Gardnerella vaginalis and Lactobacillus sp in liquid-based cervical samples in healthy and disturbed vaginal flora using cultivation-independent methods

Diagnostic Cytopathology

Volumn 36, Issue 5, 2008, Pages 277-284

  Klomp, Johanna M ^a,b,c   Verbruggen, Banut Sabine M ^a,c   Korporaal, Hans ^a   Boon, Mathilde E ^a   De Jong, Pauline ^a   Kramer, Gerco C ^a   Van Haaften, Maarten ^b   Heintz, A Peter M ^c  

^a LEIDEN CYTOLOGY AND PATHOLOGY LABORATORY   (Netherlands)

^b DEPARTMENT OF SURGERY   (Netherlands)

^c UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

Dysbacteriosis; Gardnerella vaginalis; Lactobacilli; Lactobacillus crispatus; PCR

Indexed keywords

RNA 16S;

ADULT; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL INFECTION; BACTERIUM ADHERENCE; BACTERIUM IDENTIFICATION; FEMALE; GARDNERELLA VAGINALIS; GENE AMPLIFICATION; HUMAN; LACTOBACILLUS; LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS JENSENII; MICROSCOPY; MYCOPLASMA HOMINIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; TECHNIQUE; TECHNOLOGY; UTERINE CERVIX CYTOLOGY; VAGINA FLORA;

ADOLESCENT; ADULT; BACTERIAL ADHESION; BACTERIOLOGICAL TECHNIQUES; CERVIX UTERI; DNA, BACTERIAL; DOUBLE-BLIND METHOD; FEMALE; GARDNERELLA VAGINALIS; HUMANS; LACTOBACILLUS; MIDDLE AGED; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; RNA, RIBOSOMAL, 16S; VAGINA; VAGINAL SMEARS; VAGINOSIS, BACTERIAL;

EID: 43049131097     PISSN: 87551039     EISSN: 10970339     Source Type: Journal    
DOI: 10.1002/dc.20793     Document Type: Article

References (38)

1. Forsum U, Holst E, Larsson PG, Vasquez A, Jakobsson T, Mattsby-Baltzer I. Bacterial vaginosis - A microbiological and immunological enigma. APMIS 2005;113:81-90.
2. Eschenbach DA, Davick PR, Williams BL, et al. Prevalence of hydrogen peroxide-producing lactobacillus species in normal women and women with bacterial vaginosis. J Clin Microbiol 1989;27:251-256.
3. Boris S, Suarez JE, Vazquez F, Barbes C. Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. Infect Immun 1998;66:1985-1989.
4. McLean NW, Rosenstein IJ. Characterisation and selection of a Lactobacillus species to re-colonise the vagina of women with recurrent bacterial vaginosis. J Med Microbiol 2000;49:543-552.
5. Bulk S, van Kemenade FJ, Rozendaal L, Meijer CJLM. The Dutch CISOE-A framework for cytology reporting increases efficacy of screening upon standardisation since 1996. J Clin Pathol 2004;57:388-393.
6. Division of STD Prevention. Bacterial Vaginosis - CDC Fact Sheet. Sexually Transmitted Diseases, Jan 12, 2004. Department of Health and Human services, July 15. 2005. http://www.cdc.gov/std/BV/STDFact-Bacterial-Vaginosis. htm
7. Hillier S, Holmes K. Bacterial vaginosis. Sexual transmitted diseases, 3rd ed. New York: McGraw-Hill; 1999. p 563-586.
8. Hillier SL, Nugent RP, Eschenbach DA, et al. The Vaginal Infections and Prematurity Study Group. Association between bacterial vaginosis and pre-term delivery of low birth weight infant. N Engl J Med 1995;333:1737-1742.
9. Hill GB, Eschenbach DA, Holmes KK. Bacteriology of the vagina. Scanc J Urol Nephrol Suppl 1984;86:23-39.
10. Aroutcheva A, Gariti D, Simon M, et al. Defense factors of vaginal lactobacilli. Am J Obstet Gynecol 2001;185:375-379.
11. Andreu A. Lactobacillus as a probiotic for preventing urogenital infections. Rev Med Microbiol 2004;15:1-6.
12. Mardh PA, Westrom L. Adherence of bacteria to vaginal epithelial cells. Infect Immun 1976;12:661-666.
13. Bibel DJ, Aly R, Lahti L, Shinefield HR, Maibach HI. Microbial adherence to vulvar epithelial cells. J Med Microbiol 1987;23:75-82.
14. Scott TG, Smyth CJ, Keane CT. In vitro adhesiveness and biotype of Gardnerella vaginalis strains in relation to the occurrence of clue cells in vaginal discharges. Genitourin Med 1987;63:47-53.
15. Dedhar L, Karnad J. In vitro adhesiveness of Gardnerella vaginalis strains in relation to the occurrence of clue cells in vaginal discharge. Indian J Med Res 1994;100:59-61.
16. Verbruggen BSM, Boon ME, Boon LM. Dysbacteriosis and squamous (pre)neoplasia of immigrants and Dutch women as established in population-based cervical screening. Diagn Cytopathol 2006;34:377-381.
17. Verbruggen BSM, Boon ME, Schie van MA, Wijsman-Grootendorst R, Kok LP. Dysbacteriosis in silver-stained cervical smears of Dutch-Moroccan Immigrants: HPV infection and preneoplasia. Diagn Cytopathol 2006;34:56-61.
18. Balsdon MJ. Gardnerella vaginalis and its clinical syndrome. Eur J Clin Microbiol 1982;1:288-293.
19. Boon ME, Ouwerkerk-Noordam E, Suurmeijer AJH, Kok LP. Diagnostic parameters in liquid-based cervical cytology using a coagulant suspension fixative. Acta Cytol 2005;49:513-519.
20. Andreu A, Stapleton AE, Fennell CL, Hillier SL, Stamm WE. Hemagglutination, adherence, and surface properties of vaginal Lactobacillus species. J Infect Dis 1995;171:1237-1243.
21. Peeters M, Piot P. Adhesion of Gardnerella vaginalis to vaginal epithelial cells: Variables affecting adhesion and inhibition by metronidazole. Genitourin Med 1985;61:391-395.
22. Sobel JD, Schneider J, Kaye D, Levison ME. Adherence of bacteria to vaginal epithelial cells at various times in the menstrual cycle. Infect Immun 1981;32:194-197.
23. Zariffard MR, Saifuddin M, Sha BE, Spear GT. Detection of bacterial vaginosis related organisms by real-time PCR for Lactobacilli, Gardnerella vaginalis and Mycoplasma hominis. FEMS Immunol Med Microbiol 2002;34:277-281.
24. Boon ME, Kok LP. Formalin cross-linking or optimized alcohol coagulation: That is the question. In: Pandalai SG, editor. Recent research development in human pathology. Trivandrum, Kerala, India: Transworld Research Network; 2005, p 17-38.
25. Giannella C, Zito FA, Colonna F, et al. Comparison of formalin, ethanol, and Histochoice fixation on the PCR amplification from paraffin-embedded breast cancer tissue. Eur J Clin Chem Clin Biochem 1997;35:633-635.
26. Gillespie JW, Best CJ, Bichsel VE, et al. Evaluation of non-formalin tissue fixation for molecular profiling studies. Am J Pathol 2002;160:449-457.
27. Song Y, Kato N, Liu C, Matsumiya Y, Kato H, Watanabe K. Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species-specific primers derived from the 16S-23S rRNA intergenic spacer region and its flanking 23S rRNA. FEMS Microbiol Lett 2000;187:167-173.
28. Baczynska A, Svenstrup HF, Fedder J, Birkelund S, Christiansen G. Development of real-time PCR for detection of Mycoplasma hominis. BMC Microbiol 2004;4:35.
29. Sass MA. Use of a liquid-based, thin-layer Pap test in a community hospital. Impact on cytology performance and productivity. Acta Cytol 2004;48:17-22.
30. Monsonego J, Autillo-Touati A, Bergeron C, Dachez R, et al. Liquid-based cytology for primary cervical cancer screening: a multicentre study. Br J Cancer 2001;84:360-366.
31. Bernstein SJ, Sanchez-Ramos L, Ndubisi B. Liquid-based cervical cytologic smear study and conventional Papanicolaou smears: A metaanalysis of prospective studies comparing cytologic diagnosis and sample adequacy. Am J Obstet Gynecol 2001;185:308-317.
32. Hopwood J, Mallinson H, Hodgson E, Hull L. Liquid based cytology; examination of its potential in a Chlamydia screening programme. Sex Transm Infect 2004;80:371-373.
33. Verhelst R, Verstraelen H, Claeys G, et al. Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis. BMC Microbiol 2004;4:16.
34. Fredricks DN, Fiedler TL, Marrazzo JM. Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med 2005;353:1899-1911.
35. Uchida H, Fujitani K, Kawai Y, et al. A new assay using surface plasmon resonance (SPR) to determine binding of the Lactobacillus acidophilus group to human colonic mucin. Biosci Biotechnol Biochem 2004;68:1004-1010.
36. Strus M, Brzychczy-Wloch M, Kochan P, Heczko P. Hydrogen peroxide produced by Lactobacillus species as a regulatory molecule for vaginal microflora. Med Dosw Mikrobiol 2004;56:67-77 (in Polish).
37. Castagliuolo I, Galeazzi F, Ferrari S, et al. Beneficial effect of autoaggregating Lactobacillus crispatus on experimentally induced colitis in mice. FEMS Immunol Med Microbiol 2005;43:197-204.
38. Demba E, Morison L, van der Loeff MS, et al. Bacterial vaginosis, vaginal flora patterns and vaginal hygiene practices in patients presenting with vaginal discharge syndrome in The Gambia, West Africa. BMC Infect Dis 2005;5:12.