Controlled microaspiration for high-pressure freezing: A new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography

Journal of Microscopy

Volumn 230, Issue 2, 2008, Pages 278-287

  Triffo, W J ^a,b   Palsdottir, H ^a   McDonald, K L ^c   Lee, J K ^a   Inman, J L ^a   Bissell, M J ^a   Raphael, R M ^b   Auer, M ^a  

^a LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^b Rice University   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Electron tomography; Freeze substitution; High pressure freezing; Mammary organoid; Microdialysis tubing; Outer hair cell

Indexed keywords

ELECTRIC IMPEDANCE TOMOGRAPHY; ELECTRONS; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; TUBING;

BIOLOGICAL SPECIMENS; ELECTRON TOMOGRAPHY; FREEZE SUBSTITUTION; HIGH-PRESSURE FREEZING; MAMMARY ORGANOID; MICRO-DIALYSIS; MICRODIALYSIS TUBING; ORGANOIDS; OUTER HAIR CELL; TEM TOMOGRAPHY;

FREEZING;

ANIMAL CELL; ASPIRATION; CONFERENCE PAPER; CONTROLLED STUDY; ELECTRON MICROSCOPY; EPITHELIUM CELL; FREEZING; GUINEA PIG; MICRODIALYSIS; MICROMANIPULATOR; MOUSE; NONHUMAN; PRIORITY JOURNAL; TISSUE PRESERVATION; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

EID: 42649103668     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2008.01986.x     Document Type: Conference Paper

References (36)

1. Abramoff, M.D., Magelhaes, P.J. & Ram, S.J. (2004) Image processing with ImageJ. Biophoton. Int. 11 (7 36 42.
2. Aggeler, J., Ward, J., Blackie, L.M., Barcellos-Hoff, M.H., Streuli, C.H. & Bissell, M.J. (1991) Cytodifferentiation of mouse mammary epithelial cells cultured on a reconstituted basement membrane reveals striking similarities to development in vivo. J. Cell Sci. 99, 407 417.
3. Barcellos-Hoff, M.H., Aggeler, J., Ram, T.G. & Bissell, M.J. (1989) Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane. Development 105, 223 235.
4. Biel, S.S., Kawaschinski, K., Wittem, K.P., Hintze, U. & Wepf, R. (2003) From tissue to cellular ultrastructure: closing the gap between micro- and nanostructural imaging. J. Microsc. 212 (Pt 1 91 99.
5. Bissell, M.J., Kenny, P.A. & Radisky, D.C. (2005) Microenvironmental regulators of tissue structure and function also regulate tumor induction and progression: the role of extracellular matrix and its degrading enzymes. Cold Spring Harb. Symp. Quant. Biol. 70, 1 14.
6. Brownell, W.E., Bader, C.R., Bertrand, D. & de Ribaupierre, Y. (1985) Evoked mechanical responses of isolated cochlear outer hair cells. Science 227 (4683 194 196.
7. Cardone, G., Grunewald, K. & Steven, A.C. (2005) A resolution criterion for electron tomography based on cross-validation. J. Struct. Biol. 151 (2 117 129.
8. Chertoff, M.E. & Brownell, W.E. (1994) Characterization of cochlear outer hair cell turgor. Am. J. Physiol. 266 (2 Pt 1 C467 C479.
9. Dahl, R. & Staehelin, L.A. (1989) High-pressure freezing for the preservation of biological structure: Theory and practice. J. Electron Microsc. Tech. 13, 165 174.
10. Frey, T.G., Perkins, G.A. & Ellisman, M.H. (2006) Electron tomography of membrane-bound cellular organelles. Annu. Rev. Biophys. Biomol. Struct. 35, 199 224.
11. Frolenkov, G.I. (2006) Regulation of electromotility in the cochlear outer hair cell. J. Physiol. 1, 576 (Pt 1 43 48.
12. Gaietta, G., Deerinck, T.J., Adams, S.R., et al. (2002) Multicolor and electron microscopic imaging of connexin trafficking. Science 296, 503 507.
13. Gilkey, J.C. & Staehelin, L.A. (1986) Advances in ultrarapid freezing for the preservation of cellular ultrastructure. J. Electron Microsc. Techn. 3, 177 210.
14. Grabenbauer, M., Geerts, W.J., Fernadez-Rodriguez, J., Hoenger, A., Koster, A.J. & Nilsson, T. (2005) Correlative microscopy and electron tomography of GFP through photooxidation. Nat. Methods 2, 857 862.
15. Gurr, E. (1971) Synthetic Dyes in Biology, Medicine and Chemistry. Academic Press, London.
16. Hardie, N.A., MacDonald, G. & Rubel, E.W. (2004) A new method for imaging and 3D reconstruction of mammalian cochlea by fluorescent confocal microscopy. Brain Res. 1000, 200 210.
17. He, D.Z., Zheng, J., Kalinec, F., Kakehata, S. & Santos-Sacchi, J. (2006) Tuning in to the amazing outer hair cell: membrane wizardry with a twist and shout. J. Membr. Biol. 209, 119 134.
18. Hohenberg, H., Mannweiler, K. & Müller, M. (1994) High-pressure freezing of cell suspensions in cellulose capillary tubes. J. Microsc. (Oxford) 175, 34 43.
19. Kachar, B., Brownell, W. E., Altschuler, R. & Fex, J. (1986) Electrokinetic shape changes of cochlear outer hair cells. Nature 322, 365 368.
20. Kremer, J.R., Mastronarde, D.N. & McIntosh, J.R. (1996) Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71 76.
21. Kleinman, H.K., McGarvey, M.L., Hassell, J.R., Star, V.L., Cannon, F.B., Laurie, G.W. & Martin, G.R. (1986) Basement membrane complexes with biological activities. Biochemistry 25, 312 318.
22. Krisp, H., von Wichert, G., Seufferlein, T. & Walther, P. (2006) Application of a new high-pressure freezing apparatus. Microsc. Microanal. 12 (Suppl 2). 1112 1113CD
23. Luby-Phelps, K., Ning, G., Fogarty, J. & Besharse, J.C. (2003) Visualization of identified GFP-expressing cells by light and electron microscopy. J. Histoch. Cytoch. 51, 271 274.
24. McDonald, K. (2007) Cryopreparation methods for electron microscopy of selected model systems. Methods Cell. Biol. 79, 23 56.
25. McDonald, K. & Müller-Reichert, T. (2002) Cryomethods for thin section electron microscopy. Guide to Yeast Genetics and Molecular and Cell Biology, Parts B and C (ed. by C. Guthrie and G.R. Fink pp. 96 123. 351, Meth. Enzymol., Academic Press, San Diego.
26. Moor, H. (1987) Theory and practice of high-pressure freezing. Cryo-techniques in Biological Electron Microscopy (ed. by R.A. Steinbrecht and K. Zierold pp. 175 191. Springer-Verlag. Berlin.
27. Müller, M. & Moor, H. (1984) Cryofixation of thick specimens by high-pressure freezing. The Science of Biological Specimen Preparation (ed. by J.P. Revel, T. Barnard and G.H. Haggis pp. 131 138. Proceedings of the 2nd Pfefferkorn Conference, SEM, Inc. AMF O'Hare, IL.
28. Müller-Reichert, T., O'Toole, E.T., Hohenberg, H. & McDonald, K.L. (2003) Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure. J. Microsc. 212, 71 80.
29. Müller-Reichert, T., Srayko, M., Hyman, T., O'Toole, E. & McDonald, K.L. (2007) Correlative light and electron microscopy of early C. elegans embryos in mitosis. Methods Cell. Biol. 79, 101 119.
30. Pelletier, L., O'Toole, E., Schawger, A., Hyman, A.A. & Müller-Reichert, T. (2006) Centriole assembly in Caenorhabditis elegans. Nature 444, 619 623.
31. Pettersen, E.F., Goddard, T.D., Huang, C.C., Couch, G.S., Greenblatt, D.M., Meng, E.C. & Ferrin, T.E. (2004) UCSF chimera - a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605 1612.
32. Sato, T. (1968) A modified method for lead staining of thin sections. J. Electron Microsc. (Tokyo) 17, 158 159.
33. Simian, M., Hirai, Y., Navre, M., Werb, Z., Lochter, A. & Bissell, M.J. (2001) The interplay of matrix metalloproteinases, morphogens and growth factors is necessary for branching of mammary epithelial cells. Development 128, 3117 3131.
34. Spector, A.A., Deo, N., Grosh, K., Ratnanather, J.T. & Raphael, R.M. (2006) Electromechanical models of the outer hair cell composite membrane. J. Membr. Biol. 209, 135 152.
35. Underwood, J.M., Imbalzano, K.M., Weaver, V.M., Fischer, A.H., Imbalzano, A.N. & Nickerson, J.A. (2006) The ultrastructure of MCF-10A acini. J. Cell Physiol. 208, 141 148.
36. Walther, P. & Ziegler, A. (2002) Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208, 3 10.