Polyclonal versus monoclonal ELISA for the determination of fecal elastase 1: Diagnostic value in cystic fibrosis and chronic pancreatic insufficiency

Clinical Laboratory

Volumn 50, Issue 7-8, 2004, Pages 419-424

  Miendje, Yvette ^a,b   Maisin, Diane ^a   Sipewa, Marie J ^a   Deprez, Pierre ^a   Buts, Jean P ^a   De Nayer, Philippe ^a   Philippe, Marianne ^a  

^a CLINIQUES UNIVERSITAIRES SAINT LUC   (Belgium)

^b BRUGMANN UNIVERSITY HOSPITAL   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

MONOCLONAL ANTIBODY; PANCREATIC ELASTASE; POLYCLONAL ANTIBODY;

ADOLESCENT; ADULT; ARTICLE; CHILD; CHRONIC PANCREATITIS; CONTROLLED STUDY; CYSTIC FIBROSIS; DIAGNOSTIC KIT; DIAGNOSTIC PROCEDURE; DIAGNOSTIC VALUE; DISEASE SEVERITY; ENZYME ASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES ANALYSIS; GASTROINTESTINAL DISEASE; HUMAN; INFANT; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; PANCREAS CALCIFICATION; PANCREAS FUNCTION; PANCREAS INSUFFICIENCY; SENSITIVITY AND SPECIFICITY; STATISTICAL SIGNIFICANCE;

ADULT; ANTIBODIES; CHILD; CYSTIC FIBROSIS; DIAGNOSTIC TECHNIQUES, ENDOCRINE; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXOCRINE PANCREATIC INSUFFICIENCY; FECES; HUMANS; PANCREATIC ELASTASE; REAGENT KITS, DIAGNOSTIC;

EID: 4344566979     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Stein J, Jung M, Sziegolet A, Zeuzem S, Caspary WF, Lembcke B. Immunoreactive elastase I: clinical evaluation of a new non-invasive test of pancreatic function. Clin Chem 1996;42:222-6.
2. Sziegolet A, Krause E, Klor HU, Kanacher L, Linder D. Elastase 1 and chymotrypsin B in pancreatic juice and feces. Clin Biochem 1989;22:85-9.
3. Katschinski M, Schirra J, Bross A, Arnold R, Göke B. Fecal concentration of pancreatic elastase 1 accurately indicates pancreatic exocrine insufficiency. Gastroenterology 1994; 106:A79.
4. Stein J, Jung M, Zeuzem S, Lembcke B, Caspary WF. Fecal elastase-1: A new tubeless test in the diagnosis of pancreatic insufficiency. Gastroenterology 1994;106:A325.
5. Löser C, Mölgaard A, Fölsch UR. Elastase 1 in faeces: A novel highly sensitive and specific pancreatic function test for easy and inexpensive routine application. Digestion 1995;56:301.
6. Walkoviak J, Cichy WK, Herzig KH. Comparison of fecal elastase-1 determination with the secretin-cholecystokinin test in patients with cystic fibrosis. Scandinavian Journal of Gastroenterology 1999;34:201-7.
7. Sziegoleit A, Linder D. Studies on the sterol-binding capacity of human pancreatic elastase. Gastroenterology 1991; 100:768-74.
8. Scheefers-Borchel U, Scheefers H, Arnold R, Fischer P, Sziegoleit A. Pancreatische Elastase 1: Parameter für die chronische und akute Pankreatitis. Lab med 1992;16:427-32.
9. Dominguez-Munoz JE, Hieronymus C, Sauerbruch T, Malfertheiner P. Fecal elastase test (FET) in the diagnosis and staging of chronic pancreatitis. Digestion 1994;55:292-3.
10. Glasbrenner B, Beckh KH, Adler G. Comparison of fecal elastase 1 with other indirect pancreatic function tests. Digestion 1994;55:301-2.
11. Soldan W, Henker J, Sprössig Christa. Sensitivity and specificity of quantitative determination of pancreatic elastase 1 in feces of children. Journal of Pediatric Gastroenterology and Nutrition 1997;24:53-55.
12. Deffert C, Chouraqui JP, Girardet JP, Hubert D, Pressac M. Intérêt du dosage de l'élastase pancréatique fécale chez les sujets mucoviscidosiques. Immunoanalyse 2000; 15:427-31.
13. Hardt PD, Hauenschild A, Nalop J, Marzeion AM, Porsch-Ozcurumez M, Luley C, Sziegoleit A, Kloer HU. The commercially available ELISA for pancreatic elastase 1 based on polyclonal antibodies does measure an as yet unknown antigen different from purified elastase 1. Binding studies and clinical use in patients with exocrine pancreatic insufficiency. Z Gastroenterol. 2003;41(9):903-6.
14. Keim V, Teich N, Moessner J. Clinical value of a new fecal elastase test for the detection of chronic pancreatitis. Clin. Lab. 2003;49:209-15.