price R.A. Dwek R.G. Ratcliffe M.R. Wormald Principles and Problems in Physical Chemistry for Biochemists 2006 Oxford University Press Oxford, UK 213 236
17. A. Fersht Structure and Mechanism in Protein Science: A Guide to Enzyme Catalysis and Protein Folding 1999 W.H. Freeman and Company New York, NY 289 323
18. D.S. Goodsell A.J. Olson Structural symmetry and protein function Annu. Rev. Biophys. Biomol. Struct. 29 2000 105 153
19. D.E. Koshland Application of a theory of enzyme specificity to protein synthesis Proc. Natl Acad. Sci. USA 44 1958 98 104
20. B. Ma S. Kumar C.J. Tsai R. Nussinov Folding funnels and binding mechanisms Protein Eng. 12 1999 713 720
21. C.S. Goh D. Milburn M. Gerstein Conformational changes associated with protein–protein interactions Curr. Opin. Struct. Biol. 14 2004 104 109
22. K.A. Dill H.S. Chan From Levinthal to pathways to funnels Nat. Struct. Biol. 4 1997 10 19
23. C.M. Dobson M. Karplus The fundamentals of protein folding: bringing together theory and experiment Curr. Opin. Struct. Biol. 9 1999 92 101
24. S.E. Radford Protein folding: progress made and promises ahead Trends Biochem. Sci. 25 2000 611 618
25. M.A. Vergnolle F.H. Alcock N. Petrakis K. Tokatlidis Mutation of conserved charged residues in mitochondrial TIM10 subunits precludes TIM10 complex assembly, but does not abolish growth of yeast cells J. Mol. Biol. 371 2007 1315 1324