Comparative examination and validation of ELISA test systems for Salmonella typhimurium diagnosis of slaughtering pigs

International Journal of Food Microbiology

Volumn 124, Issue 1, 2008, Pages 65-69

  Szabo I ^a   Scherer, K ^a   Roesler, U ^b   Appel, B ^a   Nockler K ^a   Hensel, A ^a  

^a FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Cut off; ELISA; Pig; Salmonella typhimurium

Indexed keywords

IMMUNOGLOBULIN A ANTIBODY; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; LIPOPOLYSACCHARIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY DETECTION; ARTICLE; BLOOD SAMPLING; CELL LYSATE; COMPARATIVE STUDY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; FOOD CONTAMINATION; GERMANY; NONHUMAN; SALMONELLA TYPHIMURIUM; SALMONELLOSIS; SLAUGHTERHOUSE; SWINE; VALIDATION STUDY;

ABATTOIRS; ANIMALS; ANTIBODIES, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FOOD CONTAMINATION; HUMANS; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; LIPOPOLYSACCHARIDES; REPRODUCIBILITY OF RESULTS; SALMONELLA INFECTIONS, ANIMAL; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; SWINE; SWINE DISEASES;

SALMONELLA; SALMONELLA TYPHIMURIUM; SUIDAE;

EID: 43049161156     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2008.02.022     Document Type: Article

References (12)

1. Davies R.H., Heath P.J., Coxon S.M., and Sayers A.R. Evaluation of the use of pooled serum, pooled muscle tissue fluid (meat juice) and pooled faeces for monitoring pig herds for Salmonella. Journal of Applied Microbiology 95 (2003) 1016-1025
2. Ehlers J., Alt M., Trepnau D., and Lehmann J. Use of new immunoglobulin isotype specific ELISA-systems to detect Salmonella infections in pigs (German). Berliner und Münchener Tierärztliche Wochenschrift 119 (2006) 461-466
3. Lehmann J., Lindner T., Naumann M., Kramer T., Steinbach G., Blaha T., Ehlers J., Selbitz H.-J., Gabert J., and Roesler U. Application of a novel pig immunoglobulin-isotype-specific enzyme-linked immunoadsorbent assay for detection of Salmonella enterica serovar typhimurium antibodies in serum and meat juice. Proceedings of the 18th International Pig Veterinary Society World Congress, Hamburg vol. 1 (2004) 383
4. Mousing J., Jensen P.T., Halgaard C., Bager F., Feld N., Nielsen B., Nielsen J.P., and Bech-Nielsen S. Nation wide Salmonella enterica surveillance and control in Danish slaughter swineherds. Preventive Veterinary Medicine 29 (1997) 247-261
5. Nielsen B., Baggesen D., Bager F., Haugegaard J., and Lind P. The serological response to Salmonella serovar typhimurium and infantis in experimentally infected pigs. The time course followed with an indirect anti-LPS-ELISA and bacteriological examinations. Veterinary Microbiology 47 (1995) 205-218
6. Nielsen B., Ekeroth L., Bager F., and Lind P. Use of muscle fluid as a source of antibodies for serologic detection of Salmonella infection in slaughter pig herds. Journal of Veterinary Diagnostic Investigation 10 (1998) 158-163
7. Nollet N., Maes D., Duchateau L., Hautekiet V., Houf K., Van Hoof J., De Zuttera L., De Kruif A., and Geers R. Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs. Veterinary Research 36 (2005) 545-555
8. Roesler U., Marg H., Schröder I., Mauer S., Arnold T., Lehmann J., Truyen U., and Hensel A. Oral vaccination of pigs with an invasive gyrA-cpxA-rpoB Salmonella typhimurium mutant. Vaccine 23 (2004) 595-603
9. Steinbach G., and Kroell U. Salmonella infections in swine herds - epidemiology and importance for human illness (German). Deutsche Tierärztliche Wochenschrift 106 (1999) 269-308
10. Steinbach G., Methner U., and Meyer H. Distribution of immunglobuline isotypes and Salmonella antibodies in blood serum and meat juice of pigs. Deutsche Tierärztliche Wochenschrift 116 (2003) 274-280
11. van der Heijden H.M. First international ring trial of ELISAs for Salmonella-antibody detection in swine. Berliner und Münchener Tierärztliche Wochenschrift 114 (2001) 389-392
12. Wilhelm E., Hilbert F., Paulsen P., Smulders F.J.M., and Rossmanith W. Salmonella diagnosis in pig production: methodological problems in monitoring the prevalence in pigs and pork. Journal of Food Protection 70 (2007) 1246-1248