Expression, Purification, and Crystallization of Neuro- and Cytoglobin

Methods in Enzymology

Volumn 436, Issue , 2008, Pages 341-357

  Dewilde, Sylvia ^a   Mees, Kirsten ^a   Kiger, Laurent ^b   Lechauve, Christophe ^b   Marden, Michael C ^b   Pesce, Alessandra ^c   Bolognesi, Martino ^d   Moens, Luc ^a  

^a UNIVERSITY OF ANTWERP   (Belgium)

^b University of Genoa ^*

^c UNIVERSITY OF GENOA   (Italy)

^d UNIVERSITY OF MILAN   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOGLOBIN; NEUROGLOBIN; RECOMBINANT PROTEIN; VIRUS VECTOR; ACRYLIC ACID RESIN; GLOBIN; NERVE PROTEIN; SEPHACRYL S 200; SEPHAROSE; SUPEROSE 12;

ANION EXCHANGE CHROMATOGRAPHY; CRYSTAL STRUCTURE; CRYSTALLIZATION; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION SYSTEM; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVIEW; WILD TYPE; ARTICLE; CHEMISTRY; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS;

VERTEBRATA;

ACRYLIC RESINS; CHROMATOGRAPHY, AGAROSE; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; CRYSTALLIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; GLOBINS; HUMANS; MUTATION; NERVE TISSUE PROTEINS; RECOMBINANT PROTEINS; SEPHAROSE;

EID: 42949103602     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(08)36019-4     Document Type: Chapter

References (25)

1. Bashford D., Chothia C., and Lesk A.M. Determinants of a protein fold: Unique features of the globin amino acid sequences. J. Mol. Biol. 196 (1987) 199-216
2. Burmester T., Ebner B., Weich B., and Hankeln T. Cytoglobin: A novel globin type ubiquitously expressed in vertebrate tissues. Mol. Biol. Evol. 19 (2002) 416-421
3. Burmester T., Weich B., Reinhardt S., and Hankeln T. A vertebrate globin expressed in the brain. Nature 407 (2000) 520-523
4. de Sanctis D., Dewilde S., Pesce A., Ascenzi P., Burmester T., Hankeln T., Moens L., and Bolognesi M. New insight into the haemoglobin superfamily: Preliminary crystallographic characterization of human cytoglobin. Acta Crystallogr. D 59 (2003) 1285-1287
5. de Sanctis D., Dewilde S., Pesce A., Moens L., Ascenzi P., Hankeln T., Burmester T., and Bolognesi M. Crystal structure of cytoglobin: The fourth globin type discovered in man displays heme hexa-coordination. J. Mol. Biol. 336 (2004) 917-927
6. Dewilde S., Van Hauwaert M.L., Vinogradov S., Vierstraete A., Vanfleteren J., and Moens L. Protein and gene structure of a chlorocruorin chain of Eudistylia vancouverii. Biochem. Biophys. Res. Commun. 281 (2001) 18-24
7. Dewilde S., Kiger L., Burmester T., Hankeln T., Baudin-Creuza V., Aerts T., Marden M.C., Caubergs R., and Moens L. Biochemical characterization and ligand binding properties of neuroglobin, a novel member of the globin family. J. Biol. Chem. 276 (2001) 38949-38955
8. Fordel E., Geuens E., Dewilde S., Rottiers P., Carmeliet P., Grooten J., and Moens L. Cytoglobin expression is upregulated in all tissues upon hypoxia: An in vitro and in vivo study by quantitative real-time PCR. Biochem. Biophys. Res. Commun. 319 (2004) 342-348
9. Fordel E., Thijs L., Martinet W., Lenjou M., Laufs T., Van Bockstaele D., Moens L., and Dewilde S. Neuroglobin and cytoglobin overexpression protects human SH-SY5Y neuroblastoma cells against oxidative stress-induced cell death. Neurosci. Lett. 410 (2006) 146-151
10. Geuens E., Brouns I., Flamez D., Dewilde S., Timmermans J.P., and Moens L. A globin in the nucleus!. J. Biol. Chem. 278 (2003) 30417-30420
11. Hamdane D., Kiger L., Dewilde S., Green B.N., Pesce A., Uzan J., Burmester T., Hankeln T., Bolognesi M., Moens L., and Marden M.C. The redox state of the cell regulates the ligand binding affinity of human neuroglobin and cytoglobin. J. Biol. Chem. 278 (2003) 51713-51721
12. Khan A.A., Wang Y., Sun Y., Mao X.O., Xie L., Miles E., Graboski J., Chen S., Ellerby L.M., Jin K., and Greenberg D.A. Neuroglobin-overexpressing transgenic mice are resistant to cerebral and myocardial ischemia. Proc. Natl. Acad. Sci. USA 103 (2006) 17944-17948
13. Kiger L., Uzan J., Dewilde S., Burmester T., Hankeln T., Moens L., Hamdane D., Baudin-Creuza V., and Marden M. Neuroglobin ligand binding kinetics. IUBMB Life 56 (2004) 709-719
14. Makino M., Sugimoto H., Sawai H., Kawada N., Yoshizato K., and Shiro Y. High-resolution structure of human cytoglobin: Identification of extra N- and C-termini and a new dimerization mode. Acta Crystallogr. D 62 (2006) 671-677
15. Moens L., Vanfleteren J., Peeters P.Y., Van de K., Kapp O., Czeluzniak J., Goodman M., Blaxter M., and Vinogradov S. Globins in nonvertebrate species: Dispersal by horizontal gene transfer and evolution of the structure-function relationships. Mol. Biol. Evol. 13 (1996) 324-333
16. Pesce A., Bolognesi M., Bocedi A., Ascenzi P., Dewilde S., Moens L., Hankeln T., and Burmester T. Neuroglobin and cytoglobin: Fresh blood for the vertebrate globin family. EMBO Rep. 3 (2002) 1146-1151
17. Pesce A., Dewilde S., Nardini M., Moens L., Ascenzi P., Hankeln T., Burmester T., and Bolognesi M. Human brain neuroglobin structure reveals a distinct mode of controlling oxygen affinity. Structure 11 (2003) 1087-1095
18. Pesce A., Nardini M., Dewilde S., Ascenzi P., Burmester T., Hankeln T., Moens L., and Bolognesi M. Human neuroglobin: Crystals and preliminary X-ray diffraction analysis. Acta Crystallogr. D 58 (2002) 1848-1850
19. Riggs A. Preparation of blood hemoglobins of vertebrates. Methods Enzymol. 76 (1981) 5-29
20. Schmidt M., Giessl A., Laufs T., Hankeln T., Wolfrum U., and Burmester T. How does the eye breathe? Evidence for neuroglobin-mediated oxygen supply in the mammalian retina. J. Biol. Chem. 278 (2003) 1932-1935
21. Sun Y., Jin K., Mao X.O., Zhu Y., and Greenberg D.A. Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic injury. Proc. Natl. Acad. Sci. USA 98 (2001) 15306-15311
22. Sun Y., Jin K., Peel A., Mao X.O., Xie L., and Greenberg D.A. Neuroglobin protects the brain from experimental stroke in vivo. Proc. Natl. Acad. Sci. USA (2003)
23. Trandafir F., Van Doorslaer S., Dewilde S., and Moens L. Temperature dependence of NO binding modes in human neuroglobin. Biochim. Biophys. Acta 1702 (2004) 153-161
24. Trent III J.T., and Hargrove M.S. A ubiquitously expressed human hexacoordinate hemoglobin. J. Biol. Chem. 277 (2002) 19538-19545
25. Van Doorslaer S., Dewilde S., Kiger L., Nistor S.V., Goovaerts E., Marden M.C., and Moens L. Nitric oxide binding properties of neuroglobin: A characterization by EPR and flash photolysis. J. Biol. Chem. 278 (2003) 4919-4925