메뉴 건너뛰기




Volumn 74, Issue 8, 2008, Pages 2505-2510

Improved methodology for identification of protists and microalgae from plankton samples preserved in Lugol's iodine solution: Combining microscopic analysis with single-cell PCR

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVITY; GENE ENCODING; RNA;

EID: 42349111816     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01803-07     Document Type: Article
Times cited : (86)

References (33)
  • 1
    • 33644782643 scopus 로고    scopus 로고
    • Adl, S. M., A. G. B. Simpson, M. A. Farmer, R. A. Andersen, O. R. Anderson, J. R. Barta, S. S. Bowser, G. Brugerolle, R. A. Fensome, S. Frederico, T. Y. James, S. Karpov, P. Kugrens, J. Krug, C. E. Lane, L. A. Lewis, J. Lodge, D. H. Lynn, D. G. Mann, R. M. McCourt, L. Mendoza, Ø. Moestrup, S. E. Mozley-Standridge, T. A. Nerad, C. A. Shearer, A. V. Smirnov, F. W. Spiegel, and M. F. J. R. Taylor. 2005. The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. J. Eukaryot. Microbiol. 52:399-451.
    • Adl, S. M., A. G. B. Simpson, M. A. Farmer, R. A. Andersen, O. R. Anderson, J. R. Barta, S. S. Bowser, G. Brugerolle, R. A. Fensome, S. Frederico, T. Y. James, S. Karpov, P. Kugrens, J. Krug, C. E. Lane, L. A. Lewis, J. Lodge, D. H. Lynn, D. G. Mann, R. M. McCourt, L. Mendoza, Ø. Moestrup, S. E. Mozley-Standridge, T. A. Nerad, C. A. Shearer, A. V. Smirnov, F. W. Spiegel, and M. F. J. R. Taylor. 2005. The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. J. Eukaryot. Microbiol. 52:399-451.
  • 2
    • 22144433742 scopus 로고    scopus 로고
    • Development of new procedures for the isolation of phytoplankton DNA from fixed samples
    • Bertozzini, E., A. Penna, E. Pierboni, I. Bruce, and M. Magnani. 2005. Development of new procedures for the isolation of phytoplankton DNA from fixed samples. J. Appl. Phycol. 17:223-229.
    • (2005) J. Appl. Phycol , vol.17 , pp. 223-229
    • Bertozzini, E.1    Penna, A.2    Pierboni, E.3    Bruce, I.4    Magnani, M.5
  • 3
    • 17844394957 scopus 로고    scopus 로고
    • High diversity of the "Spumella-like" flagellates: An investigation based on the SSU rRNA gene sequences of isolates from habitats located in six different geographic regions
    • Boenigk, J., K. Pfandl, P. Stadler, and A. Chatzinotas. 2005. High diversity of the "Spumella-like" flagellates: an investigation based on the SSU rRNA gene sequences of isolates from habitats located in six different geographic regions. Environ. Microbiol. 7:685-697.
    • (2005) Environ. Microbiol , vol.7 , pp. 685-697
    • Boenigk, J.1    Pfandl, K.2    Stadler, P.3    Chatzinotas, A.4
  • 4
    • 15044365131 scopus 로고    scopus 로고
    • DNA and RNA obtained from Bouin's fixed tissues
    • Bonin, S., F. Petrera, J. Rosai, and G. Stanta. 2005. DNA and RNA obtained from Bouin's fixed tissues. J. Clin. Pathol. 58:313-316.
    • (2005) J. Clin. Pathol , vol.58 , pp. 313-316
    • Bonin, S.1    Petrera, F.2    Rosai, J.3    Stanta, G.4
  • 5
    • 0033760612 scopus 로고    scopus 로고
    • Development of real-time PCR assays for rapid detection of Pfisteria piscida and related dinoflagellates
    • Bowers, H. A., T. Tengs, H. B. Glasgow, J. M. Burkholder, P. A. Rublee, and D. W. Oldach. 2000. Development of real-time PCR assays for rapid detection of Pfisteria piscida and related dinoflagellates. Appl. Environ. Microbiol. 66:4641-4648.
    • (2000) Appl. Environ. Microbiol , vol.66 , pp. 4641-4648
    • Bowers, H.A.1    Tengs, T.2    Glasgow, H.B.3    Burkholder, J.M.4    Rublee, P.A.5    Oldach, D.W.6
  • 6
    • 0036060997 scopus 로고    scopus 로고
    • Rapid identification of marine algae (Raphidophyceae) using three-primer PCR amplification of nuclear internal transcribed spacer (ITS) regions from fresh and archived material
    • Connell, L. 2002. Rapid identification of marine algae (Raphidophyceae) using three-primer PCR amplification of nuclear internal transcribed spacer (ITS) regions from fresh and archived material. Phycologia 41:15-21.
    • (2002) Phycologia , vol.41 , pp. 15-21
    • Connell, L.1
  • 7
    • 42349091667 scopus 로고    scopus 로고
    • New species of Uroglena and Ochromonas (Chromulinales, Chrysophyceae) from Estonia
    • Cronberg, G., and R. Laugaste. 2005. New species of Uroglena and Ochromonas (Chromulinales, Chrysophyceae) from Estonia. Nova Hedwigia Beih. 128:43-63.
    • (2005) Nova Hedwigia Beih , vol.128 , pp. 43-63
    • Cronberg, G.1    Laugaste, R.2
  • 8
    • 0032486094 scopus 로고    scopus 로고
    • DNA damage caused by common cytological fixatives
    • Douglas, M. P., and S. O. Rogers. 1998. DNA damage caused by common cytological fixatives. Mutat. Res. 401:77-88.
    • (1998) Mutat. Res , vol.401 , pp. 77-88
    • Douglas, M.P.1    Rogers, S.O.2
  • 9
    • 0029346991 scopus 로고
    • Use of the PCR and fluorescent-probes to recover SSU ribosomal-RNA gene-sequences from single cells of the ciliate protozoan Spathidium
    • Dyal, P. L., S. Hope, D. M. Roberts, and T. M. Embley. 1995. Use of the PCR and fluorescent-probes to recover SSU ribosomal-RNA gene-sequences from single cells of the ciliate protozoan Spathidium. Mol. Ecol. 4:499-503.
    • (1995) Mol. Ecol , vol.4 , pp. 499-503
    • Dyal, P.L.1    Hope, S.2    Roberts, D.M.3    Embley, T.M.4
  • 10
    • 0037394620 scopus 로고    scopus 로고
    • Genetic variability and molecular phylogeny of Dinophysis species (Dinophyceae) from Norwegian waters inferred from single cell analyses of rDNA
    • Edvardsen, B., K. Shalchian-Tabrizi, K. S. Jakobsen, L. K. Medlin, E. Dahl, S. Brubak, and E. Paasche. 2003. Genetic variability and molecular phylogeny of Dinophysis species (Dinophyceae) from Norwegian waters inferred from single cell analyses of rDNA. J. Phycol. 39:295-408.
    • (2003) J. Phycol , vol.39 , pp. 295-408
    • Edvardsen, B.1    Shalchian-Tabrizi, K.2    Jakobsen, K.S.3    Medlin, L.K.4    Dahl, E.5    Brubak, S.6    Paasche, E.7
  • 11
    • 2342541675 scopus 로고    scopus 로고
    • Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a dinoflagellate)
    • Galluzzi, L., A. Penna, E. Bertozzini, M. Vila, E. Garcés, and M. Magnani. 2004. Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a dinoflagellate). Appl. Environ. Microbiol. 70:1199-1206.
    • (2004) Appl. Environ. Microbiol , vol.70 , pp. 1199-1206
    • Galluzzi, L.1    Penna, A.2    Bertozzini, E.3    Vila, M.4    Garcés, E.5    Magnani, M.6
  • 12
    • 0001412719 scopus 로고    scopus 로고
    • Sampling, preservation, enumeration and biomass of marine protozooplankton
    • R. P. Harris, P. H. Weihe, J. Lenz, H. R. Skjoldal, and M. Huntley ed, Academic Press, London, United Kingdom
    • Gifford, D. J., and D. A. Caron. 2000. Sampling, preservation, enumeration and biomass of marine protozooplankton, p. 193-221. In R. P. Harris, P. H. Weihe, J. Lenz, H. R. Skjoldal, and M. Huntley (ed.), ICES zooplankton methodology manual. Academic Press, London, United Kingdom.
    • (2000) ICES zooplankton methodology manual , pp. 193-221
    • Gifford, D.J.1    Caron, D.A.2
  • 13
    • 0242628758 scopus 로고    scopus 로고
    • PCR amplification of microalgal DNA for sequencing and species identification: Studies on fixatives and algal growth stages
    • Godhe, A., D. M. Anderson, and A.-S. Rehnstam-Holm. 2002. PCR amplification of microalgal DNA for sequencing and species identification: studies on fixatives and algal growth stages. Harmful Algae 1:375-382.
    • (2002) Harmful Algae , vol.1 , pp. 375-382
    • Godhe, A.1    Anderson, D.M.2    Rehnstam-Holm, A.-S.3
  • 14
    • 0036745575 scopus 로고    scopus 로고
    • Genetic diversity and molecular detection of three toxic dinoflagellate genera (Alexandrium, Dinophysis, and Karenia) from French coasts
    • Guillou, L., E. Nezan, V. Cueff, E. E. L. Denn, M. A. Cambon-Bonavita, P. Gentien, and G. Barbier. 2002. Genetic diversity and molecular detection of three toxic dinoflagellate genera (Alexandrium, Dinophysis, and Karenia) from French coasts. Protist 153:223-238.
    • (2002) Protist , vol.153 , pp. 223-238
    • Guillou, L.1    Nezan, E.2    Cueff, V.3    Denn, E.E.L.4    Cambon-Bonavita, M.A.5    Gentien, P.6    Barbier, G.7
  • 15
    • 0345269205 scopus 로고    scopus 로고
    • Isolation of novel ultramicrobacteria classified as Actinobacteria from five freshwater habitats in Europe and Asia
    • Hahn, M. W., H. Lünsdorf, Q. L. Wu, M. Schauer, M. G. Höfle, J. Boenigk, and P. Stadler. 2003. Isolation of novel ultramicrobacteria classified as Actinobacteria from five freshwater habitats in Europe and Asia. Appl. Environ. Microbiol. 69:1442-1451.
    • (2003) Appl. Environ. Microbiol , vol.69 , pp. 1442-1451
    • Hahn, M.W.1    Lünsdorf, H.2    Wu, Q.L.3    Schauer, M.4    Höfle, M.G.5    Boenigk, J.6    Stadler, P.7
  • 16
    • 19944376518 scopus 로고    scopus 로고
    • Integrated method for single-cell DNA extraction, PCR amplification, and sequencing of the ribosomal DNA from harmful dinoflagellates Cochlodinium polykrikoides and Alexandrium catenella
    • Ki, J. S., G. Y. Jang, and M. S. Han. 2004. Integrated method for single-cell DNA extraction, PCR amplification, and sequencing of the ribosomal DNA from harmful dinoflagellates Cochlodinium polykrikoides and Alexandrium catenella. Mar. Biotechnol. 6:587-593.
    • (2004) Mar. Biotechnol , vol.6 , pp. 587-593
    • Ki, J.S.1    Jang, G.Y.2    Han, M.S.3
  • 18
    • 0035186948 scopus 로고    scopus 로고
    • Preparation of DNA suitable for PCR amplification from fresh and fixed single dinoflagellate cells
    • Marin, I., A. Aguilera, B. Reguera, and J. Abad. 2001. Preparation of DNA suitable for PCR amplification from fresh and fixed single dinoflagellate cells. BioTechniques 30:88-93.
    • (2001) BioTechniques , vol.30 , pp. 88-93
    • Marin, I.1    Aguilera, A.2    Reguera, B.3    Abad, J.4
  • 20
    • 26844449343 scopus 로고    scopus 로고
    • Effects of fixatives on ciliates as related to cell size
    • Modigh, M., and S. Castaldo. 2005. Effects of fixatives on ciliates as related to cell size. J. Plankton Res. 27:845-849.
    • (2005) J. Plankton Res , vol.27 , pp. 845-849
    • Modigh, M.1    Castaldo, S.2
  • 21
    • 0021239665 scopus 로고
    • Direct counting of bacteria preserved with Lugol iodine solution
    • Pomroy, A. J. 1984. Direct counting of bacteria preserved with Lugol iodine solution. Appl. Environ. Microbiol. 47:1191-1192.
    • (1984) Appl. Environ. Microbiol , vol.47 , pp. 1191-1192
    • Pomroy, A.J.1
  • 22
    • 17444383839 scopus 로고    scopus 로고
    • Molecular identity of strains of heterotrophic flagellates isolated from surface waters and deep-sea sediments of the South Atlantic based on SSU rDNA
    • Scheckenbach, F., C. Wylezich, M. Weitere, K. Hausmann, and H. Arndt. 2005. Molecular identity of strains of heterotrophic flagellates isolated from surface waters and deep-sea sediments of the South Atlantic based on SSU rDNA. Aquat. Microb. Ecol. 38:239-247.
    • (2005) Aquat. Microb. Ecol , vol.38 , pp. 239-247
    • Scheckenbach, F.1    Wylezich, C.2    Weitere, M.3    Hausmann, K.4    Arndt, H.5
  • 23
    • 1842527149 scopus 로고    scopus 로고
    • Temporal patterns in population genetic diversity of Prorocentrum micans (Dinophyceae)
    • Shankle, A. M., X. Mayali, and J. S. Franks. 2004. Temporal patterns in population genetic diversity of Prorocentrum micans (Dinophyceae). J. Phycol. 40:239-247.
    • (2004) J. Phycol , vol.40 , pp. 239-247
    • Shankle, A.M.1    Mayali, X.2    Franks, J.S.3
  • 24
    • 0001401636 scopus 로고
    • Preservation and storage of samples for enumeration of heterotrophic protists
    • P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole ed, Lewis Publishers, London, United Kingdom
    • Sherr, E. B., and B. F. Sherr. 1993. Preservation and storage of samples for enumeration of heterotrophic protists, p. 207-212. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole (ed.), Handbook of methods in aquatic microbial ecology. Lewis Publishers, London, United Kingdom.
    • (1993) Handbook of methods in aquatic microbial ecology , pp. 207-212
    • Sherr, E.B.1    Sherr, B.F.2
  • 25
    • 26244431747 scopus 로고    scopus 로고
    • The extent of protist diversity: Insights from molecular ecology of freshwater eukaryotes
    • Slapeta, J., D. Moreira, and P. Lopez-Garcia. 2005. The extent of protist diversity: insights from molecular ecology of freshwater eukaryotes. Proc. Biol. Sci. 272:2073-2081.
    • (2005) Proc. Biol. Sci , vol.272 , pp. 2073-2081
    • Slapeta, J.1    Moreira, D.2    Lopez-Garcia, P.3
  • 26
    • 0036898005 scopus 로고    scopus 로고
    • Effect of fixatives and tissue processing on the content and integrity of nucleic acids
    • Srinivasan, M., D. Sedmak, and S. Jewell. 2002. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am. J. Pathol. 161:1961-1971.
    • (2002) Am. J. Pathol , vol.161 , pp. 1961-1971
    • Srinivasan, M.1    Sedmak, D.2    Jewell, S.3
  • 27
    • 0038221066 scopus 로고    scopus 로고
    • Novel eukaryotic lineages inferred from small-subunit rRNA analyses of oxygen-depleted marine environments
    • Stoeck, T., and S. Epstein. 2003. Novel eukaryotic lineages inferred from small-subunit rRNA analyses of oxygen-depleted marine environments. Appl. Environ. Microbiol. 69:2657-2663.
    • (2003) Appl. Environ. Microbiol , vol.69 , pp. 2657-2663
    • Stoeck, T.1    Epstein, S.2
  • 28
    • 32344437178 scopus 로고    scopus 로고
    • A multiple PCR-primer approach to access the microeukaryotic diversity in environmental samples
    • Stoeck, T., B. Hayward, G. T. Taylor, R. Varela, and S. S. Epstein. 2006. A multiple PCR-primer approach to access the microeukaryotic diversity in environmental samples. Protist 157:31-43.
    • (2006) Protist , vol.157 , pp. 31-43
    • Stoeck, T.1    Hayward, B.2    Taylor, G.T.3    Varela, R.4    Epstein, S.S.5
  • 29
    • 33645227135 scopus 로고    scopus 로고
    • Acquiring scanning electron microscopical, light microscopical and multiple gene sequence data from a single dinoflagellate cell
    • Takano, V., and T. Horiguchi. 2005. Acquiring scanning electron microscopical, light microscopical and multiple gene sequence data from a single dinoflagellate cell. J. Phycol. 42:251-256.
    • (2005) J. Phycol , vol.42 , pp. 251-256
    • Takano, V.1    Horiguchi, T.2
  • 30
    • 0035076375 scopus 로고    scopus 로고
    • Genetic polymorphism in Gymnodinium galatheanum chloroplast DNA sequences and the development of a molecular detection assay
    • Tengs, T., H. A. Bowers, A. P. Ziman, D. K. Stoecker, and D. W. Oldach. 2001. Genetic polymorphism in Gymnodinium galatheanum chloroplast DNA sequences and the development of a molecular detection assay. Mol. Ecol. 10:515-523.
    • (2001) Mol. Ecol , vol.10 , pp. 515-523
    • Tengs, T.1    Bowers, H.A.2    Ziman, A.P.3    Stoecker, D.K.4    Oldach, D.W.5
  • 31
    • 0002644592 scopus 로고
    • Preservation and storage
    • A. Sournia ed, UNESCO, Paris, France
    • Throndsen, J. 1978. Preservation and storage, p. 69-74. In A. Sournia (ed.), Phytoplankton manual. UNESCO, Paris, France.
    • (1978) Phytoplankton manual , pp. 69-74
    • Throndsen, J.1
  • 32
    • 0000954448 scopus 로고
    • Zur Vervollkommnung der quantitativen Phytoplankton-Methodik.
    • Utermöhl, H. 1958. Zur Vervollkommnung der quantitativen Phytoplankton-Methodik. Mitt. Int. Ver. Theor. Angew. Limnol. 9:1-38.
    • (1958) Mitt. Int. Ver. Theor. Angew. Limnol , vol.9 , pp. 1-38
    • Utermöhl, H.1
  • 33
    • 27844606990 scopus 로고    scopus 로고
    • Culturing and environmental DNA sequencing uncover hidden kinetoplastid biodiversity and a major marine clade within ancestrally freshwater Neobodo designis
    • von der Heyden, S., and T. Cavalier-Smith. 2005. Culturing and environmental DNA sequencing uncover hidden kinetoplastid biodiversity and a major marine clade within ancestrally freshwater Neobodo designis. Int. J. Syst. Evol. Microbiol. 55:2605-2621.
    • (2005) Int. J. Syst. Evol. Microbiol , vol.55 , pp. 2605-2621
    • von der Heyden, S.1    Cavalier-Smith, T.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.