Pretreatment of human epidermal keratinocytes in vitro with ethacrynic acid reduces sulfur mustard cytotoxicity

Toxicology Mechanisms and Methods

Volumn 14, Issue 5, 2004, Pages 317-322

  Gross, Clark L ^a   Nipwoda, Mary T ^a   Nealley, Eric W ^a   Smith, William J ^a  

^a US ARMY MEDICAL RESEARCH INSTITUTE OF CHEMICAL DEFENSE   (United States)

Author keywords

Cytotoxicity; Ethacrynic Acid; Pretreatment; Sulfur Mustard

Indexed keywords

ACETYLCYSTEINE; ALKYLATING AGENT; BUTHIONINE SULFOXIMINE; ETACRYNIC ACID; GLUTATHIONE; GLUTATHIONE TRANSFERASE; MUSTARD GAS; OLTIPRAZ;

ARTICLE; CELL SURVIVAL; CELL VIABILITY; CONJUGATE; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ACTIVITY; EPIDERMIS; HUMAN; HUMAN CELL; KERATINOCYTE; PRIORITY JOURNAL;

MAMMALIA;

EID: 4344572211     PISSN: 15376516     EISSN: 15376524     Source Type: Journal    
DOI: 10.1080/15376520490434601     Document Type: Article

References (10)

1. Black, R. M., Brewster, K., Clarke, R. J., Hambrook, J. L., Harrison, J. M., and Howells, D. J. 1992. Biological fate of sulphur mustard, 1,1′-thiobis(2-chloroethane): Isolation and identification of urinary metabolites following intraperitoneal administration to rat. Xenobiotica 22(4):405-418.
2. Chen, G., and Waxman, D. J. 1994. Role of cellular glutathione and glutathione S-transferase in the expression of alkylating agent cytotoxicity in human breast cancer cells. Biochem. Pharm. 47(6):1079-1087.
3. Davison, C., Rozman, R. S., and Smith, P. K. 1961. Metabolism of bis-β-chloroethyl sulfide (sulfur mustard gas). Biochem. Pharm. 7:65-74.
4. GarciaAlfonso, C., Repetto, G., Sanz, P., Repetto, M., and LopezBarea, J. 1998. Direct determination of glutathione-S-transferase and glucose-6-dehydrogenase activities in cells cultured in microtitre plates as biomarkers for oxidative stress. ATLA-Alternatives to Laboratory Animals 26(3):321-330.
5. Griffith, O. W. 1980. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal. Biochem. 106:207-212.
6. Gross, C. L., Giles, K. C., and Smith, W. J. 1997. L-Oxothiazolidine 4-carboxylate pretreatment of isolated human peripheral blood lymphocytes reduced sulfur mustard cytotoxicity. Cell Biol. Toxicol. 13:167-173.
7. Gross, C. L., Innace, J. K., Hovatter, R. C., Meier, H. L., and Smith, W. J., 1993. Biochemical manipulation of intracellular glutathione levels influences cytotoxicity to isolated human lymphocytes by sulfur mustard. Cell Biol. Toxicol. 9:259-267.
8. Papirmeister, B., Feister, A. J., Robinson, S. I., and Ford, R. D. 1991. Medical Defense Against Mustard Gas: Toxic Mechanisms and Pharmacological Implications, CRC Press: Boca Raton, Florida.
9. Ploemen, J. H. T. M., Van Ommen, B., and Van Bladeren, P. J. 1990. Inhibition of rat and human glutathione-S-transferase isoenzymes by ethacrynic acid and its glutathione conjugate. Biochem. Pharm. 40(7):1631-1635.
10. Roberts, J. J., and Warwick, G. P. 1963. Studies of the mode of action of alkylating agents VI: The metabolism of bis-β chloroethylsulfide (mustard gas) and related compounds. Biochem. Pharm. 12:1329-1334.