Detection of Pseudo-nitzschia (Bacillariophyceae) species and amnesic shellfish toxins in Scottish coastal waters using oligonucleotide probes and the Jellet Rapid Test™

Harmful Algae

Volumn 7, Issue 4, 2008, Pages 443-458

  Turrell, E ^a   Bresnan, E ^a   Collins, C ^a   Brown, L ^a   Graham, J ^a   Grieve, M ^a  

^a MARINE LABORATORY   (United Kingdom)

Author keywords

Amnesic shellfish poisoning (ASP); Fluorescent in situ hybridisation (FISH); Jellet Rapid Test (JRT); Large sub unit (LSU); Molecular identification; Oligonucleotide probe; Pseudo nitzschia; Transmission electron microscopy (TEM)

Indexed keywords

BACILLARIOPHYCEAE; PSEUDO-NITZSCHIA; PSEUDO-NITZSCHIA AUSTRALIS;

EID: 43049102380     PISSN: 15689883     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.hal.2007.10.001     Document Type: Article

References (36)

1. Andersen P., and Throndsen J. Estimating cell numbers. In: Hallegraeff G.M., Anderson D.M., and Cembella A.D. (Eds). Manual on Harmful Marine Microalgae (2003), Unesco Publishing, Paris, France 99-130
2. Bresnan, E., Fraser, S., Kelly, M.C., 2002. Toxic phytoplankton monitoring in Scotland: 1 January 1999 to 31 December 2000. Fisheries Research Services Report 04/02.
3. Bresnan, E., Fryer, R., Hart, M., Percy, L., 2005. Correlation between algal presence in the water and toxin level in shellfish. Fisheries Research Services Contract Report 04/05, 58pp.
4. Campbell D.A., Kelly M.S., Busman M., Bolch C.J., Wiggins E., Moeller P.D.R., Morton S.L., Hess P., and Shumway S.E. Amnesic shellfish poisoning in the king scallop, Pecten maximus, from the west coast of Scotland. J. Shellfish Res. 20 (2001) 75-84
5. Cusack C., Raine R., and Patching J.W. Occurrence of species from the genus Pseudo-nitzschia Peragallo in Irish waters. Biology and Environment: Proceedings of the Royal Irish Academy, vol. 104B (2004) 55-74
6. Davidson K., and Fehling J. Modelling the influence of silicon and phosphorus limitation on the growth and toxicity of Pseudo-nitzschia seriata. Afr. J. Mar. Sci. 28 (2006) 357-360
7. Doyle J.J., and Doyle J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytoch. Bull. 19 (1987) 11-15
8. Fehling J., Green D.H., and Davidson K. Domoic acid production by Pseudo-nitzschia seriata (Bacillariophyceae) in Scottish waters. J. Phycol. 40 (2004) 622-630
9. Fehling J., Davidson K., Bolch C., and Tett P. Seasonality of Pseudo-nitzschia spp (Bacillariophyceae) in western Scottish waters. Mar. Ecol. Prog. Ser. 323 (2006) 91-105
10. Gallacher S., Howard G., Hess P., MacDonald E., Kelly M.C., Bates L.A., Brown N., MacKenzie M., Gillibrand P., and Turrell W.R. The occurrence of amnesic shellfish poisons in shellfish from Scottish waters. In: Hallegraeff G.M., Blackburn S.I., Bolch C.J., and Lewis R.J. (Eds). Harmful Algal Blooms (2001), IOC of UNESCO, France 30-33
11. Guillard R.R.L. Culture of phytoplankton for feeding marine invertebrates. In: Smith W.L., and Chanley M.H. (Eds). Culture of Marine Invertebrate Animals (1975), Plenum Publishing Corp., New York 83-100
12. Hall T.A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41 (1999) 95-98
13. Howard, F.G., 2002. Monitoring of algal toxins in shellfish in Scotland-1 April 2001 to 31 March 2002. Fisheries Research Services Contract Report 09/02.
14. Howard, F.G., 2003. Monitoring of algal toxins in shellfish in Scotland: 1 April to 31 March 2003. Fisheries Research Services Contract Report 09/03.
15. Jellett J.F., Trainer V., Bates S., and Somerset I. Characteristics and applications of the Jellet Rapid Tests. In: Henshilwood K., Deegan B., McMahon T., Cusack C., Silke J., O'Cinneide M., Lyons D., and Hess P. (Eds). Molluscan Shellfish Safety, Proceedings of the Fifth International Conference on Molluscan Shellfish Safety. Ireland (2006) 6-12
16. Kelly, M., Macdonald, E., 1997. SOAEFD Toxic Algal Monitoring Report: 1 January 1996 to 31 December 1996. Fisheries Research Services Report 02/97.
17. Kelly, M., Fraser, S., 1998. Toxin phytoplankton monitoring in Scotland-1 January to 31 December 1997. Fisheries Research Services Report 06/98.
18. Lundholm N., Daugbjerg N., and Moestrup O. Phylogeny of the Bacillariaceae with emphasis on the genus Pseudo-nitzschia (Bacillariophyceae) based on partial LSU rDNA. Eur. J. Phycol. 37 (2002) 115-134
19. Lundholm N., Moestrup O., Hasle G.R., and Hoef-Emden K. A study of the Pseudo-nitzschia pseudodelicatissima/cuspidata complex (Bacillariophyceae): what is P. pseudodelicatissima?. J. Phycol. 39 (2003) 797-813
20. Lundholm N., Moestrup O., Kotaki Y., Hoef-Emden K., Scholin C., and Miller P. Inter- and intraspecific variation of the Pseudo-nitzschia delicatissima complex (Bacillariophyceae) illustrated by rRNA probes, morphological data and phylogenetic analyses. J. Phycol. 42 (2006) 464-481
21. Miller P.E., and Scholin C.A. Identification of cultured Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU rRNA-targeted fluorescent probes. J. Phycol. 32 (1996) 646-655
22. Miller P.E., and Scholin C.A. Identification and enumeration of cultured and wild Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU rRNA-targeted fluorescent probes and filter-based whole cell hybridization. J. Phycol. 34 (1998) 371-382
23. Miller P.E., and Scholin C.A. On detection of Pseudo-nitzschia (Bacillariophyceae) species using whole cell hybridization: sample fixation and stability. J. Phycol. 36 (2000) 238-250
24. Orsini L., Procaccini G., Sarno D., and Montresor M. Multiple rDNA ITS-types within the diatom Pseudo-nitzschia delicatissima (Bacillariophyceae) and their relative abundance across a spring bloom in the Gulf of Naples. Mar. Ecol. Prog. Ser. 271 (2004) 87-98
25. Orsini L., Sarno D., Procaccini G., Poletti R., Dahlmann J., and Montresor M. Toxic Pseudo-nitzschia multistriata (Bacillariophyceae) from the Gulf of Naples: morphology, toxin analysis and phylogenetic relationships with other Pseudo-nitzschia species. Eur. J. Phycol. 37 (2002) 247-257
26. Percy L., Higman W., Bateman K., Bresnan E., Morris S., and Lewis J. An investigation of the relationship between Pseudonitzschia species and domoic acid in Mytilus sp. in the Fal Estuary UK. 2006. 12th International Conference on Harmful Algae. Copenhagen, Denmark, 4-8 September (2006)
27. Posada D., and Crandall K.A. Selecting models of nucleotide substitution: an application to Human Immunodeficiency Virus 1 (HIV-1). Mol. Biol. Evol. 18 (1998) 897-906
28. Rafuse, C., Silva, M.A., Jellett, J.F., Cembella, A.D., 2001. Rapid monitoring of toxic phytoplankton using the MIST AlertTM for ASP and PSP toxins at aquaculture sites in Atlantic Canada. Aquanet Forum. Dalhousie University.
29. Rhodes L., Scholin C., and Garthwaite I. Pseudo-nitzschia in New Zealand and the role of DNA probes and immunoassays in refining marine biotoxin monitoring programmes. Nat. Toxins 6 (1998) 105-111
30. Scholin C.A., Villac M.C., Buck K.R., Krupp J.M., Powers D.A., Fryxell G.A., and Chavez F.P. Ribosomal DNA sequences discriminate among toxic and non-toxic Pseudonitzschia species. Nat. Toxins 2 (1994) 152-165
31. Scholin C.A., Herzog M., Sogin M., and Anderson D.M. Identification of group and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae) II. Sequence analysis of a fragment of the LSU rRNA gene. J. Phycol. 30 (1994) 999-1011
32. Skov, J., Lundholm, N., Moestrup, O., Larsen, J., 1999. Potentially Toxic Phytoplankton, 4. Pseudo-nitzschia (Diatomophyceae/Bacillariophyceae), ICES Identification Leaflet no. 185, Denmark, 23pp.
33. Smith E.A., Papapanagiotou E.P., Brown N.A., Stobo L.A., Gallacher S., and Shanks A.M. Effect of storage on amnesic shellfish poisoning (ASP) toxins in king scallops (Pecten maximus). Harmful Algae 5 (2006) 9-19
34. Stehr C.M., Connell L.B., Baugh K.A., Bill B.D., Adams N.G., and Trainer V.L. Morphological, toxicological, and genetic differences among Pseudo-nitzschia (Bacillariophyceae) species in inland embayments and outer coastal waters of Washington State USA. J. Phycol. 38 (2002) 55-65
35. Swofford D.L. PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0 (1999), Sinauer Associates, Sunderland, Massachusetts
36. Thompson J.D., Higgins D.G., and Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22 (1994) 4673-4680