Combining direct viable count (DVC) and fluorescent in situ hybridisation (FISH) to enumerate viable E. coli in rivers and wastewaters

Water Science and Technology

Volumn 50, Issue 1, 2004, Pages 271-275

  Garcia Armisen, T ^a   Servais, P ^a  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

Author keywords

E. coli; Flourescent in situ hybridisation (FISH); Rivers; Wastewaters

Indexed keywords

BACTERIA; CELLS; FLUORESCENCE; MICROSCOPIC EXAMINATION; RNA;

DIRECT VIABLE COUNT (DVC) PROCEDURE; EPIFLUORESCENCE MICROSCOPY;

WATER QUALITY;

ANTIBIOTIC AGENT; RIVER WATER; RNA 16S;

COLIFORM BACTERIUM; FLUORESCENCE; RIVER WATER; WASTEWATER;

ACCURACY; ANALYTICAL PARAMETERS; ARTICLE; BACTERIAL CELL; BACTERIAL COUNT; BACTERIAL SURVIVAL; BACTERIUM CULTURE; BACTERIUM DETECTION; CELL DIVISION; CELL ELONGATION; CELL VIABILITY; CONTROLLED STUDY; CORRELATION ANALYSIS; CULTURE MEDIUM; ENVIRONMENTAL MONITORING; EPIFLUORESCENCE MICROSCOPY; ESCHERICHIA COLI; EXPOSURE; FLUORESCENCE IN SITU HYBRIDIZATION; INTERMETHOD COMPARISON; MOLECULAR PROBE; NONHUMAN; RIVER; WASTE WATER; WATER SAMPLING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 4344638145     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2004.0066     Document Type: Article

References (7)

1. Colwell, R.R. et al. (1985). Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for release of genetically engineered microorganisms. Biotechnology, 3, 817-820.
2. Delabre, K. et al. (2001). New analytical tools for distribution system surveillance. In: Proceedings of AWWA-Annual Conference, Washington, D.C., CD-Rom.
3. Edberg, S.C., Allen, M.J. and Smith, D.B. (1989). National field evaluation of a defined substrate method for the simultaneous enumeration of total coliforms and Escherichia coli from drinking water: comparison with the standard multiple tube fermentation method. Appl. Environ. Microbiol., 54, 1595-1601.
4. Grimes, D.J.R. and Colwell, R. (1986). Viability and virulence of Escherichia coli suspended by membrane chamber in semitropical ocean water. FEMS Microbiol. Lett., 34, 161-165.
5. Kogure, K., Simidu, U. and Taga, N. (1979). A tentative direct microscopic method for counting living marine bacteria. Can. J. Microbiol., 25, 415-420.
6. Pommepuy, M. et al. (1996). Retention of enteropathogenicity by viable but nonculturable Escherichia coli exposed to seawater and sunlight. Appl. Environ. Microbiol., 62, 4621-4626.
7. Regnault, B. et al. (2000). Oligonucleotide probe for the visualization of Escherichia coli/Escherichia fergusonii cells by in situ hybridization: specificity and potential application. Res. Microbiol., 151, 521-533.