Evaluation of a rapid polymerase chain reaction based identification technique for Vibrio cholerae isolates

Water Science and Technology

Volumn 50, Issue 1, 2004, Pages 229-232

  le Roux, W J ^a   Masoabi, D ^b   de Wet, C M E ^b   Venter, S N ^a  

^a UNIVERSITY OF PRETORIA   (South Africa)

^b Rand Water   (South Africa)

Author keywords

PCR; Phenotypic identificaton; Vibrio cholerae

Indexed keywords

BIOCHEMISTRY; DATABASE SYSTEMS; ENZYMES; HEALTH CARE; VIRUSES;

HEALTH PROTECTION; POLYMERASE;

WATER QUALITY;

BACTERIAL PROTEIN; DNA 16S; OUTER MEMBRANE PROTEIN;

IDENTIFICATION METHOD; PATHOGEN; POLYMERASE CHAIN REACTION; RESOURCE MANAGEMENT; WATERBORNE DISEASE;

ACCURACY; AEROMONAS; ANALYTIC METHOD; ARTICLE; AUTOMATION; BACTERIAL GENE; BACTERIUM CONTAMINATION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CHEMICAL ANALYSIS; CONTROLLED STUDY; DNA SEQUENCE; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC DATABASE; INTERMETHOD COMPARISON; INTERNET; NONHUMAN; PHENOTYPIC VARIATION; POLYMERASE CHAIN REACTION; RELIABILITY; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY; VIBRIO CHOLERAE; WATER CONTAMINATION; WATER SAMPLING;

AEROMONAS; VIBRIO; VIBRIO CHOLERAE;

EID: 4344703991     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2004.0059     Document Type: Article

References (8)

1. Farmer III, J.J. and Hickman-Brenner, F.W. (1992). The genera Vibrio and Photobacterium. In: The Prokaryotes, A. Balows et al. (eds), Springer-Verlag, New York, pp. 2952-3011.
2. Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. (1994). Bergey's Manual of Systematic Bacteriology, 9th edn, Williams and Wilkins, Baltimore.
3. Kobayashi, T., Enomoto, S., Sakuzaki, R. and Kuwahara, S. (1963). A new selective isolation medium for the Vibrio group, on modified Nakanishi's medium. Jpn J. Bact., 18, 387-392.
4. Nandi, B., Nandy, R.K., Mukhopadhyay, S., Nair, G.B., Shimada, T. and Ghose, A. (2000). Rapid method for species-specific identification of V. cholerae using primers targeted to the outer membrane protein. J. Clin. Microbiol., 38, 4145-4151.
5. Saiki, R.K., Gelfand, D.H. and Stoffel, S. (1988). Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 487-491.
6. Theron, J., Cilliers, J., du Preez, M., Brozel, V.S. and Venter, S.N. (2000). Detection of toxigenic V. cholerae from environmental water samples by an enrichment broth cultivation pit-stop semi-nested PCR procedure. Appl. Env. Microbiol., 89, 539-546.
7. Weisburg, W.G., Barns, S.M., Pelletier, D.A. and Lane, D.J. (1991). 16S Ribosomal DNA amplification for phylogenetic study. J. Bacteriol., 173, 697-703.
8. WHO (2002). Cholera 2001. WHO Wkly. Epidemiol. Rec., 77,257-268.