Exploring the active site cavity of human pancreatic lipase

Biochemical and Biophysical Research Communications

Volumn 370, Issue 3, 2008, Pages 394-398

  Colin, Damien Yann ^a,b,c   Deprez Beauclair, Paule ^a,b,c   Allouche, Maya ^a,b,c   Brasseur, Robert ^d   Kerfelec, Brigitte ^a,b,c  

^a INSERM   (France)

^b INSERM   (France)

^c AIX MARSEILLE UNIVERSITY   (France)

^d UNIVERSITY OF LIÈGE   (Belgium)

Author keywords

Colipase; Cystic fibrosis; Pancreatic lipase; pH activity profile

Indexed keywords

COLIPASE; HISTIDINE; TRIACYLGLYCEROL LIPASE;

ACIDITY; ARTICLE; CATALYSIS; CHARGE; CYSTIC FIBROSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME THERAPY; HUMAN; NONHUMAN; PARAMETER; PH; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

EID: 43049165783     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.03.043     Document Type: Article

References (23)

1. K. De Boeck I. Delbeke E. Eggermont G. Veereman-Wauters Y. Ghoos Lipid digestion in cystic fibrosis: comparison of conventional and high-lipase enzyme therapy using the mixed-triglyceride breath test J. Pediatr. Gastroenterol. Nutr. 26 1998 408 411
2. M. Barraclough C.J. Taylor Twenty-four hour ambulatory gastric and duodenal pH profiles in cystic fibrosis: effect of duodenal hyperacidity on pancreatic enzyme function and fat absorption J. Pediatr. Gastroenterol. Nutr. 23 1996 45 50
3. B. Borgstrom On the interactions between pancreatic lipase and colipase and the substrate, and the importance of bile salts J. Lipid Res. 16 1975 411 417
4. F.K. Winkler A. D’Arcy W. Hunziker Structure of human pancreatic lipase Nature 343 1990 771 774
5. J. Hermoso D. Pignol B. Kerfelec I. Crenon C. Chapus J.C. Fontecilla-Camps Lipase activation by nonionic detergents. The crystal structure of the porcine lipase–colipase–etraethylene glycol monooctyl ether complex J. Biol. Chem. 271 1996 18007 18016
6. M.P. Egloff F. Marguet G. Buono R. Verger C. Cambillau H. van Tilbeurgh The 2.46 Å resolution structure of the pancreatic lipase-colipase complex inhibited by a C11 alkyl phosphonate Biochemistry 34 1995 2751 2762
7. C. Chaillan B. Kerfelec E. Foglizzo C. Chapus Direct involvement of the C-terminal extremity of pancreatic lipase (403–449) in colipase binding Biochem. Biophys. Res. Commun. 184 1992 206 211
8. M.E. Lowe The catalytic site residues and interfacial binding of human pancreatic lipase J. Biol. Chem. 267 1992 17069 17073
9. S. Bezzine F. Ferrato M.G. Ivanova V. Lopez R. Verger F. Carriere Human pancreatic lipase: colipase dependence and interfacial binding of lid domain mutants Biochemistry 38 1999 5499 5510
10. A. Thomas M. Allouche F. Basyn R. Brasseur B. Kerfelec Role of the lid hydrophobicity pattern in pancreatic lipase activity J. Biol. Chem. 280 2005 40074 40083
11. M.L. Jennens M.E. Lowe A surface loop covering the active site of human pancreatic lipase influences interfacial activation and lipid binding J. Biol. Chem. 269 1994 25470 25474
12. L. Ayvazian I. Crenon J. Hermoso D. Pignol C. Chapus B. Kerfelec Ion pairing between lipase and colipase plays a critical role in catalysis J. Biol. Chem. 273 1998 33604 33609
13. M.T. Neves Petersen P. Fojan S.B. Petersen How do lipases and esterases work: the electrostatic contribution J. Biotechnol. 85 2001 115 147
14. P. Ramos T. Coste E. Piemont J.M. Lessinger J.A. Bousquet C. Chapus B. Kerfelec G. Ferard Y. Mely Time-resolved fluorescence allows selective monitoring of Trp30 environmental changes in the seven-Trp-containing human pancreatic lipase Biochemistry 42 2003 12488 12496
15. H.A. Erlich PCR Technology: Principles and Applications for DNA Amplification 1992 W. H. Freeman and Co. New York
16. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
17. W.N. Burnette “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate–polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A Anal. Biochem. 112 1981 195 203
18. P. Desnuelle The Enzymes 1972 Academic Press Inc. New York, London
19. A. Thomas A. Milon R. Brasseur Partial atomic charges of amino acids in proteins Proteins 56 2004 102 109
20. A. Vandermeers M.C. Vandermeers-Piret J. Rathe J. Christophe Effect of colipase on adsorption and activity of rat pancreatic lipase on emulsified tributyrin in the presence of bile salt FEBS Lett. 49 1975 334 337
21. S. Granon M. Semeriva Effect of taurodeoxycholate, colipase and temperature on the interfacial inactivation of porcine pancreatic lipase Eur. J. Biochem. 111 1980 117 124
22. B.A. van Kuiken W.D. Behnke The activation of porcine pancreatic lipase by cis -unsaturated fatty acids Biochim. Biophys. Acta 1214 1994 148 160
23. C. Eydoux J. De Caro F. Ferrato P. Boullanger D. Lafont R. Laugier F. Carriere A. De Caro Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells J. Lipid Res. 48 2007 1539 1549