Analytical and densitometric evaluation of visualizing reagents of selected aliphatic compounds on thin layer

Journal of Liquid Chromatography and Related Technologies

Volumn 31, Issue 10, 2008, Pages 1492-1510

  Pyka, A ^a   Klimczok, W ^a  

^a MEDICAL UNIVERSITY OF SILESIA   (Poland)

Author keywords

Broadening index; Densitometric visualizing index; Densitometry; Detectability; Detection index; Dyes; Methyl stearate; Modified contrast index; New visualizing reagents; NP TLC; Stearic acid; Stearyl alcohol

Indexed keywords

BROADENING INDEX; DENSITOMETRIC VISUALIZING INDEX; DETECTABILITY; DETECTION INDEX; METHYL STEARATE; MODIFIED CONTRAST INDEX; NEW VISUALIZING REAGENTS; STEARYL ALCOHOLS;

DENSITOMETERS; PARAMETER ESTIMATION; SILICA GEL; STEARIC ACID;

DYES;

ALIPHATIC COMPOUND; CRYSTAL VIOLET; JANUS BLUE; MALACHITE GREEN; METHYL GREEN; METHYLENE BLUE; METHYLENE VIOLET; OCTADECANOL; REAGENT; RHODAMINE B; SILICA GEL; STEARIC ACID; STEARIC ACID METHYL ESTER; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; COMPARATIVE STUDY; DENSITOMETRY; QUANTITATIVE ANALYSIS;

GENTIANA;

EID: 42449084178     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826070802039556     Document Type: Article

References (15)

1. Niestroj, A.; Stefaniak, M.; Sliwiok, J. Application of Rhodamine B in densitometric determination of long chain fatty acids in thin layer chromatography. Chemistry and Biochemistry in the Agricultural Production, Environmental Protection, Human and Animal Health; Gorecki, H., Dobrzanski, Z. and Kafarski, P., Eds.; Prague-Brussels: Czech-Pol-Trade, 2006; 1-4.
2. Fried, B. Lipids In Handbook of Thin-Layer Chromatography: Third Edition, Revised and Expanded; Marcel Dekker, Inc.: New York, 2003; 635-670.
3. Jork, H.; Funk, W.; Fischer, W.; Wimmer, H. Dünnschicht- Chromatographie, Reagenzien und Nachweismethoden, Physicalische und Chemische Nachweismethoden: Grundlagen, Reagenzien I; VCH: Weinheim: Germany, 1989.
4. Jork, H.; Funk, W.; Fischer, W.; Wimmer, H. Thin-Layer Chromatography: Reagents and Detection Methods, Vol 1b, Physical and Chemical Detection Methods: Activation Reactions, Reagents Sequences, Reagents II; VCH: Weinheim: Germany, 1994.
5. Gasparic, J.; Churacek, J. Laboratory Handbook of Paper and Thin-Layer Chromatography; John Wiley & Sons Inc.: New York, 1978.
6. Sliwiok, J. The Application of fuchsine dyes in the detection of higher fatty acids by thin-layer chromatography. Microchem. J. 1968, 13 (1), 108-110.
7. Kishimoto, K.; Urade, R.; Ogawa, T.; Moriyama, T. Nondestructive quantification of neutral lipids by thin-layer chromatography and laser-fluorescent scanning: suitable methods for "Lipidome" analysis. Biochem. Biophys. Res. Comm. 2001, 281, 657-662.
8. Pyka, A.; Sliwiok, J. Application of thymol blue and bromothymol blue to the detection of the alcohols in TLC. J. Planar Chromatogr. Mod. TLC 1992, 5, 282-283.
9. Wardas, W.; Pyka, A. New visualizing agents for selected fatty derivatives in thin layer chromatography. J. Planar Chromatogr. Mod. TLC 1993, 6, 320-322.
10. Wardas, W.; Pyka, A. Visualizing agents for higher fatty acids in TLC. In Dunnschicht-Chromatographie; Kaiser, R.E., Gunther, W., Gunz, H. and Wulff, G., Eds.; Rudolf Stehle GmbH & Co.: Dusseldorf, KG, 1996, 196-201.
11. Wardas, W.; Pyka, A. Visualizing agents for esters of higher fatty acids in TLC. J. Planar Chromatogr. Mod. TLC 2001, 14, 8-15.
12. Pyka, A. The application of densitometry to evaluation of visualizing effects of salicylanilide using brilliant green. J. Liq. Chromatogr. & Rel. Technol. 2008, 31, in press.
13. Gregorowicz, Z.; Sliwiok, J. Indexes for estimation of developing reagents in thinlayer chromatography. Microchem. J. 1970, 15 (1), 60-63.
14. Pyka, A.; Klimczok, W.; Gurak, D. Evaluation of visualizing reagents for estradiol on thin layer by densitometric method. J. Liq. Chromatogr. & Rel. Technol. 2008, 31, 555-566.
15. Klimczok, W.; Pyka, A.; Gurak, D. Application of densitometry and spectrodensitometry for the evaluation of the new visualizing reagents for selected drugsp61 The XXXI Symposium "Chromatographic Methods of Investigating the Organic Compounds", Katowice-Szczyrk, June 4th-6th, 2007, P-14, p. 16.