Expression, purification and characterization of anti-BAFF antibody secreted from the yeast Pichia pastoris

Biotechnology Letters

Volumn 30, Issue 6, 2008, Pages 1075-1080

  Ren, Fang ^a   Li, Bao Cun ^a   Zhang, Na Na ^a   Cao, Meng ^a   Dan, Wen Bing ^a   Zhang, Shuang Quan ^a  

^a NANJING NORMAL UNIVERSITY   (China)

Author keywords

B cell activating factor; Binding activity; Heterologous expression; Pichia pastoris; scFv Fc

Indexed keywords

ANTIBODIES; BIOASSAY; CELL CULTURE; CHARACTERIZATION; GENE EXPRESSION; PURIFICATION;

B-CELL ACTIVATING FACTORS; BINDING ACTIVITY; EUKARYOTIC EXPRESSION; HETEROLOGOUS EXPRESSION; PICHIA PASTORIS;

YEAST;

ANTIBODY; B CELL ACTIVATING FACTOR; IMMUNOGLOBULIN FC FRAGMENT;

ANTIBODY SPECIFICITY; ARTICLE; DRUG ANTAGONISM; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GENE VECTOR; GENETIC TRANSFORMATION; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PICHIA; PLASMID;

ANTIBODIES; ANTIBODY SPECIFICITY; B-CELL ACTIVATING FACTOR; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GENETIC VECTORS; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; PICHIA; PLASMIDS; TRANSFORMATION, GENETIC;

EUKARYOTA; PICHIA PASTORIS;

EID: 42549167603     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9657-x     Document Type: Article

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