Identification of genes involved in VEGF-mediated vascular morphogenesis using embryonic stem cell-derived cystic embryoid bodies

Laboratory Investigation

Volumn 84, Issue 9, 2004, Pages 1209-1218

  Ng, Yin Shan ^a,b   Ramsauer, Markus ^a   Loureiro, Robyn M B ^a   D'Amore, Patricia A ^a  

^a SCHEPENS EYE RESEARCH INSTITUTE   (United States)

^b Eyetech Pharmaceuticals Inc   (United States)

Author keywords

Angiogenesis; Branching; Differentiation; Lumen; Microarray; Sprouting; Vasculogenesis

Indexed keywords

VASCULOTROPIN;

ANGIOGENESIS; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; EMBRYO CELL; ENDOTHELIUM CELL; EXPRESSED SEQUENCE TAG; GENE EXPRESSION; GENE IDENTIFICATION; IN VITRO STUDY; KNOCKOUT GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; STEM CELL; VASCULARIZATION;

ANIMALS; CAVEOLIN 1; CAVEOLINS; CELL CYCLE PROTEINS; CELLS, CULTURED; EMBRYO; EMBRYONIC AND FETAL DEVELOPMENT; GENE EXPRESSION REGULATION, DEVELOPMENTAL; IMMUNOHISTOCHEMISTRY; MICE; MICE, KNOCKOUT; MODELS, ANIMAL; NEOVASCULARIZATION, PHYSIOLOGIC; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; STEM CELLS; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 4344613569     PISSN: 00236837     EISSN: None     Source Type: Journal    
DOI: 10.1038/labinvest.3700150     Document Type: Article

References (24)

1. Carmeliet P, Ferriera V, Breier G, et al. Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele. Nature 1996;380:435-439.
2. Ferrara N, Carver-Moore K, Chen H, et al. Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene. Nature 1996;380:439-442.
3. Baatout S. Endothelial differentiation using Matrigel. Anticancer Res 1996;17:451-455.
4. Davis G, Camarillo C. An alpha 2 beta 1 integrin-dependent pinocytotic mechanism involving intracellular vacuole formation and coalescence regulates capillary lumen and tube formation in three-dimensional collagen matrix. Exp Cell Res 1996;10:39-51.
5. Evans M, Kaufman M. Establishment in culture of pluripotential cells from mouse embryos. Nature 1981;292:154-156.
6. Martin G. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci USA 1981;78:7634-7638.
7. Krah K, Mironov V, Risau W, et al. Induction of vasculogenesis in quail blastodisc-derived embryoid bodies. Dev Bio 1994;164:123-132.
8. Risau W, Sariola H, Zerwes HG, et al. Vasculogenesis and angiogenesis in embryonic-stem-cell-derived embryoid bodies. Development 1988;102:471-478.
9. Bautch VL, Stanford WL, Rapoport R, et al. Blood island formation in attached cultures of murine embryonic stem cells. Dev Dyn 1996;205:1-12.
10. Desbaillets I, Ziegler U, Groscurth P, et al. Embryoid bodies: an in vitro model of mouse embryogenesis. Exp Physiol 2000;85:645-651.
11. Feraud O, Cao Y, Vittet D. Embryonic stem cell-derived embryoid bodies development in collagen gels recapitulates sprouting angiogenesis. Lab Invest 2001;81: 1669-1681.
12. Dieffenbach C, Dveksler G. PCR Primer: A Laboratory Manual. Cold Spring Harbor Laboratory Press: New York, 1995.
13. Shima DT, Deutsch U, D'Amore PA. Hypoxic induction of vascular endothelial growth factor (VEGF) in human epithelial cells is mediated by increases in mRNA stability. FEBS Lett 1995;370:203-208.
14. Ikeda E, Achen MG, Brier G, et al. Hypoxia-induced transcriptional activation and increased messenger RNA stability of vascular endothelial growth factor in C6 glioma cells. J Biol Chem 1995;270:9761-9766.
15. Stein I, Neeman M, Shweiki D, et al. Stabilization of vascular endothelial growth factor mRNA by hypoxia and hypoglycemia and coregulation with other ischemia-induced genes. Mol Cell Biol 1995;15:5363-5368.
16. Miquerol L, Gertsenstein M, Harpal K, et al. Multiple developmental roles of VEGF suggested by a LacZ-tagged allele. Dev Biol 1999;212:307-322.
17. Griffoni C, Spisni E, Santi S, et al. Knockdown of caveolin-1 by antisense oligonucleotides impairs angiogenesis in vitro and in vivo. Biochem Biophys Res Commun 2000;276:756-761.
18. Woodman SE, Ashton AW, Schubert W, et al. Caveolin-1 knockout mice show an impaired angiogenic response to exogenous stimuli. Am J Pathol 2003;162: 2059-2068.
19. Liu J, Wang XB, Park DS, et al. Caveolin-1 expression enhances endothelial capillary tubule formation. J Biol Chem 2002;277:10661-10668.
20. Rahimi N, Kazlauskas A. A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells. Mol Biol Cell 1999;10:3401-3407.
21. Henderson AM, Wang SJ, Taylor AC, et al. The basic helix-loop-helix transcription factor HESR1 regulates endothelial cell tube formation. J Biol Chem 2001; 276:6169-6176.
22. Ruhrberg C, Gerhardt H, Golding M, et al. Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesis. Genes Dev 2002;16:2684-2698.
23. Deng C, Bedford M, Li C, et al. Fibroblast growth factor receptor-1 (FGFR-1) is essential for normal neural tube and limb development. Dev Biol 1997;185: 42-54.
24. Soudais C, Bielinska M, Heikinheimo M, et al. Targeted mutagenesis of the transcription factor GATA-4 gene in mouse embryonic stem cells disrupts visceral endoderm differentiation in vitro. Development 1995;121:3877-3888.