A PCR for dtxR gene: Application to diagnosis of non-toxigenic and toxigenic Corynebacterium diphtheriae

Molecular and Cellular Probes

Volumn 22, Issue 3, 2008, Pages 189-192

  Pimenta, Fabricia P ^a   Matias, Gisele A M ^a   Pereira, Gabriela A ^a   Camello, Thereza C F ^a   Alves, Gabriela B ^a   Rosa, Ana C P ^a   Hirata Jr , Raphael ^a   Mattos Guaraldi, Ana L ^a  

^a UNIVERSIDADE DO ESTADO DO RIO DE JANEIRO   (Brazil)

Author keywords

C. diphtheriae; dtxR gene; Laboratory diagnosis; PCR

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM COLONY; CORYNEBACTERIUM ACCOLENS; CORYNEBACTERIUM AFERMENTANS; CORYNEBACTERIUM AMYCOLATUM; CORYNEBACTERIUM DIPHTHERIAE; CORYNEBACTERIUM GLUCURONOLYTICUM; CORYNEBACTERIUM JEIKEIUM; CORYNEBACTERIUM MINUTISSIMUM; CORYNEBACTERIUM PROPINQUUM; CORYNEBACTERIUM PSEUDODIPHTHERIAE; CORYNEBACTERIUM STRIATUM; CORYNEBACTERIUM UREALYTICUM; CORYNEBACTERIUM XEROSIS; COST EFFECTIVENESS ANALYSIS; DIAGNOSTIC PROCEDURE; DIAGNOSTIC VALUE; DIPHTHERIA; DTXR GENE; GENE IDENTIFICATION; GORDONIA; GRAM NEGATIVE BACTERIUM; HEALTH CARE COST; HOSPITAL LABORATORY; LABORATORY DIAGNOSIS; LISTERIA; NOCARDIA; NONHUMAN; OERSKOVIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RHODOCOCCUS EQUI; SCREENING TEST; SPECIES DIFFERENCE;

BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; CORYNEBACTERIUM DIPHTHERIAE; DIPHTHERIA; DIPHTHERIA TOXIN; DNA-BINDING PROTEINS; ENDOCARDITIS; GENES, BACTERIAL; HUMANS; POLYMERASE CHAIN REACTION;

CORYNEBACTERIUM; CORYNEBACTERIUM DIPHTHERIAE; POSIBACTERIA;

EID: 42649097219     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2008.01.001     Document Type: Article

References (20)

1. Bonnet J.M., and Begg N.T. Control of diphtheria: guidance for consultants in communicable disease control. Commun Dis Public Health 2 (1999) 242-249
2. Reacher M., Ramsay M., White J., De Zoysa A., Efstratiou A., Mann G., et al. Nontoxigenic Corynebacterium diphtheriae: an emerging pathogen in England and Wales?. Emerging Infect Dis 6 (2000) 640-645
3. De Zoysa A., Efstratiou A., and Hawkey P.M. Molecular characterization of diphtheria toxin repressor (DtxR) genes present in nontoxigenic Corynebacterium diphtheriae strains isolated in the United Kingdom. J Clin Microbiol 43 (2005) 223-228
4. Pennie R.A., Malik A.S., and Wilcox L. Misidentification of toxigenic Corynebacterium diphtheriae as a Corynebacterium species with low virulence in a child with endocarditis. J Clin Microbiol 34 (1996) 1275-1276
5. Klena J.D., Parker C.T., Knibb K., Ibbitt J.C., Devane P.M., Horn S.T., et al. Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA. J Clin Microbiol 42 (2004) 5549-5557
6. Nakao H., Pruckler J.M., Mazurova I.K., Narvskaia O.V., Glushkevich T., Marijevski V.F., et al. Heterogeneity of diphtheria toxin gene, tox, and its regulatory element, dtxR, in Corynebacterium diphtheriae strains causing epidemic diphtheria in Russia and the Ukraine. J Clin Microbiol 34 (1996) 1711-1716
7. Nakao H., Mazurova I.K., Glushkevich T., and Popovic T. Analysis of heterogeneity of Corynebacterium diphtheriae toxin gene, tox, and its regulatory element, dtxR, by direct sequencing. Res Microbiol 148 (1997) 45-54
8. Oram D.M., Avdalovic A., and Holmes R.K. Analysis of genes that encode DtxR-like transcriptional regulators in pathogenic and saprophytic corynebacterial species. Infect Immun 72 (2004) 1885-1895
9. Oguiza J.A., Tao X., Marcos A.T., Martin J.F., and Murphy J.R. Molecular cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR homolog from Brevibacterium lactofermentum. J Bacteriol 177 (1995) 465-467
10. Schmitt M.P., Predich M., Doukhan L., Smith I., and Holmes R.K. Characterization of an iron-dependent regulatory protein (IdeR) of Mycobacterium tuberculosis as a functional homolog of the diphtheria toxin repressor (DtxR) from Corynebacterium diphtheriae. Infect Immun 63 (1995) 4284-4289
11. Boland C.A., and Meijer W.G. The iron dependent regulatory protein IdeR (DtxR) of Rhodococcus equi. FEMS Microbiol Lett 191 (2000) 1-5
12. Tang Y.W., Von Graevenitz A., Waddington M.G., Hopkins M.K., Smith D.H., Li H., et al. Identification of coryneform bacterial isolates by ribosomal DNA sequence analysis. J Clin Microbiol 38 (2000) 1676-1678
13. Hou X.G., Kawamura Y., Sultana F., Hirose K., Miyake M., Otsuka Y., et al. Genetic identification of members of the genus Corynebacterium at genus and species levels with 16S rDNA-targeted probes. Microbiol Immunol 41 (1997) 453-460
14. Efstratiou A., and George R.C. Laboratory guidelines for the diagnosis of infections caused by Corynebacterium diphtheriae and C. ulcerans. World Health Organization. Commun Dis Public Health 2 (1999) 250-257
15. Camello T.C.F., Mattos-Guaraldi A.L., Formiga L.C.D., and Marques E.A. Nondiphtherial Corynebacterium species isolated from various clinical sources of patients from a teaching hospital, Rio de Janeiro, Brazil. Braz J Microbiol 4 (2003) 39-44
16. Funke G, Bernard AK, Coryneform Gram-positive rods. In: Manual of clinical microbiology, vol. 1. Washington; 2003. pp. 472-501.
17. Soto A., Zapardiel J., and Soriano F. Evaluation of API Coryne system for identifying coryneform bacteria. J Clin Pathol 47 (1994) 756-759
18. Efstratiou A., Engler K.H., Dawes C.S., and Sesardic D. Comparison of phenotypic and genotypic methods for detection of diphtheria toxin among isolates of pathogenic corynebacteria. J Clin Microbiol 36 (1998) 3173-3177
19. Pallen M.J., Hay A.J., Puckey L.H., and Efstratiou A. Polymerase chain reaction for screening clinical isolates of corynebacteria for the production of diphtheria toxin. J Clin Pathol 47 (1994) 353-356
20. Efstratiou A., and George R.C. Screening tests for the presumptive identification of Corynebacterium diphtheriae in a diagnostic laboratory. J Clin Microbiol 34 (1996) 3251