Crystallization of RNA polymerase I subcomplex A14/A43 by iterative prediction, probing and removal of flexible regions

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 64, Issue 5, 2008, Pages 413-418

  Geiger, Sebastian R ^a   Kuhn, Claus D ^a,b   Leidig, Christoph ^a   Renkawitz, Jörg ^a   Cramer, Patrick ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

Crystal engineering; Mapping unstructured regions; Multiprotein crystals; Protein engineering; RNA polymerase I; Rpb4 7 subcomplex

Indexed keywords

DNA DIRECTED RNA POLYMERASE; RNA POLYMERASE II; RPB7 PROTEIN, S CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOLOGY; CHEMICAL STRUCTURE; CHEMISTRY; CRYSTALLIZATION; DIMERIZATION; ENZYMOLOGY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RNA POLYMERASE I; RNA POLYMERASE II; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 43049145506     PISSN: 17443091     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S174430910800972X     Document Type: Article

References (17)

1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. (1997). Nucleic Acids Res. 25, 3389-3402.
2. Bergfors, T. (2003). J. Struct. Biol. 142, 66-76.
3. Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T. J., Higgins, D. G. & Thompson, J. D. (2003). Nucleic Acids Res. 31, 3497-3500.
4. Galtier, N., Gouy, M. & Gautier, C. (1996). Comput. Appl. Biosci. 12, 543-548.
5. Hu, P., Wu, S., Sun, Y., Yuan, C. C., Kobayashi, R., Myers, M. P. & Hernandez, N. (2002). Mol. Cell. Biol. 22, 8044-8055.
6. Hubbard, S. J. (1998). Biochim. Biophys. Acta, 1382, 191-206.
7. Jasiak, A. J., Armache, K. J., Martens, B., Jansen, R. P. & Cramer, P. (2006). Mol. Cell, 23, 71-81.
8. Kuhn, C. D., Geiger, S. R., Baumli, S., Gartmann, M., Gerber, J., Jennebach, S., Mielke, T., Tschochner, H., Beckmann, R. & Cramer, P. (2007). Cell, 131, 1260-1272.
9. Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press.
10. Meka, H., Daoust, G., Arnvig, K. B., Werner, F., Brick, P. & Onesti, S. (2003). Nucleic Acids Res. 31, 4391-4400.
11. Peyroche, G., Levillain, E., Siaut, M., Callebaut, I., Schultz, P., Sentenac, A., Riva, M. & Carles, C. (2002). Proc. Natl Acad. Sci. USA, 99, 14670-14675.
12. Rost, B., Yachdav, G. & Liu, J. (2004). Nucleic Acids Res. 32, W321-W326.
13. Sadhale, P. P. & Woychik, N. A. (1994). Mol. Cell. Biol. 14, 6164-6170.
14. Shpakovskii, G. V. & Shematorova, E. K. (1999). Bioorg. Khim. 25, 791-796.
15. Siaut, M., Zaros, C., Levivier, E., Ferri, M. L., Court, M., Werner, M., Callebaut, I., Thuriaux, P., Sentenac, A. & Conesa, C. (2003). Mol. Cell. Biol. 23, 195-205.
16. Soding, J., Biegert, A. & Lupas, A. N. (2005). Nucleic Acids Res. 33, W244-W248.
17. Todone, F., Brick, P., Werner, F., Weinzierl, R. O. & Onesti, S. (2001). Mol. Cell, 8, 1137-1143.