Correlative light/electron microscopy for the investigation of microbial mats from Black Sea Cold Seeps

Journal of Microbiological Methods

Volumn 73, Issue 2, 2008, Pages 85-91

  Wrede, Christoph ^a   Heller, Christina ^a   Reitner, Joachim ^a   Hoppert, Michael ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Biofilm; Electron microscopy; Fluorescence light microscopy; Lectin labeling

Indexed keywords

CARBONIC ACID; LECTIN; RESIN;

ARTICLE; BIOFILM; CONTROLLED STUDY; ELECTRON MICROSCOPY; IMMUNOLOCALIZATION; METHANOGENESIS; MICROBIAL MAT; MICROSCOPY; MUCILAGE; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

ARCHAEA; BACTERIA; CARBONATES; COLD; FLUORESCENT DYES; GEOLOGIC SEDIMENTS; GOLD COLLOID; LECTINS; METHANE; MICROSCOPY; MICROSCOPY, ELECTRON; MICROTOMY; OXIDATION-REDUCTION; PLASTIC EMBEDDING; POLYSACCHARIDES; POLYSACCHARIDES, BACTERIAL; SEAWATER; STAINING AND LABELING; SULFATES;

ARCHAEA; BACTERIA (MICROORGANISMS);

EID: 42249111033     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.02.020     Document Type: Article

References (21)

1. Armbruster B.L., Carlemalm E., Chiovetti R., Gravioti R.M., Hobot J.A., Kellenberger E., and Vilinger W. Specimen preparation for electron microscopy using low temperature embedding resin. J. Microsc. (Oxf.). 126 (1982) 77-92
2. Darcy K.J., Staras K., Collinson L.M., and Goda Y. An ultrastructural readout of fluorescence recovery after photobleaching using correlative light and electron microscopy. Nat. Protoc. 1 (2006) 988-994
3. Doddema H.J., and Vogels G.D. Improved identification of methanogenic bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 36 (1978) 752-754
4. Dupraz C., and Visscher P.T. Microbial lithification in marine stromatolites and hypersaline mats. Trends Microbiol. 13 (2005) 429-438
5. Hallam S.J., Putnam N., Preston C.M., Detter J.C., Rokhsar D., Richardson P.M., and DeLong E.F. Reverse methanogenesis: testing the hypothesis with environmental genomics. Science 305 (2004) 1457-1462
6. Hinrichs K.-U., and Boetius A. The anaerobic oxidation of methane: new insights in microbial ecology and biogeochemistry. In: Hebben D., Joergensen B.B., Schlüter M., and van Weering T. (Eds). Ocean Margin Systems (2002), Springer, Berlin 457-477
7. Hoppert M., and Holzenburg A. Electron Microscopy in Microbiology (Microscopy Handbooks) (1998), Bios Scientific Publishers (Bios-Springer), New York
8. Kämper M., Vetterkind S., Berker R., and Hoppert M. Methods for in-situ detection and characterization of extracellular polymers in biofilms by electron microscopy. J. Microbiol. Methods 57 (2004) 55-64
9. Knittel K., Lösekann T., Boetius A., Kort R., and Amann R. Diversity and distribution of methanotrophic archaea at cold seeps. Appl. Environ. Microbiol. 71 (2005) 467-479
10. Krüger M., Meyerdierks A., Glöckner F.O., Amann R., Widdel F., Kube M., Reinhardt R., Kahnt J., Böcher R., Thauer R.K., and Shima S. A conspicuous nickel protein in mircobial mats that oxidize methane anaerobically. Nature 426 (2003) 878-881
11. Lawrence J.R., Swerhone G.D., Kuhlicke U., and Neu T.R. In situ evidence for microdomains in the polymer matrix of bacterial microcolonies. Can. J. Microbiol. 53 (2007) 450-458
12. McRae R., Laj B., and Fahrni C.J. Correlative microXRF and optical immunofluorescence microscopy of adherent cells labelled with ultrasmall gold particles. J. Struct. Biol. 155 (2006) 22-29
13. Neu T.R., and Lawrence J.R. Lectin-binding analysis in biofilm systems. Methods Enzymol. 310 (1999) 145-152
14. Velasco A., and Hidalgo J. Light and electron microscopical localization of concanavalin A lectin binding sites in rat epiphyseal chondrocytes. Histochem. J. 19 (1987) 7-14
15. Peckmann J., Reimer A., Luth U., Luth C., Hansen B.T., Heinicke C., Hoefs J., and Reitner J. Methane-derived carbonates and authigenic pyrite from the northwestern Black Sea. Mar. Geol. 177 (2001) 129-150
16. Reitner J., Peckmann J., Blumenberg M., Michaelis W., Reimer A., and Thiel V. Concretionary methane-seep carbonates and associated microbial communities in Black Sea sediments. Palaeogeogr. Palaeoclimatol. Palaeoecol. 227 (2005) 18-30
17. Reitner J., Peckmann J., Reimer A., Schumann G., and Thiel V. Methane-derived carbonate build-ups and associated microbial communities at cold seeps on the lower Crimean shelf (Black Sea). Facies 51 (2005) 66-79
18. Robinson J.M., Takiyawa T., Pombo A., and Cook P.R. Correlative fluorescence and electron microscopy on ultrathin cryosections - bridging the resolution gap. J. Histochem. Cytochem. 49 (2001) 803-808
19. Robinson J.M., Takizawa T., and Vandre D.D. Applications of gold cluster compounds in immunocztochemistry and correlative microscopy - comparison with colloidal gold. J. Microsc. 199 (2000) 163-179
20. Roth J., Bendayan M., Carlemalm E., Villiger W., and Garavito M. Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J. Histochem. Cytochem. 29 (1981) 663-671
21. Takizawa T., and Robinson J.M. Ultrathin cryosections - an important tool for immunofluorescence and correlative microscopy. J. Histochem. Cytochem. 51 (2003) 707-714