Molecular and Cytogenetic Characterization of Triploid Somatic Hybrids between 'Shogun' Mandarin and Grapefruit

Plant Biotechnology

Volumn 19, Issue 5, 2002, Pages 345-352

  Kunitake, Hisato ^a   Nagasawa, Kohji ^b,c   Komatsu, Haruki ^b  

^a UNIVERSITY OF MIYAZAKI   (Japan)

^b TOKAI UNIVERSITY   (Japan)

^c Hakusan trading Co Ltd   (Japan)

Author keywords

Citrus; electrofusion; flow cytometry; PCR RFLP; somatic hybrid; triploid

Indexed keywords

EID: 4344583477     PISSN: 13424580     EISSN: 13476114     Source Type: Journal    
DOI: 10.5511/plantbiotechnology.19.345     Document Type: Article

References (31)

1. Arumuganathan, K., Earle, E. D., 1991. Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol. Biol. Rep., 9: 229-233.
2. Buiteveld, J., Suo, M. M. van Lookerren C. Creemer-Molenaar, J., 1998. Production and characterization of somatic hybrid plants between leek (Allium ampelo-prasum L.) and onion (A. cepa L.). Theor. Appl. Genet., 96: 765-775.
3. D' Amato., 1995. Cytogenetics of plant cell and tissue cultures and their regenerates. CRC Citical Reviews in Plant Sciences, pp. 73-112.
4. Doyle, J., Doyle, J. L., 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull., 19: 11-15.
5. Grosser, J. W., Gmitter, F. G., Jr Sesto, F., Deng, X. X., Chandler, J. L., 1992. Six new somatic hybrids and their potential for cultivar improvement. J. Amer. Soc. Hort. Sci., 117: 169-173.
6. Grosser, J. W., Mourao-Fo, F. A. A., Gmitter, Jr, FGL-louzada, E. S., Jiang, J., Baergen, K., Quiros, A., Cabasson, C., Schell, J. L., Chandler, J. L., 1996. Allotetraploid hybrids between Citrus and seven related genera produced by somatic hybridization. Theor. Appl. Genet., 92: 577-582.
7. Guo, W. W., Deng, X. X., 1999. Intertribal hexaploid somatic hybrid plants regeneration from elevtrofusion between diploids of Citrus sinensis and its sexually incompatible relative, Clausena lansium. Theor. Appl. Genet., 98: 581-585.
8. Guo, W. W., Cheng, Y. J., Deng, X. X., 2002. Regeneration and molecular characterization of intergeneric somatic hybrids between Citrus reticulata and Poncirus trifoliata. Plant Cell Rep., 20: 829-834.
9. Haruki, K., Hosoki, T., Nako, Y., 1998. Tracing the parentages of some oriental hybrid lily cultivars by PCR-RFLP analysis. J. Japan, Soc. Hort. Sci., 67: 352-359.
10. Harusaki, S., Kokuryo, D., Kunitake, H., Komatsu, H., 2000. Determination of ploidy levels of Citrus species using flow cytometry. Proc, Sch. Agri, Kyushu Tokai Univ., 19: 45-52.
11. Kobayashi, S., Ohgawara, T., Fujiwara, K., Oiyama, I., 1991. Analysis of cytoplasmic genomes in somatic hybrids between navel orange (Citrus sinensis Osb.) and 'Murcott'tangor. Theor. Appl. Genet., 82: 6-10.
12. Kobayashi, S., Ohgawara, T., Saito, W., Nakamura, Y., Omura, M., 1995. Fruit characteristics and pollen fertility of Citrus somatic hybrids. J. Japan. Soc. Hort. Sci., 64: 283-289.
13. Kobayashi, S., Ohgawara, T., Saito, W., Nakamura, Y., Omura, M., 1997. Production of triploid somatic hybrids in Citrus. J. Japan. Hort. Sci., 66: 453-458.
14. Kunitake, H., Kagami, H., Mii, M., 1991. Somatic embryogenesis and plant regeneration from protoplasts of Satsuma mandarin. Sci. Hortic., 47: 27-33.
15. Mendes, B. M. J., Filho, F. A., Farias, P. M., Benedito, V. A., 2001. Citrus somatic hybridization with potential for improved bright and CTV resistance. In Vitro Cell. Dev. Biol, 37: 490-495.
16. Miranda, M., Motomura, T., Ikeda, F., Ohgawara, T., Saito, W., Endo, T., Omura, M., Moriguchi, T., 1997. Somatic hybrids obtained by fusion between Poncirus trifoliata (2x) and Fortunella hindsii (4x) protoplasts. Plant Cell Rep., 16: 401-405.
17. Murashige, T. Tucker, D. P. H., 1969. Growth factor requirements of Citrus tissue culture. Proc. 1 st Int. Citrus Symp., pp. 1155-1161.
18. Nuti-Ronchi, V., Giorgetti, L., Tonelli, M., Martini, G., 1992. Ploidy reduction and genome segregation in cultured carrot cell lines. I. Prophase chromosome reduction. Plant Cell, Tissue Organ Cult., 30: 107-114.
19. Ogihara, Y., Terachi, T., Sasakuma, T., 1991. Molecular analysis of the hot spot region related to length mutations in wheat chloroplast DNAs. I. Nucleotide divergence of genes and intergenic spacer regions located in the hot region. Genetics, 129: 873-884.
20. Ohgawara, T., Kobayashi, S., Ohgawara, E., Uchimiya, H., Ishii, S., 1985. Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theor. Appl. Genet., 71: 1-4.
21. Ohgawara, T., Kobayashi, S., Ishii, S., Yoshinaga, K., Oiyama, I., 1989. Somatic hybridization in Citrus: navel orange (C. sinensis Osb.) and grapefruit (C. paradisi Macf.). Theor. Appl. Genet., 78: 609-612.
22. Oiyama, I., 1981. A technique for chromosome observation in root tip cells of Citrus. Bull. Fruit Tree Res. Sta., D 3: 1-7.
23. Ollitrault, P. D., Dambier, D., Jacquemond, C., Allent, V., Luro, F., 1996. In vitro rescue and selection of spontaneous triploids by flow cytometry for easy peeler Citrus breeding. Proc. Int. Soc. Citricult, pp. 254-258.
24. Ollitrault, P. D., Dambier, D., Sudahono, Mademba-Sy, F., Vanel, F., Luro, F., 1998. Biotechnology for triploid mandarin breeding. Fruits, 53: 307-317.
25. Shoost, R. K., Cameron, J. W., 1980. 'Orobalanco', triploid pummelo-grapefruit hybrid. HortSci., 15: 667-669.
26. Shoost, R. K., Cameron, J. W., 1985. 'Melogold', triploid pummelo-grapefruit hybrid. HortSci., 20: 1134-1135.
27. Singh, B. D., Harvey, R. L., Kao, K. N., Miller, R. A., 1972. Selection pressure in cell population of Vicia hajastana cultured in vitro. Can. J. Genet. Cytol., 14: 65.
28. Tusa, N., Fatta Del Bosco, S., Nardi, L., Lucretti, S., 1996. Obtaining triploid plants by crossing Citrus lemon cv. 'Femineilo'2N X 4N allotetraploid somatic hybrids. Proc. Int. Soc. Citricult., pp. 133-136.
29. Widholm, J. M., 1972. The use of fluorescein diacetate and phenosafranin for determining viability of cultured plant cells. Stain. Tech., 47: 189-194.
30. Williams, J. G. K., Kubelik, A. E., Levak, K. J., Rafalski, J. A., Tingey, S. C., 1990. DNA polymorphisms amplified by arbitary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531-6535.
31. Xu, Y., Clark, MS., Pehu, E., 1993. Use of RAPD makers to screen somatic hybrids between Solanum tuberosum andS. brevidens. Plant Cell Rep., 12: 107-109.