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Volumn 42, Issue 2, 2008, Pages 16-20

Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior

Author keywords

[No Author keywords available]

Indexed keywords

AGROBACTERIUM; NICOTIANA EXCELSIOR; RHIZOBIUM;

EID: 42349099805     PISSN: 05643783     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (4)

References (20)
  • 1
    • 0030667922 scopus 로고    scopus 로고
    • Applications of green fluorescent protein in plants
    • P
    • Leffel S.M., Mabon S.A., Stewart C.N. Jr. Applications of green fluorescent protein in plants // Biotechniques. -1997.- 23. - P. 912-918.
    • (1997) Biotechniques , vol.23 , pp. 912-918
    • Leffel, S.M.1    Mabon, S.A.2    Stewart Jr., C.N.3
  • 2
    • 27944475132 scopus 로고    scopus 로고
    • Fluorescent proteins as a toolkit for in vivo imaging
    • P
    • Chudakov D.M., Lukyanov S., Lukyanov K.A. Fluorescent proteins as a toolkit for in vivo imaging // Trends Biotech. - 2005. - 23. - P. 605-613.
    • (2005) Trends Biotech , vol.23 , pp. 605-613
    • Chudakov, D.M.1    Lukyanov, S.2    Lukyanov, K.A.3
  • 4
    • 0030452512 scopus 로고    scopus 로고
    • Mutations that suppress the thermosensitivity of green fluorescent protein
    • P
    • Siemering K.R., Golbik R., Sever R., Haseloff J. Mutations that suppress the thermosensitivity of green fluorescent protein // Curr. Biol. - 1996. - 6. - P. 1653-1663.
    • (1996) Curr. Biol , vol.6 , pp. 1653-1663
    • Siemering, K.R.1    Golbik, R.2    Sever, R.3    Haseloff, J.4
  • 5
    • 0030989517 scopus 로고    scopus 로고
    • Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly
    • P
    • Haseloff J., Siemering K.R., Prasher D.C., Hodge S. Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly // Proc. Nat. Acad. Sci. USA. - 1997. - 94. - P. 2122-2127.
    • (1997) Proc. Nat. Acad. Sci. USA , vol.94 , pp. 2122-2127
    • Haseloff, J.1    Siemering, K.R.2    Prasher, D.C.3    Hodge, S.4
  • 6
    • 14844293838 scopus 로고    scopus 로고
    • Magnifection - a new platform for expressing recombinant vaccines in plants
    • P
    • Gleba Y., Klimyuk V., Marllonnet S. Magnifection - a new platform for expressing recombinant vaccines in plants // Vaccine. - 2005. - 23. - P. 2042-2048.
    • (2005) Vaccine , vol.23 , pp. 2042-2048
    • Gleba, Y.1    Klimyuk, V.2    Marllonnet, S.3
  • 7
    • 33846936026 scopus 로고    scopus 로고
    • Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression
    • P
    • Sheludko Y.V., Sindarovska Y.R., Gerasymenko I.M., Bannikova M.A., Kuchuk N. V. Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression // Biotechnol. Bioeng. -2007.- 96.- P. 608-614.
    • (2007) Biotechnol. Bioeng , vol.96 , pp. 608-614
    • Sheludko, Y.V.1    Sindarovska, Y.R.2    Gerasymenko, I.M.3    Bannikova, M.A.4    Kuchuk, N.V.5
  • 8
    • 2542448230 scopus 로고    scopus 로고
    • Engineering green fluorescent protein as a dual functional tag
    • P
    • Paramban R.I., Bugos R.C., Su W. W. Engineering green fluorescent protein as a dual functional tag // Biotechnol. Bioeng. - 2004. - 86. - P. 687-697.
    • (2004) Biotechnol. Bioeng , vol.86 , pp. 687-697
    • Paramban, R.I.1    Bugos, R.C.2    Su, W.W.3
  • 9
    • 0036752603 scopus 로고    scopus 로고
    • Protein purification via aqueous two-phase extraction (ATPE) and immobilized metal affinity chromatography. Effectiveness of salt addition to enhance selectivity and yield of GFPuv
    • P
    • Li Y. Beitle R.R. Protein purification via aqueous two-phase extraction (ATPE) and immobilized metal affinity chromatography. Effectiveness of salt addition to enhance selectivity and yield of GFPuv // Biotechnol. Prog. - 2002. - 18. - P. 1054-1059.
    • (2002) Biotechnol. Prog , vol.18 , pp. 1054-1059
    • Li, Y.1    Beitle, R.R.2
  • 10
    • 0032405581 scopus 로고    scopus 로고
    • Yakhnin A.V., Vinokurov L.M., Surin A.K., Alakhov Y.B. Green fluorescent protein purification by organic extraction// Protein Exp. Purif. - 1998. - 14. - P. 382-386.
    • Yakhnin A.V., Vinokurov L.M., Surin A.K., Alakhov Y.B. Green fluorescent protein purification by organic extraction// Protein Exp. Purif. - 1998. - 14. - P. 382-386.
  • 11
    • 0029347056 scopus 로고
    • Rapid purification of recombinant green fluorescent protein using the hydrophobic properties of an HPLC size-exclusion column
    • P
    • Deschamps J.R., Miller C.E., Ward K.B. Rapid purification of recombinant green fluorescent protein using the hydrophobic properties of an HPLC size-exclusion column // Protein Exp. Purif. - 1995. - 6. - P. 555-558.
    • (1995) Protein Exp. Purif , vol.6 , pp. 555-558
    • Deschamps, J.R.1    Miller, C.E.2    Ward, K.B.3
  • 12
    • 0035128545 scopus 로고    scopus 로고
    • Purification of recombinant green fluorescent protein using chro matofocusing with a pH gradient composed of multiple stepwise fronts
    • P
    • Narahari C.R., Randers-Eichhorn L., Strong J.C., Ramasubramanyan N., Rao G., Frey D.D. Purification of recombinant green fluorescent protein using chro matofocusing with a pH gradient composed of multiple stepwise fronts // Biotechnol. Prog. - 2001. - 17. -P. 150-160.
    • (2001) Biotechnol. Prog , vol.17 , pp. 150-160
    • Narahari, C.R.1    Randers-Eichhorn, L.2    Strong, J.C.3    Ramasubramanyan, N.4    Rao, G.5    Frey, D.D.6
  • 13
    • 2342525801 scopus 로고    scopus 로고
    • In planta engineering of viral RNA replicons: Efficient assembly by recombination of DNA modules delivered by Agrobacterium
    • P
    • Marillonnet S., Giritch A., Gils M., Kanzia R., Klimyuk V., Gleba Y. In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium // Proc. Nat. Acad. Sci. USA. - 2004. - 101. - P. 6852-6857
    • (2004) Proc. Nat. Acad. Sci. USA , vol.101 , pp. 6852-6857
    • Marillonnet, S.1    Giritch, A.2    Gils, M.3    Kanzia, R.4    Klimyuk, V.5    Gleba, Y.6
  • 14
    • 33744933668 scopus 로고    scopus 로고
    • Gils M., Kandzia R., Marillonnet S., Klimyuk V., Gleba Y. High-yield production of authentic human growth hormone using a plant virus-based expression system // Plant Biotechnol. J. - 2005. - 3. - P. 613-620.
    • Gils M., Kandzia R., Marillonnet S., Klimyuk V., Gleba Y. High-yield production of authentic human growth hormone using a plant virus-based expression system // Plant Biotechnol. J. - 2005. - 3. - P. 613-620.
  • 15
    • 24944498904 scopus 로고    scopus 로고
    • Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants
    • P
    • Marillonnet S., Thoeringer C., Kandzia R., Klimyuk V., Gleba Y. Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants // Nat. Biotechnol. - 2005. - 23. -P. 718-723.
    • (2005) Nat. Biotechnol , vol.23 , pp. 718-723
    • Marillonnet, S.1    Thoeringer, C.2    Kandzia, R.3    Klimyuk, V.4    Gleba, Y.5
  • 16
    • 0037342553 scopus 로고    scopus 로고
    • Voinnet O., Rivas S., Mestre P., Baulcombe D. An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus // Plant J. - 2003. - 33. -P. 949-956.
    • Voinnet O., Rivas S., Mestre P., Baulcombe D. An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus // Plant J. - 2003. - 33. -P. 949-956.
  • 17
    • 0030729369 scopus 로고    scopus 로고
    • Silencing of transgenes introduced into leaves by agroinfiltration: A simple, rapid method for investigating sequence requirements for gene silencing// Mol
    • P
    • Schob H., Kunz C., Meins F. Jr. Silencing of transgenes introduced into leaves by agroinfiltration: a simple, rapid method for investigating sequence requirements for gene silencing// Mol. Gen. Genet. - 1997. - 256. -P. 581-585.
    • (1997) Gen. Genet , vol.256 , pp. 581-585
    • Schob, H.1    Kunz, C.2    Meins Jr., F.3
  • 18
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein - dye binding
    • P
    • Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein - dye binding // Anal. Biochem. - 1976. - 72. - P. 248-254.
    • (1976) Anal. Biochem , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 19
    • 0017740440 scopus 로고
    • A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250
    • P
    • Blakesly R. W., Boezi J.A. A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250 // Anal. Biochem. - 1977. - 82. -P. 580-582.
    • (1977) Anal. Biochem , vol.82 , pp. 580-582
    • Blakesly, R.W.1    Boezi, J.A.2
  • 20
    • 15744368360 scopus 로고    scopus 로고
    • McRae S.R., Brown C.L., Bushell G.R. Rapid purification of EGFP, EYFP,and ECFP with high yield and purity// Protein Exp. Purif. - 2005. - 41. - P. 121-127.
    • McRae S.R., Brown C.L., Bushell G.R. Rapid purification of EGFP, EYFP,and ECFP with high yield and purity// Protein Exp. Purif. - 2005. - 41. - P. 121-127.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.