A modified DNA isolation protocol for obtaining pure RT-PCR grade RNA

Biotechnology Letters

Volumn 30, Issue 6, 2008, Pages 1041-1044

  Klassen, Roland ^a   Fricke, Julia ^a   Pfeiffer, Annika ^a   Meinhardt, Friedhelm ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

RNA isolation; RT PCR; Yeast

Indexed keywords

BIOASSAY; CHROMOSOMES; MOLECULAR WEIGHT; POLYPEPTIDES; VERIFICATION; YEAST;

COST EFFICIENCY; RNA ISOLATION; TRNA GENES;

DNA SEQUENCES;

DEOXYRIBONUCLEASE I; FUNGAL DNA; FUNGAL RNA; MESSENGER RNA; RIBOSOME RNA; RNA 5.8S; RNA 5S; TRANSFER RNA;

ARTICLE; ISOLATION AND PURIFICATION; METABOLISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

DEOXYRIBONUCLEASE I; DNA, FUNGAL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, FUNGAL; RNA, MESSENGER; RNA, RIBOSOMAL; RNA, RIBOSOMAL, 5.8S; RNA, RIBOSOMAL, 5S; RNA, TRANSFER; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 42549094657     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9648-y     Document Type: Article

References (12)

1. Amberg DC, Burke DJ, Strathern JN (2005). Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
2. Arthington BA, Bennett LG, Skatrud PL, Guynn CJ, Barbuch RJ, Ulbright CE, Bard M (1991) Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase. Gene 102:39-44
3. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1994) Current protocols in molecular biology. Wiley, New York
4. van Dijken JP, Bauer J, Brambilla L, Duboc P, Francois JM, Gancedo C, Giuseppin ML, Heijnen JJ, Hoare M, Lange HC, Madden EA, Niederberger P, Nielsen J, Parrou JL, Petit T, Porro D, Reuss M, van Riel N, Rizzi M, Steensma HY, Verrips CT, Vindelov J, Pronk JT (2000) An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains. Enzyme Microb Technol 26:706-714
5. Gasch AP (2003) The environmental stress response: a common yeast response to diverse environmental stresses. In: Hohmann S, Mager PWH (eds) Yeast stress responses, topics in current genetics, vol 1. Springer-Verlag, Berlin, Heidelberg, pp 11-70
6. Kawasaki ES, Clark SS, Coyne MY, Smith SD, Champlin R, Witte ON, McCormick FP (1988) Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc Natl Acad Sci USA 85:5698-5702
7. Mendes-Ferreira A, del Olmo M, García-Martínez J, Jiménez-Martí E, Mendes-Faia A, Pérez-Ortín JE, Leão C (2007) Transcriptional response of Saccharomyces cerevisiae to different nitrogen concentrations during alcoholic fermentation. Appl Environ Microbiol 73:3049-3060
8. Rossignol T, Dulau L, Julien A, Blondin B (2003) Genome-wide monitoring of wine yeast gene expression during alcoholic fermentation. Yeast 20:1369-1385
9. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350-1354
10. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487-491
11. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
12. Weber E, Rodriguez C, Chevallier MR, Jund R (1990) The purine-cytosine permease gene of Saccharomyces cerevisiae: primary structure and deduced protein sequence of the FCY2 gene product. Mol Microbiol 4:585-596