Assessment of the occurrence of trypanocidal drug resistance in trypanosomes of naturally infected cattle in the Adamaoua region of Cameroon using the standard mouse test and molecular tools

Acta Tropica

Volumn 106, Issue 2, 2008, Pages 115-118

  Mamoudou, A ^a   Delespaux, V ^b   Chepnda, V ^b   Hachimou, Z ^b   Andrikaye, J P ^a   Zoli, A ^a   Geerts, S ^b  

^a UNIVERSITY OF DSCHANG   (Cameroon)

^b INSTITUTE OF TROPICAL MEDICINE   (Belgium)

Author keywords

Cameroon; Diminazene; Drug resistance; Isometamidium; Mouse test; PCR RFLP; Trypanosoma brucei; Trypanosoma congolense

Indexed keywords

ASSESSMENT METHOD; CATTLE; DRUG RESISTANCE; FLAGELLATE; POLYMERASE CHAIN REACTION; RODENT; TESTING METHOD;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CAMEROON; CATTLE; CONTROLLED STUDY; INFECTION CONTROL; MOUSE; NONHUMAN; PARASITE ISOLATION; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; TRYPANOSOMA; TRYPANOSOMA BRUCEI; TRYPANOSOMA CONGOLENSE; TRYPANOSOMIASIS;

ANIMALS; CAMEROON; CATTLE; DIMINAZENE; DNA, PROTOZOAN; DRUG RESISTANCE; MICE; PARASITIC SENSITIVITY TESTS; PHENANTHRIDINES; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; TRYPANOCIDAL AGENTS; TRYPANOSOMA BRUCEI BRUCEI; TRYPANOSOMA CONGOLENSE; TRYPANOSOMIASIS, BOVINE;

ADAMAOUA; AFRICA; CAMEROON; SUB-SAHARAN AFRICA; WEST AFRICA;

BOS; BOVINAE; TRYPANOSOMA BRUCEI; TRYPANOSOMA CONGOLENSE;

EID: 42649122283     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actatropica.2008.02.003     Document Type: Article

References (13)

1. Delespaux V., Ayral F., Geysen D., and Geerts S. PCR-RFLP using Ssu-rDNA amplification: applicability for the diagnosis of mixed infections with different trypanosome species in cattle. Vet. Parasitol. 117 (2003) 185-193
2. Delespaux V., Chitanga S., Geysen D., Goethals A., Van den Bossche P., and Geerts S. SSCP analysis of the P2 purine transporter TcoAT1 gene of Trypanosoma congolense leads to a simple PCR-RFLP test allowing the rapid identification of diminazene resistant stocks. Acta Trop. 100 (2006) 96-102
3. Delespaux V., and de Koning H.P. Drugs and drug resistance in African trypanosomiasis. Drug Resist. Update 10 (2007) 30-50
4. Delespaux V., Geysen D., Majiwa P.A.O., and Geerts S. Identification of a genetic marker for isometamidium chloride resistance in Trypanosoma congolense. Int. J. Parasitol. 35 (2005) 235-243
5. Eisler M.C., Brandt J., Bauer B., Clausen P.H., Delespaux V., Holmes P.H., Ilemobade A., Machila N., Mbwambo H., McDermott J., Mehlitz D., Murilla G., Ndung'u J.M., Peregrine A.S., Sidibe I., Sinyangwe L., and Geerts S. Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle. Vet. Parasitol. 97 (2001) 171-182
6. Geerts, S., Holmes, P.H., 1998. Drug management and parasite resistance in bovine trypanosomiasis in Africa. PAAT Technical Scientific Series 1.
7. Geysen D., Delespaux V., and Geerts S. PCR-RFLP using Ssu-rDNA amplification as an easy method for species-specific diagnosis of Trypanosoma species in cattle. Vet. Parasitol. 110 (2003) 171-180
8. Herbert W.J., and Lumsden W.H.R. Trypanosoma brucei-rapid matching method for estimating host's parasitemia. Exp. Parasitol. 40 (1976) 427-431
9. Holmes P.H., Eisler M.C., and Geerts S. Current chemotherapy of animal trypanosomiasis. In: Maudlin I., Holmes P.H., and Miles M.A. (Eds). The Trypanosomiasis (2004), CABI Publishing, UK
10. Mamoudou A., Zoli A., Tanenbe C., Andrikaye J.P., Bourdanne A., Delespaux V., Clausen P.H., and Geerts S. Evaluation de la résistance aux produits trypanocides sur le plateau de l'Adamaoua au Cameroun en utilisant un test de terrain et le test standardisé sur souris. Rev. Elev. Méd. Vét. Pays Trop. 59 (2006) 11-16
11. Mäser P., Sutterlin C., Kralli A., and Kaminsky R. A nucleoside transporter from Trypanosoma brucei involved in drug resistance. Science 285 (1999) 242-244
12. Murray M., Murray P.K., and Mcintyre W.I.M. Improved parasitological technique for diagnosis of African trypanosomiasis. Trans. R. Soc. Trop. Med. H 71 (1977) 325-326
13. Nerima B., Matovu E., Lubega G.W., and Enyaru J.C. Detection of mutant P2 Adenosine transporter (TbAT1) gene in T. b. gambiense isolates from Northwest Uganda using Allele-specific PCR. Trop. Med. Int. Health 12 (2007) 1361-1368